本文選題：鴨 + 產(chǎn)蛋性狀��； 參考：《華中農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本研究以卵抑制劑(OIH)基因和促卵泡激素(FSHβ)基因?yàn)轼啴a(chǎn)蛋性狀相關(guān)候選基因,先克隆獲得了兩基因部分序列,并采用DNA池技術(shù)檢測(cè)了兩基因的DNA多態(tài)性,兩個(gè)基因各選擇兩個(gè)SNP位點(diǎn)做PCR-RFLP,以連城白鴨與白改鴨雜交第3代群體為材料,檢測(cè)各個(gè)體的基因型并研究性狀的關(guān)聯(lián),主要研究結(jié)果如下:1.克隆獲得了鴨卵抑制劑(OIH)基因Exon10-Exon12和Intorn10-Intorn12的部分序列,采用DNA池加測(cè)序的方法對(duì)鴨卵抑制劑(OIH)基因編碼區(qū)及其側(cè)翼序列進(jìn)行SNP掃描,共檢測(cè)到4個(gè)新的SNP位點(diǎn),其中位點(diǎn)c.316AG和c.1287 AC分別導(dǎo)致了限制性內(nèi)切酶Acc I和Alu I酶切位點(diǎn)多態(tài)性的改變。在連城白鴨與白改鴨雜交第3世代群體中,c.316AG位點(diǎn)等位基因G和A頻率分別為0.33和0.67,c.1287 AC位點(diǎn)等位基因C和A頻率分別為0.91和0.09;關(guān)聯(lián)分析結(jié)果顯示,位點(diǎn)c.316AG與群體的420天產(chǎn)蛋量(P=0.05),蛋殼強(qiáng)度(P0.05)存在顯著相關(guān),與390天產(chǎn)蛋量(P=0.08)相關(guān)程度接近顯著水平;位點(diǎn)c.1287 AC與連城白鴨與白改鴨雜交群體的270天產(chǎn)蛋量(P0.05),300天產(chǎn)蛋量(P0.05),330天產(chǎn)蛋量(P0.01),360天產(chǎn)蛋量(P0.01),390天產(chǎn)蛋量(P0.01),420天產(chǎn)蛋量(P0.01)存在顯著相關(guān)或極顯著相關(guān)。2.擴(kuò)增獲得了鴨促卵泡激素(FSHβ)基因第三外顯子部分編碼區(qū)序列;在鴨FSHβ基因序列中共檢測(cè)到7個(gè)新的SNP位點(diǎn),其中位點(diǎn)c.82CG和c.308TG分別導(dǎo)致Alu I和Ban II酶切位點(diǎn)的改變;在連城白鴨與白改鴨雜交第3世代群體中,c.82CG位點(diǎn)等位基因C和G頻率分別為0.24和0.76,c.308TG位點(diǎn)等位基因T和G分別為0.64和0.36;性狀關(guān)聯(lián)分析結(jié)果表明,位點(diǎn)c.82CG與連城白鴨與白改鴨雜交群體的開產(chǎn)蛋重(P0.01)、蛋白高度(P0.05)存在顯著或極顯著相關(guān);位點(diǎn)c.308TG與連城白鴨與白改鴨雜交群體的120天產(chǎn)蛋量(P0.01)存在極顯著相關(guān),與開產(chǎn)日齡(P=0.06)相關(guān)性接近顯著水平。3.利用PHASE軟件對(duì)以上兩基因突變位點(diǎn)進(jìn)行了單倍型重構(gòu),在連城白鴨與白改鴨雜交第3世代群體中,性狀關(guān)聯(lián)分析結(jié)果表明,OIH基因重構(gòu)二倍體型與連城白鴨與白改鴨雜交群體的后期產(chǎn)蛋量(EP330d,EP360d,EP390d,EP420d)存在顯著相關(guān)(P0.05),FSHβ基因重構(gòu)二倍體型與連城白鴨與白改鴨雜交群體的早期產(chǎn)蛋量(EP120d,EP180d,EP210d,EP240d)存在顯著(P0.05)或者極顯著(P0.01)相關(guān)。
[Abstract]:In this study, OIH gene and follicle stimulating hormone (FSH 尾) gene were used as candidate genes for duck egg-laying traits. The partial sequences of the two genes were cloned, and the DNA polymorphisms of the two genes were detected by DNA pool technique. Two SNP loci of each gene were selected as PCR-RFLP.The third generation population of Liancheng White Duck and White modified Duck were used as materials to detect the genotypes of each individual and study the correlation of traits. The main results were as follows: 1. The partial sequences of duck egg inhibitor (OIH) gene Exon10-Exon12 and Intorn10-Intorn12 were cloned. The coding region and flanking sequence of duck egg inhibitor (OIH) gene were scanned by DNA pool plus sequencing, and four new SNP sites were detected. Among them, locus c.316AG and c.1287AC resulted in the polymorphism of restriction endonuclease Acc I and Alu I, respectively. In the third generation population of Liancheng White Duck and White modified Duck, the alleles G and A at allele C and A were 0.33 and 0.67 c. 1287, respectively, and the correlation analysis showed that the allele C and A were 0.91 and 0.09, respectively. Locus c.316AG was significantly correlated with egg production (P0. 05) and eggshell strength (P0.05), and was close to significant level (P0. 08). There was significant correlation or extremely significant correlation between the number of eggs laid in 270 days (P0.05) and the number of eggs laid in 330 days (P0.01), 360 days (P0.01), 390 days (P0.01) and 420 days (P0.01) of Liancheng White Duck (P0.01) and Liancheng White Duck (P0.01). The partial coding region of the third exon of the duck follicle stimulating hormone (FSH 尾) gene was obtained, and seven new SNPs were detected in the FSH 尾 gene sequence, in which c. 82CG and c. 308TG led to the change of Alu I and Ban II endonuclease sites, respectively. In the third generation population of Liancheng White Duck and White modified Duck, the alleles C and G at allele C and G were 0.24 and 0.76 and 0.64 and 0.36, respectively, and the results of trait correlation analysis showed that the allele C and G were 0.64 and 0.36, respectively. Locus c.82CG was significantly correlated with egg weight (P0.01) and protein height (P0.05) of Liancheng white duck crossing population, and locus c.308TG was significantly correlated with 120-day egg production (P0.01) of Liancheng white duck cross population. The correlation with the age of birth (P0. 06) was close to the significant level of 3. 3. Haplotype reconstruction of the mutation sites of the two genes was carried out by phase software in the third generation population of Liancheng White Duck and White modified Duck. The results of trait association analysis showed that there was a significant correlation between the late egg production (EP330d / EP360d / EP390d/ EP420d) between Liancheng White Duck and Liancheng White Duck (P0.05) and Liancheng White Duck (Licheng White Duck) population by FSH 尾 gene reconstruction diploid type and Liancheng white duck cross population (EP330d / EP360d / EP390d / EP420d). Egg production (EP120 d, EP180 d, EP210 d, EP240 d) was significantly (P0.05) or extremely significant (P0.01).
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S834

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