本文選題：脂滴 + 小鼠��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：子宮頸是雌性動物維持妊娠和進(jìn)行分娩的重要器官,子宮頸的成熟對引產(chǎn)成功與否、分娩是否順利以及產(chǎn)程的長短來說都至關(guān)重要。子宮頸成熟是受到許多細(xì)胞因子和激素的調(diào)節(jié),是一個生化過程與炎癥反應(yīng)過程。子宮體也是雌性動物重要的生殖器官,它是孕育胎兒的場所,在精子遷移、受精卵著床以及胚胎、胎兒的生長發(fā)育及分娩過程中具有不可替代的作用。子宮頸中含有成纖維細(xì)胞、上皮細(xì)胞、內(nèi)皮細(xì)胞以及前脂肪細(xì)胞和脂肪細(xì)胞。成纖維細(xì)胞來源于胚胎時期的中胚層,是結(jié)締組織內(nèi)的主要細(xì)胞之一。具有一定的分化能力,并參與創(chuàng)傷修復(fù)、誘導(dǎo)成骨和成脂肪過程,還在一定條件下與纖維細(xì)胞和間充質(zhì)干細(xì)胞進(jìn)行相互轉(zhuǎn)化。脂肪組織還可作為內(nèi)分泌器官參與集體的生理和病理過程。前脂肪細(xì)胞或脂肪細(xì)胞就能夠分泌其中的細(xì)胞因子和激素,而本課題組就在分娩山羊的子宮頸中發(fā)現(xiàn)了含有大脂滴的脂肪細(xì)胞。在小鼠的子宮頸中也發(fā)現(xiàn)了含大脂滴的脂肪細(xì)胞的存在,并對小鼠子宮頸中脂滴的數(shù)量、分布以及變化等做了相應(yīng)的研究,取得了一定的成果。脂滴最初被認(rèn)為僅是用來儲存能量的一種顆粒,這種顆粒類似于糖原,是一種“惰性”的細(xì)胞內(nèi)物質(zhì),但隨著脂滴蛋白質(zhì)組學(xué)研究的不斷深入,研究人員發(fā)現(xiàn),脂滴還是一個復(fù)雜、動態(tài)變化、活動旺盛的多功能細(xì)胞器。它能沿著細(xì)胞的骨架運動,并能夠與其它細(xì)胞器相互作用,可能在信號傳導(dǎo)、膜轉(zhuǎn)運、脂類存儲與代謝等過程中扮演著非常重要的角色。脂滴在脂肪細(xì)胞、肝細(xì)胞、肌肉細(xì)胞以及腎上腺皮質(zhì)細(xì)胞等多種組織細(xì)胞中廣泛存在。本實驗以昆明小白鼠為研究對象,對小鼠子宮頸組織中脂滴是否受激素的影響進(jìn)行研究。采集成年小鼠子宮頸和經(jīng)摘除卵巢注射激素(雌二醇、孕酮、地塞米松和米非司酮)處理的小鼠子宮頸,將子宮頸做冰凍切片并油紅O染色;在潔凈工作臺內(nèi)采集小鼠子宮頸,在含有胎牛血清的DMEM培養(yǎng)基中培養(yǎng),并在培養(yǎng)基中加入一定濃度的激素,分別在無血清無激素、有血清無激素和有血清激含素濃度分別是10 nmol/L、20 nmol/L、40 nmol/L、80 nmol/L的四種激素的培養(yǎng)基中培養(yǎng),油紅O染色觀察。結(jié)果顯示:注射孕酮和地塞米松的小鼠的子宮頸中陽性率較高,注射米非司酮的陽性率較低;未摘卵巢未注射激素的小鼠的子宮體中陽性率最高,其他各組與子宮頸的結(jié)果基本一致。細(xì)胞培養(yǎng)時加孕酮的培養(yǎng)基培養(yǎng)的細(xì)胞中脂滴較加米非司酮的細(xì)胞中的脂滴含量高,經(jīng)含地塞米松的培養(yǎng)基培養(yǎng)細(xì)胞中的脂滴的含量也高于在含米非司酮的培養(yǎng)基中培養(yǎng)的細(xì)胞脂滴含量。以上結(jié)果表明,10、20、40 nmo/L孕酮和地塞米松能夠促進(jìn)脂滴的形成,米非司酮抑制脂滴的形成,20、40、80 nmol/L雌二醇對脂滴的形成有促進(jìn)作用,10 nmol/L雌二醇沒有明顯的差異性。
[Abstract]:Cervix is an important organ for female animals to maintain pregnancy and labor. The maturity of cervix is very important to the success of induced labor, the smooth delivery and the length of labor. Cervix maturation is regulated by many cytokines and hormones. It is a biochemical and inflammatory process. The uterine body is also an important reproductive organ of female animals. It is the place where the fetus is conceived. It plays an irreplaceable role in sperm migration, fertilized egg implantation and embryo, the growth, development and delivery of the fetus. The cervix contains fibroblasts, epithelial cells, endothelial cells and preadipocytes and adipocytes. Fibroblasts, derived from the mesoderm of embryonic stage, are one of the main cells in connective tissue. It has the ability of differentiation, participates in wound repair, induces osteogenesis and adipogenesis, and intertransforms with fibroblasts and mesenchymal stem cells under certain conditions. Adipose tissue can also be used as an endocrine organ to participate in collective physiological and pathological processes. Preadipocytes or adipocytes can secrete cytokines and hormones, and fat cells with large fat droplets have been found in the cervix of parturient goats. The presence of fat cells containing large lipid droplets was also found in the cervix of mice, and the number, distribution and changes of lipid droplets in the cervix of mice were studied accordingly, and some achievements were obtained. Lipid droplets were originally thought to be just particles for storing energy, similar to glycogen, an "inert" intracellular substance, but as the proteomics of lipid droplets progressed, researchers found, Lipid droplets are also a complex, dynamic, and active multifunctional organelle. It can move along the cytoskeleton and interact with other organelles, which may play a very important role in signal transduction, membrane transport, lipid storage and metabolism. Lipid droplets are widely found in adipocytes, hepatocytes, muscle cells and adrenal cortical cells. In this study, Kunming mice were used to study whether lipid droplets were affected by hormones in mouse cervix. The cervix of adult mice and mice treated with ovariectomized injection hormones (estradiol, progesterone, dexamethasone and mifepristone) were collected. Cultured in DMEM medium containing fetal bovine serum, and adding certain concentration of hormone to the medium, respectively, no hormone was found in serum. The concentrations of serum hormone free and serum excitins were 10 nmol / L ~ (20) nmol / L ~ (40) nmol / L ~ (80) nmol / L respectively, and oil red O staining was observed. The results showed that the positive rate in cervix of mice injected with progesterone and dexamethasone was higher than that of mice injected with mifepristone. The results of the other groups were consistent with those of the cervix. The lipid droplets in the cells cultured in the medium supplemented with progesterone were higher than those in the cells treated with mifepristone. The content of lipid droplets in the culture medium containing dexamethasone was also higher than that in the medium containing mifepristone. The results showed that progesterone and dexamethasone could promote the formation of lipid droplets. Mifepristone inhibited lipid droplet formation. Estradiol (estradiol) could promote the formation of lipid droplets. There was no significant difference between estradiol (estradiol) and estradiol (10nmol / L).
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S857.2

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本文編號：2038294