本文選題：納米蜂膠黃酮 + 豬細小病毒　； 參考：《中國獸醫(yī)學報》2017年01期

【摘要】：采用體內(nèi)外試驗研究納米蜂膠黃酮抗豬細小病毒(PPV)作用。體外試驗是以普通蜂膠黃酮為對照,分3種加藥方式(先加蜂膠、后加蜂膠、蜂膠和病毒同時加入)加至PK-15細胞中,用MTT法檢測細胞活性,實時熒光PCR檢測PK-15中PPV含量,觀察蜂膠黃酮對PK-15抗PPV及清除PPV能力的影響。體內(nèi)試驗是給豚鼠注射PPV后灌胃納米蜂膠黃酮和普通蜂膠黃酮,檢測肺臟、腎臟、肝臟、性腺及血液中PPV含量、血清中HI含量。結果顯示:(1)與普通蜂膠黃酮相比,納米蜂膠黃酮可顯著抑制PPV在PK-15上生長,高濃度效果優(yōu)于低濃度,3種加藥方式中先加藥物的效果最好;(2)高中劑量的納米蜂膠黃酮可以顯著抑制PPV在血液中復制、減輕PPV對豚鼠體重的影響、提高血清HI效價。結果表明:蜂膠黃酮經(jīng)納米化處理后,可以顯著提高其抗PPV活性。
[Abstract]:The effect of nanometer propolis flavonoids on porcine parvovirus PPVs was studied in vitro and in vivo. In vitro experiments were carried out using common propolis flavone as control. The cells were added to PK-15 cells by adding propolis first, then propolis, propolis and virus. The activity of PK-15 cells was detected by MTT assay, and the PPV content in PK-15 cells was detected by real-time fluorescence PCR. To observe the effect of propolis flavonoids on PK-15 anti-PPV and PPV scavenging ability. After PPV was injected into guinea pigs, the contents of PPV in lung, kidney, liver, gonad and blood, and HI in serum were measured by intragastric administration of nano-propolis flavonoids and ordinary propolis flavonoids. The results showed that compared with ordinary propolis flavonoids, nano-propolis flavonoids could significantly inhibit the growth of PPV on PK-15. The effect of high concentration was better than that of low concentration. (2) Nano-propolis flavonoids could significantly inhibit PPV replication in blood, reduce the effect of PPV on guinea pig body weight, and improve serum HI titer. The results showed that the anti-PPV activity of propolis flavonoids was significantly increased after nanocrystalline treatment.
【作者單位】： 山東省濱州畜牧獸醫(yī)研究院博士后科研工作站;吉林大學博士后科研流動站;河南牧業(yè)經(jīng)濟學院;山東省濱州畜牧獸醫(yī)研究院;
【基金】：山東省自然科學基金資助項目(2011ZRA16002) 山東省濱州畜牧獸醫(yī)研究院博士后科研啟動基金資助項目(BZXMBSH2014002)
【分類號】：S852.651

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