發(fā)布時間：2018-06-16 18:44

  本文選題：犬星狀病毒 + 分子流行病學(xué)��； 參考：《廣西大學(xué)》2017年碩士論文

【摘要】：犬星狀病毒(CanineAstrovirus,CAstV)是引發(fā)犬胃腸炎疾病的病原之一。近年來,該病毒在患有腸道腹瀉犬中被確定,序列分析發(fā)現(xiàn)其屬于獨有的星狀病毒物種,但是該病原在寵物犬中的臨床表現(xiàn)形式及其流行規(guī)律,目前尚未清楚。因此,本研究旨在了解CAstV在寵物犬群中的臨床流行特征和基因型分布特點,為進一步治療該疾病和了解CAstV流行規(guī)律提供科學(xué)依據(jù)。本研究主要收集2015年11月-2016年08月不同臨床特征、不同性別、不同年齡寵物犬群的253份糞便樣品。利用RT-巢式PCR(RT-nPCR)方法對CAstV進行臨床流行病學(xué)調(diào)查。研究發(fā)現(xiàn),患有腹瀉的犬只CAstV檢測陽性率高達25.3%,進一步證實腹瀉癥狀與CAstV感染的相關(guān)性。此外,對不同品種、性別和生長階段的寵物犬感染CAstVs進行分析調(diào)查,結(jié)果發(fā)現(xiàn),不同品種和性別的寵物犬都能可以感染該病毒,其中幼年犬(≤6月齡)最為易感,感染率高達到18.6%。同時我們發(fā)現(xiàn)該病毒主要與犬細小病毒(CPV)伴發(fā)感染,其感染率達23.4%。有趣的是,健康犬也可攜帶該病毒,陽性率為5.9%。這說明CAstV普遍存在于寵物犬中,甚至呈隱性感染。本研究根據(jù)部分ORF1b基因構(gòu)建了氨基酸遺傳進化樹,旨在進一步了解CAstV的基因型分布特點,首次在廣西犬群中發(fā)現(xiàn)CAstV可以分為哺乳動物5、3和2型,與它們的氨基酸同源性分別為84.6-100%、97.5-100%和94.9-100%。同時我們根據(jù)部分ORF2基因構(gòu)建了遺傳進化樹,分析發(fā)現(xiàn),所獲得的13株病毒株同屬于哺乳動物5型CAstV譜系,但是被分成了兩個小分支,分別與中國和意大利毒株在一個分支上,與國內(nèi)報道有所不同。此外,本研究成功擴增全長衣殼蛋白(ORF2基因),并根據(jù)對氨基酸序列進行系統(tǒng)分析和構(gòu)建遺傳進化樹。結(jié)果表明ORF2基因全長為2325bp,編碼775個氨基酸。根據(jù)保守性劃分為三個區(qū)域中,以區(qū)域Ⅱ(447aa-730aa)變異較大,除了與2005年意大利毒株和英國毒株(Gillingham/2012/UK)具有較高氨基酸同源性外(95.1-97.6%),其他毒株的同源性相對較低。
[Abstract]:CanineAstrovirus (CAstV) is one of the pathogens causing the disease of canine gastroenteritis. In recent years, the virus has been identified in dogs with intestinal diarrhea. Sequence analysis has found that it belongs to the unique stellate virus species. However, the clinical manifestation and epidemic pattern of the pathogen in pet dogs is not yet clear. Therefore, this study is not clear. To understand the clinical epidemiological characteristics and genotype distribution characteristics of CAstV in pet dogs, to provide a scientific basis for further treatment of the disease and to understand the rule of CAstV epidemic, this study mainly collected 253 fecal samples of pet dogs of different sex and age in 08 months of -2016 in November 2015, using RT- nested PCR (RT-). NPCR) a clinical epidemiological survey of CAstV was conducted. The study found that the positive rate of CAstV detection in dogs with diarrhea was as high as 25.3%, and further confirmed the correlation between diarrhea symptoms and CAstV infection. In addition, the analysis of CAstVs in pet dogs of different varieties, sex and growth stage was analyzed, and the results were found, the pet of different varieties and sexes. The dog can be infected with the virus, of which the young dog (less than 6 month old) is most susceptible and the infection rate is high to 18.6%.. At the same time, we found that the virus is mainly associated with the canine parvovirus (CPV) infection. The infection rate of 23.4%. is interesting that the healthy dog can also carry the virus and the positive rate is 5.9%., which indicates that CAstV is commonly found in pet dogs, even in pet dogs. This study constructed the amino acid genetic evolution tree based on the partial ORF1b gene. The aim of this study was to further understand the genotype distribution characteristics of CAstV. In the first time, the Guangxi canine group found that CAstV could be divided into mammalian 5,3 and type 2, and their amino acids were 84.6-100%, 97.5-100% and 94.9-100%., respectively, according to some O. The RF2 gene constructed the genetic evolution tree. It was found that the 13 strains of the virus were found to belong to the mammalian type 5 CAstV lineage, but were divided into two small branches, which were different from those in one branch of China and Italy, respectively. In addition, this study amplified the full length capsid protein (ORF2 gene) and based on the ammonia. The sequence of basic acid was systematically analyzed and the genetic evolution tree was constructed. The results showed that the total length of ORF2 gene was 2325bp and encoded 775 amino acids. According to conservatism, the gene was divided into three regions, and the variation of the region II (447aa-730aa) was larger, except for the high amino acid homology of the Italy and British strains (Gillingham/2012/UK) in 2005 (95.1-9 7.6%) the homology of other strains is relatively low.
【學(xué)位授予單位】：廣西大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.292

