纖維素降解菌的篩選及在狼尾草青貯中使用效果評(píng)價(jià)
發(fā)布時(shí)間:2018-06-25 08:41
本文選題:纖維素降解菌 + 酶活力; 參考:《草業(yè)學(xué)報(bào)》2016年04期
【摘要】:纖維素降解菌對(duì)改善牧草品質(zhì)和提高動(dòng)物對(duì)飼草的利用率具有重要作用。引進(jìn)多種纖維素降解菌并從中篩選出酶活力較高的11,21和36號(hào)菌株。利用篩選出的3株菌株的發(fā)酵液,開(kāi)展在青貯雜交狼尾草中的使用效果評(píng)價(jià)[設(shè)4個(gè)處理,處理1為未添加菌液的對(duì)照組(CK),處理2,3,4分別添加11,21,36號(hào)菌株菌液]。結(jié)果表明,添加纖維素降解菌能提高青貯牧草乳酸含量,處理2,3,4的乳酸含量比CK提高了19.42%(P0.05),38.35%(P0.05)和4.85%(P0.05);添加纖維素菌液進(jìn)行青貯的3個(gè)處理的中性洗滌纖維(NDF)、酸性洗滌纖維(ADF)和半纖維素(HC)的含量均低于CK和青貯前,其中處理3的NDF、ADF和HC含量最低,表明篩選出的3種纖維素降解菌菌株均能改善青貯品質(zhì),不同程度地降解了牧草纖維素的含量,其中處理3(21號(hào)菌株)降解纖維素的效果最為明顯,青貯料營(yíng)養(yǎng)品質(zhì)最好。
[Abstract]:Cellulose degrading bacteria play an important role in improving forage quality and animal utilization rate of forage. A variety of cellulose-degrading bacteria were introduced and screened out from them. The fermentation broth of 3 strains was used to evaluate the effect of the fermentation in silage hybrids. [four treatments were set up, one was the control group (CK) without adding the bacterial liquid, and the other was treated with the strain 1121,36 respectively]. The results showed that the addition of cellulose degrading bacteria could increase the content of lactic acid in silage. Compared with CK, the content of lactic acid increased by 19.42% (P0.05) and 4.85% (P0.05), and the contents of NDF, ADF and HC were lower than those of CK and pre-silage. The content of ADF and HC in treatment 3 was the lowest, which indicated that the three strains of cellulose-degrading bacteria could improve the silage quality and degrade the cellulose content of forage to varying degrees. Among them, treatment 3 (strain 21) had the most obvious effect on the degradation of cellulose, and the silage had the best nutritional quality.
【作者單位】: 福建省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;福建省丘陵地區(qū)循環(huán)農(nóng)業(yè)工程技術(shù)研究中心;福建省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)生態(tài)研究所;
【基金】:“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2014BAD15B01,2012BAD14B15-03,2011BAD17B02-06) 農(nóng)業(yè)部公益性行業(yè)科研專(zhuān)項(xiàng)(201303094-04) 福建省自然基金(2013J01105)資助
【分類(lèi)號(hào)】:S816.53
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