本文選題：荷斯坦牛 + 移植��； 參考：《石河子大學(xué)》2017年碩士論文

【摘要】：本研究在生產(chǎn)實(shí)踐基礎(chǔ)上,針對(duì)荷斯坦青年牛作為受體提高胚胎移植受胎率的技術(shù)要點(diǎn),在新疆西部牧業(yè)中心奶牛場(chǎng)和新疆石河子市新安鎮(zhèn)雙鏘牧業(yè)有限責(zé)任公司分別進(jìn)行長效FSH與常規(guī)FSH超排效果的對(duì)比分析、發(fā)情配種后第7天黃體質(zhì)量監(jiān)測(cè)與血清激素的檢測(cè)、胚胎移植主要程序的操作。應(yīng)用直腸檢查和B超對(duì)超排實(shí)驗(yàn)牛和受體牛進(jìn)行跟蹤檢測(cè)和篩查,并對(duì)受體牛實(shí)施移植后優(yōu)化化保胎方案。結(jié)合發(fā)情配種后第7天黃體直徑、周長、面積、體積與血清中E2、LH、P4三種激素水平的變化規(guī)律,為今后進(jìn)一步提高荷斯坦青年牛為受體移植日本黑和牛胚胎受胎率奠定一定基礎(chǔ)。本研究在西部牧業(yè)良繁中心奶牛場(chǎng)選擇18頭淘汰奶牛,其中9頭采用4天8針減量注射法注射短效的FSH,另外9頭一針注射的長效PVP-FSH,通過B超診斷和屠宰后的觀察。超排效果明顯,卵泡上有很多的排卵點(diǎn),結(jié)果表明,短效FSH方案卵泡數(shù)量總計(jì)為179個(gè),平均19.8個(gè);排卵點(diǎn)為82個(gè),平均9.1個(gè);長效PVP-FSH超排方案,卵泡總計(jì)248個(gè),平均為27.5個(gè);排卵點(diǎn)為112個(gè),平均12.4個(gè)。兩者效果差異不顯著(P0.05)。為了研究奶牛發(fā)情后第7天卵巢黃體周長、面積、體積和直徑與血清中激素水平的關(guān)系進(jìn)行研究,隨機(jī)對(duì)85頭奶牛進(jìn)行B超檢查,對(duì)血清中P4、E2和LH用ELISA方法進(jìn)行激素水平檢測(cè)。以便完善在胚胎移植操作程序中對(duì)受體牛選擇。結(jié)果顯示,在檢查的85頭奶牛中有16頭無黃體,把無黃體的奶牛設(shè)為對(duì)照組,把有黃體的奶牛按照不同的指標(biāo)分組分析黃體直徑、面積、周長、體積和血清P4、E2和LH水平的相互關(guān)系。發(fā)情配種后第7天通過B超監(jiān)測(cè)黃體直徑、面積、周長、體積和ELISA血清P4、E2和LH水平的相互關(guān)系。結(jié)果顯示,配種3次以上組與配種3次以下組,黃體直徑、面積、周長、體積和BCS之間差異不顯著(P0.05),但產(chǎn)后天數(shù)之間差異顯著(P0.05);配種1次與配種2次黃體直徑指標(biāo)之間差異顯著(P0.05);體況評(píng)分各組黃體指標(biāo)之間差異均不顯著(P0.05),不同黃體直徑組2.6cmCLD≤3cm組和CLD≥3cm組E2和P4水平差異顯著(P0.05);其它各組之間不同黃體體積、面積和周長指標(biāo)E2、LH、P4水平差異均不顯著(P0.05)。為了獲得更多的純種和牛,選擇199頭牛采用CIDR埋栓做同期發(fā)情,發(fā)情率為96%(191/199);采用臨床觀察、直腸檢查和B超檢查篩選出113頭荷斯坦牛作為受體牛,在發(fā)情后第7天用B型超聲儀對(duì)受體牛黃體直徑進(jìn)行測(cè)量,對(duì)符合條件的受體牛進(jìn)行胚胎移植,將不同黃體直徑分為3個(gè)組,分別為1cm≤CLD≤1.5cm、1.5cmCLD≤2.5cm和CLD2.5cm。在移植后第23天用B超做妊娠診斷,以便研究黃體直徑對(duì)移植受胎率的影響。結(jié)果表明,黃體直徑1cm≤CLD≤1.5cm組、1.5cmCLD≤2.5cm組、CLD2.5cm組受胎率分別是42.86%(6/14)、38.46%(25/65)和44.44%(12/27),各組之間差異均不顯著(P0.05)。
[Abstract]:Based on the production practice, this study aimed at the key points of improving the conception rate of embryo transfer in Holstein young cattle as a receptor. The results of long-term FSH and routine FSH superovulation were compared and analyzed in the dairy farm in the west of Xinjiang and Shuangqiang Animal Husbandry Co., Ltd., Xinan Town, Shihezi City, Xinjiang. The quality of luteal body and serum hormone were monitored 7 days after oestrus mating. The operation of the main procedure for embryo transfer. Rectal examination and B-ultrasound were used to detect and screen the superovulation test and recipient cattle, and the recipient cattle were optimized for fetal preservation after transplantation. The changes of luteal body diameter, circumference, area, volume and serum E2LHHHP4 levels on the 7th day after oestrus mating laid a foundation for further improving the embryo conception rate of Japanese black and black cattle embryos transferred by Holstein young cattle as recipients in the future. In this study, 18 dairy cows were selected for elimination in West China Livestock breeding Center. Among them, 9 cows were injected with short-effect FSHs with 4 days and 8 injections, and 9 cows with long-acting PVP-FSHs were injected with one needle. The results were diagnosed by B-ultrasound and observed after slaughter. The results showed that the total number of follicles was 179 (mean 19.8), the ovulation points were 82 (average 9.1), and the long-acting PVP-FSH superovulation scheme had 248 follicles with an average of 27.5. The number of ovulation points was 112, with an average of 12.4. There was no significant difference in effect between the two groups (P 0.05). In order to study the relationship between the peripheral length, area, volume and diameter of corpus luteum and serum hormone level in 85 cows after estrus 7 days after estrus, 85 cows were examined by B ultrasound, and serum P4E 2 and LH were detected by Elisa. In order to improve the selection of recipient cattle in the procedure of embryo transfer. The results showed that there were 16 lute-free cows in the 85 cows examined, and the luteinless cows were divided into two groups according to different indexes to analyze the diameter, area and circumference of luteal body. The relationship between volume and serum P _ 4 E _ 2 and LH levels. The relationship between the diameter, area, circumference and volume of corpus luteum and the serum levels of P4E _ 2 and LH in Elisa was monitored by B-ultrasound on the 7th day after oestrus mating. The results showed that the diameter, area and circumference of corpus luteum were higher than 3 times and less than 3 times. There was no significant difference between volume and BCS, but there was significant difference between postpartum days (P 0.05), there was significant difference between the two indexes of luteal body diameter (P 0.05), and there was no significant difference in luteal body index among all groups in body condition score (P 0.05), but there was no significant difference among different luteal bodies. There were significant differences in E2 and P4 levels between the diameter group (2.6 cm CLD 鈮，

本文編號(hào)：2010139