發(fā)布時(shí)間：2018-06-07 08:54

  本文選題：綿羊 + 毛色��； 參考：《遺傳》2016年07期

【摘要】：G蛋白偶聯(lián)受體143(G-protein coupled receptor143,GPR143)在黑素體的生物合成中起重要作用,本文旨在研究GPR143基因在不同毛色綿羊皮膚組織中的差異表達(dá)及定位,探索GPR143基因與毛色形成的相關(guān)性。通過q RT-PCR方法和免疫印跡方法分別檢測不同毛色綿羊皮膚組織中GPR143基因m RNA水平和蛋白水平的表達(dá)差異;運(yùn)用免疫熒光法對不同毛色綿羊皮膚組織中的GPR143基因進(jìn)行定位并對結(jié)果進(jìn)行光密度值分析。q RT-PCR結(jié)果顯示,GPR143基因在黑色綿羊皮膚組織中m RNA相對表達(dá)量為白色綿羊的7.84倍,二者差異極顯著(P0.01);免疫印跡結(jié)果顯示,黑色綿羊皮膚組織中GPR143蛋白表達(dá)量是白色綿羊的1.3倍,二者差異顯著(P0.05)。免疫熒光結(jié)果顯示,GPR143蛋白的主要表達(dá)部位為綿羊皮膚組織毛囊外根鞘和表皮層,經(jīng)光密度值分析后發(fā)現(xiàn),GPR143在黑色綿羊皮膚毛囊外根鞘和表皮層的表達(dá)量顯著高于白色綿羊。本研究結(jié)果表明不同毛色綿羊皮膚組織均能表達(dá)GPR143基因,但黑色綿羊皮膚組織中該基因的m RNA和蛋白水平都顯著高于白色綿羊,說明GPR143的m RNA和蛋白在黑色綿羊皮膚組織中表達(dá)上調(diào),在白色綿羊皮膚組織中表達(dá)下調(diào)。GPR143基因可能通過調(diào)控MITF水平和黑素體的數(shù)量、大小、運(yùn)動(dòng)和成熟進(jìn)而參與綿羊毛色的形成過程。
[Abstract]:G protein-coupled receptor 143(G-protein coupled receptor 143 (GPR143) plays an important role in the biosynthesis of melanoma. The purpose of this study was to study the differential expression and localization of GPR143 gene in sheep skin tissues of different wool colors, and to explore the correlation between GPR143 gene and coat color formation. The expression of GPR143 gene m RNA and protein in sheep skin was detected by Q RT-PCR and Western blotting. Using immunofluorescence method, the GPR143 gene in sheep skin of different color was located and the results were analyzed by optical density value. The results showed that the relative expression of m RNA in black sheep skin was 7.84 times as high as that in white sheep. The results of Western blot showed that the expression of GPR143 protein in black sheep skin was 1.3 times higher than that in white sheep, and the difference between them was significant (P 0.05). The expression of GPR143 protein was mainly located in the outer root sheath and epidermis of hair follicles in sheep skin. The expression of GPR143 in the outer root sheath and epidermis of black sheep was significantly higher than that in white sheep by optical density analysis. The results showed that GPR143 gene could be expressed in different skin tissues of sheep, but the level of m RNA and protein in black sheep skin was significantly higher than that in white sheep. The results indicated that the expression of m RNA and protein of GPR143 was up-regulated in black sheep skin, and the down-regulation of .GPR143 gene in white sheep skin might regulate the level of MITF and the quantity and size of melanoma. Exercise and maturation in turn participate in the formation of wool color.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;東北農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;銅仁學(xué)院烏江農(nóng)林經(jīng)濟(jì)學(xué)院;
【基金】：國家高技術(shù)研究發(fā)展計(jì)劃項(xiàng)目(“863”計(jì)劃)(編號(hào):2013AA102506) 國家公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(編號(hào):201303119)資助~~
【分類號(hào)】：S826
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本文編號(hào)：1990600