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纖維素降解菌的篩選及在狼尾草青貯中使用效果評價

發(fā)布時間:2018-06-25 08:41

  本文選題:纖維素降解菌 + 酶活力 ; 參考:《草業(yè)學報》2016年04期


【摘要】:纖維素降解菌對改善牧草品質和提高動物對飼草的利用率具有重要作用。引進多種纖維素降解菌并從中篩選出酶活力較高的11,21和36號菌株。利用篩選出的3株菌株的發(fā)酵液,開展在青貯雜交狼尾草中的使用效果評價[設4個處理,處理1為未添加菌液的對照組(CK),處理2,3,4分別添加11,21,36號菌株菌液]。結果表明,添加纖維素降解菌能提高青貯牧草乳酸含量,處理2,3,4的乳酸含量比CK提高了19.42%(P0.05),38.35%(P0.05)和4.85%(P0.05);添加纖維素菌液進行青貯的3個處理的中性洗滌纖維(NDF)、酸性洗滌纖維(ADF)和半纖維素(HC)的含量均低于CK和青貯前,其中處理3的NDF、ADF和HC含量最低,表明篩選出的3種纖維素降解菌菌株均能改善青貯品質,不同程度地降解了牧草纖維素的含量,其中處理3(21號菌株)降解纖維素的效果最為明顯,青貯料營養(yǎng)品質最好。
[Abstract]:Cellulose degrading bacteria play an important role in improving forage quality and animal utilization rate of forage. A variety of cellulose-degrading bacteria were introduced and screened out from them. The fermentation broth of 3 strains was used to evaluate the effect of the fermentation in silage hybrids. [four treatments were set up, one was the control group (CK) without adding the bacterial liquid, and the other was treated with the strain 1121,36 respectively]. The results showed that the addition of cellulose degrading bacteria could increase the content of lactic acid in silage. Compared with CK, the content of lactic acid increased by 19.42% (P0.05) and 4.85% (P0.05), and the contents of NDF, ADF and HC were lower than those of CK and pre-silage. The content of ADF and HC in treatment 3 was the lowest, which indicated that the three strains of cellulose-degrading bacteria could improve the silage quality and degrade the cellulose content of forage to varying degrees. Among them, treatment 3 (strain 21) had the most obvious effect on the degradation of cellulose, and the silage had the best nutritional quality.
【作者單位】: 福建省農(nóng)業(yè)科學院畜牧獸醫(yī)研究所;福建省丘陵地區(qū)循環(huán)農(nóng)業(yè)工程技術研究中心;福建省農(nóng)業(yè)科學院農(nóng)業(yè)生態(tài)研究所;
【基金】:“十二五”國家科技支撐計劃項目(2014BAD15B01,2012BAD14B15-03,2011BAD17B02-06) 農(nóng)業(yè)部公益性行業(yè)科研專項(201303094-04) 福建省自然基金(2013J01105)資助
【分類號】:S816.53

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1 史國翠;尹文新;李明軒;張敏;;纖維素降解菌的篩選與鑒定[J];黑龍江畜牧獸醫(yī);2010年19期

2 王慧;趙世華;李云章;程超;巴雅爾其木格;;駱駝瘤胃內優(yōu)勢纖維素降解菌酶活種類及活力測定方法比較研究[J];畜牧與飼料科學;2014年01期

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