本文選題：犬 + MG�。� 參考：《中國獸醫(yī)科學》2017年01期

【摘要】：為進一步研究犬MG53在機體內的分布情況及其生物活性,本研究設計特異性引物進行犬MG53目的基因的克隆,并將其連接至p MAL-C5X表達載體中,利用大腸桿菌表達系統(tǒng)進行表達。結果顯示,克隆得到由1 435個核苷酸構成的犬MG53基因;獲得MG53蛋白與麥芽糖結合蛋白標簽(MBP)融合表達的可溶性重組蛋白,大小約為95 ku。通過結構及生物學功能預測發(fā)現,該蛋白易降解,具有RING、B-Box、Coiledcoil及SPRY-PRY四個功能結構域,并分布有半胱胺(Cysteamine)、2-氨基乙硫醇(2-Amino-1-Ethanethiol)、硫代乙醇胺(Thioethanolamine)、2,3-Deshydrolanthionine、Zn2+及Mg2+等分子結合位點,具有蛋白結合能力。上述結果表明,犬MG53與人、家兔、小鼠、大鼠MG53的結構相似,并可能具有參與泛素化、骨骼肌細胞修復等重要的生物學功能。
[Abstract]:In order to further study the distribution and biological activity of dog MG53, a specific primer was designed to clone the target gene of dog MG53, which was ligated into pMAL-C5X expression vector and expressed by E. coli expression system. The results showed that the canine MG53 gene composed of 1,435 nucleotides was cloned and the soluble recombinant protein expressed by fusion of MG53 protein and maltose binding protein label (MBP) was obtained, the size of which was about 95 ku. It was found that the protein was easy to degrade and had four functional domains RINGB-Boxen Coiledcoil and SPRY-PRY. The protein was characterized by cysteamine 2-aminoethiol thioethanolamine 2 3-Deshydrolanthionine Zn2 and Mg2. These results suggest that the structure of dog MG53 is similar to that of human, rabbit, mouse and rat MG53, and may have important biological functions such as ubiquitization, skeletal muscle cell repair and so on.
【作者單位】： 華南農業(yè)大學獸醫(yī)學院廣東省獸醫(yī)臨床重大疾病綜合防控重點實驗室廣東省寵物工程技術研究工程中心;
【基金】：廣東省高等學校優(yōu)秀青年教師培養(yǎng)計劃資助項目(YQ2015030) 廣東省獸醫(yī)臨床重大疾病綜合防控重點實驗室資助項目(2013A061401013)
【分類號】：S829.2

【相似文獻】

相關期刊論文 前2條

1 郭紅燕;王沖;郭愛珍;顏邦芬;陳煥春;;貓血管緊張素轉換酶2的三維結構模擬與真核表達[J];華中農業(yè)大學學報;2007年01期

2 ;[J];;年期

，

本文編號：2065173