本文選題：鉻(Ⅵ) + 硒　； 參考：《山東農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：近年來,對(duì)機(jī)體微量元素的研究已經(jīng)成為熱點(diǎn)。本實(shí)驗(yàn)的目的是探究不同濃度的硒對(duì)六價(jià)鉻造成的肝損傷的促進(jìn)或者抑制作用。首先進(jìn)行急性動(dòng)物實(shí)驗(yàn),測得K_2Cr_2O_7得LD50為369mg/kg,選擇6%LD50作為本次試驗(yàn)的攻毒劑量,即為22.14mg/kg。慢性動(dòng)物實(shí)驗(yàn),選用105只7日齡海蘭褐雞,隨機(jī)分為七組,通過飲水供毒,第Ⅰ組為空白組,飲用去離子水,第Ⅱ組飲水中添加22.14mg/kg的K_2Cr_2O_7,第Ⅲ組添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(0.31 mg/kg),第Ⅳ組添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(0.63 mg/kg),第Ⅴ組添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(1.25 mg/kg),第Ⅵ組添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(2.50 mg/kg),第Ⅶ組添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(5.00mg/kg),各組試劑連續(xù)添加42d。在驗(yàn)的14d,28d,42d采集肝臟樣品,檢測肝體比、丙二醛(MDA)含量、谷胱甘肽(GSH)含量、總超氧化物歧化酶(T-SOD)活性、Ca~(2+)-ATP酶活性和線粒體膜電位(MMP)變化,觀察肝組織病理切片。結(jié)果表明,飲水中添加六價(jià)鉻會(huì)導(dǎo)致雞肝臟生長發(fā)育受阻,肝組織切片能觀察到病理損傷。六價(jià)鉻引起MDA含量增加,GSH含量減少,T-SOD活性和Ca~(2+)-ATP活性降低,MMP下降,并且各組數(shù)據(jù)與對(duì)照組差異極顯著(P0.01)。同時(shí),飲水中添加六價(jià)鉻的同時(shí)分別補(bǔ)充0.31mg/kg、0.62mg/kg和1.25mg/kg硒時(shí),能夠抑制六價(jià)鉻所引起的肝臟損傷,降低MDA含量,增加GSH含量,升高T-SOD和Ca~(2+)-ATP酶活性,使MMP上升,其中以添加0.63 mg/kg的硒效果最佳。飲水中添加六價(jià)鉻的同時(shí)補(bǔ)充2.50 mg/kg和5.00mg/kg的硒時(shí),能加重六價(jià)鉻所誘導(dǎo)的肝臟損傷,肝臟組織中MDA含量進(jìn)一步增多,GSH含量降低,T-SOD和Ca~(2+)-ATP酶活性降低,MMP下降。實(shí)驗(yàn)結(jié)果表明,一定劑量的K_2Cr_2O_7能導(dǎo)致肝損傷,而且飲水中添加適量的硒對(duì)Cr(Ⅵ)造成的肝損傷具有保護(hù)作用,過量的硒則加重Cr(Ⅵ)造成的肝損傷。
[Abstract]:In recent years, the research on trace elements has become a hot spot. The aim of this study was to investigate the effects of selenium at different concentrations on liver injury induced by hexavalent chromium. First, the acute animal experiment was carried out, and the LD50 was determined to be 369 mg / kg, and 6LD50 was selected as the dose of 22.14 mg / kg. In the chronic animal experiment, 105 7-day-old Hailan brown chickens were randomly divided into seven groups, which were fed by drinking water. The first group was a blank group, drinking deionized water. 絎叀緇勯ギ姘翠腑娣誨姞22.14mg/kg鐨凨_2Cr_2O_7,絎參緇勬坊鍔燢_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(0.31 mg/kg),絎叄緇勬坊鍔燢_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(0.63 mg/kg),絎叅緇勬坊鍔燢_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(1.25 mg/kg),絎叆緇勬坊鍔燢_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(2.50 mg/kg),絎叇緇勬坊鍔燢_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(5.00mg/kg),鍚勭粍璇曞墏榪炵畫娣誨姞42d. Liver samples were collected on the 14th day after 28 days. The liver body ratio, malondialdehyde (MDA) content, glutathione (GSH) content, total superoxide dismutase (T-SOD) activity and mitochondrial membrane potential (MMP) were observed. The results showed that adding hexavalent chromium to drinking water could lead to the inhibition of liver growth and development, and pathological damage could be observed in liver tissue sections. The content of MDA and GSH were increased by hexavalent chromium. The activity of T-SOD and Ca ~ (2) -ATP decreased, and the data of each group were significantly different from the control group (P0.01). At the same time, adding hexavalent chromium and adding 0.31 mg / kg 0.62 mg / kg and 1.25mg/kg selenium in drinking water could inhibit the liver injury induced by hexavalent chromium, decrease the content of MDA, increase the content of GSH, increase the activities of T-SOD and Ca ~ (2) -ATPase, and increase the activity of MMP. Among them, the effect of adding 0.63 mg/kg selenium was the best. Addition of chromium hexavalent to drinking water supplemented with selenium of 2.50 mg/kg and 5.00mg/kg increased the liver damage induced by Cr, and further increased the content of GSH in the liver and decreased the activities of T-SOD and Ca ~ (2) -ATPase. The experimental results showed that a certain dose of K _ S _ 2Cr _ 2O _ 7 could lead to liver injury, and the addition of proper amount of selenium to drinking water could protect the liver from Cr (鈪，

本文編號(hào)：2063118