【參考文獻】

相關(guān)期刊論文 前10條

1 徐德順;朱曉娟;陳莉萍;吳曉芳;紀蕾;沈月華;;實時熒光定量RT-PCR快速檢測星狀病毒方法的研究[J];中國衛(wèi)生檢驗雜志;2015年04期

2 蘭家暖;郭旋;劉磊;張民秀;盧冰霞;廖承球;黃福標;黃偉堅;;豬星狀病毒的分離鑒定及其致病性研究[J];中國畜牧獸醫(yī);2012年07期

3 黃雅娟;高愛武;楊金麗;王海榮;侯先志;考桂蘭;苗海明;;綿羊嫩度相關(guān)基因mRNA表達量熒光定量PCR檢測方法的建立[J];食品科技;2012年02期

4 向文龍;宗文萍;胡貴方;;逆轉(zhuǎn)錄環(huán)介導(dǎo)等溫擴增檢測人星狀病毒1型[J];實用醫(yī)學(xué)雜志;2010年20期

5 孫美蓮;王云生;楊冬青;韋朝領(lǐng);高麗萍;夏濤;單育;駱洋;;茶樹實時熒光定量PCR分析中內(nèi)參基因的選擇[J];植物學(xué)報;2010年05期

6 商曉桂;郭偉;楊蓮茹;楊志彪;華修國;朱建國;崔立;;豬星狀病毒SYBR Green Ⅰ實時熒光定量PCR檢測方法的建立與初步應(yīng)用[J];畜牧獸醫(yī)學(xué)報;2010年08期

7 譚冬梅;鄧麗麗;劉巍;;人星狀病毒的研究進展[J];應(yīng)用預(yù)防醫(yī)學(xué);2009年04期

8 磨美蘭;甘書鉆;;動物傳染病研究中PCR引物設(shè)計的技巧及相關(guān)軟件介紹[J];廣西農(nóng)業(yè)生物科學(xué);2008年S1期

9 趙煥英;包金風(fēng);;實時熒光定量PCR技術(shù)的原理及其應(yīng)用研究進展[J];中國組織化學(xué)與細胞化學(xué)雜志;2007年04期

10 何國芳;星狀病毒感染的研究現(xiàn)狀[J];安徽醫(yī)藥;2004年04期

相關(guān)碩士學(xué)位論文 前1條

1 朱愛玲;犬星狀病毒（Canineastrovirus，，CaAstv）半巢式RT-PCR檢測方法的建立及基因序列分析[D];上海交通大學(xué);2012年

本文編號：2027737