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雞柏氏禽刺螨感染的初步研究

發(fā)布時間:2018-06-28 11:36

  本文選題:皮炎 + 柏氏禽刺螨; 參考:《中國獸醫(yī)科學(xué)》2017年03期


【摘要】:為探索四川某雞場近年來肉雞每年夏季均發(fā)生以腹部、腿部皮膚出現(xiàn)丘疹與痂皮為特征性病變的疫病病因,根據(jù)送檢病雞的臨床癥狀、剖檢病變和疾病流行特點進(jìn)行初步診斷,并采用常規(guī)實驗室診斷方法和PCR技術(shù)進(jìn)行寄生蟲檢查、細(xì)菌分離鑒定、鑒別診斷和治療的初步研究。結(jié)果顯示,從病雞體表檢查到的禽刺螨為柏氏禽刺螨,PCR擴(kuò)增其線粒體細(xì)胞色素氧化酶亞單位1(CO 1)基因的576 bp大小靶片段與柏氏禽刺螨OB株核苷酸序列的同源性為99%;同時從丘疹病變組織中分離到1株阿尼蒂斯葡萄球菌,該葡萄球菌分離株不致死昆明小鼠,耐受青霉素、阿莫西林、環(huán)丙沙星、頭孢他啶、復(fù)方新諾明、卡那霉素和強(qiáng)力霉素等13種受試藥物;確定此疫病由雞柏氏禽刺螨感染引起,滅鼠、殺螨和敏感藥物抗菌措施具有良好防治效果。這是國內(nèi)雞發(fā)生柏氏禽刺螨感染的首次報道,為雞禽刺螨感染的防控及相關(guān)研究提供了科學(xué)參考。
[Abstract]:In order to explore the pathogeny of the disease of the chicken in a chicken farm in Sichuan in recent years, the disease of the abdomen, the skin of the leg and the skin of the scab, the disease and the epidemic characteristics of the disease were diagnosed according to the clinical symptoms of the infected chickens, and the common laboratory diagnosis method and the PCR technique were used to examine the parasites. A preliminary study of isolation, identification, differential diagnosis and treatment. The results showed that the avian acarinus from the body of the sick chicken was PAT'S acarinus, and the PCR amplification of the 576 BP target fragment of the mitochondrial cytochrome oxidase subunit 1 (CO 1) gene was 99% of the nucleotide sequence of the OB strain of the acarinoid mites of the avian acarinus mites, and was isolated from the papular lesion tissue. To 1 strains of aniti Staphylococcus, the staphylococcal isolate did not kill Kunming mice, tolerated penicillin, amoxicillin, ciprofloxacin, ceftazidime, compound novomine, kanamycin and doxycycline, and other 13 tested drugs; it was determined that the epidemic is good for the infection of chicken PAT'S acarinosus, deratization, mite killing and sensitive drugs. This is the first report on the occurrence of PAT'S acari infection in domestic chicken, which provides a scientific reference for the prevention and control of acari infection and related research.
【作者單位】: 四川農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院;成都市動物疫病預(yù)防控制中心;四川農(nóng)業(yè)大學(xué)動物科技學(xué)院;
【基金】:四川省科技計劃項目(2016NZYZF0059) “十二五”國家科技支撐計劃項目(2013BAD12B04)
【分類號】:S858.31
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本文編號:2077865

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