本文選題：西門塔爾雜種牛 + 生長速度��； 參考：《寧夏大學(xué)》2015年碩士論文

【摘要】：本試驗選取西門塔爾雜種牛243頭為研究對象,選擇5個與生長速度相關(guān)基因,根據(jù)GenBank公布的牛的序列為模板,共設(shè)計了14對引物。利用PCR-SSCP技術(shù)測序分析了5個基因部分序列多態(tài)性,結(jié)果有7對引物(GH-G2、GH-G3、UCP2-U1、UCP2-U2、UCP3-U3、UCP3-U4、NEB-N1)擴增片段存在多態(tài)性,并分析了這4個基因的7個片段多態(tài)性與生長性狀之間的相關(guān)性；利用分子生物學(xué)軟件對基因變異位點進行多態(tài)信息分析。研究結(jié)果如下：1.西門塔爾雜種牛GH-G2、UCP3-U4、NEB-N1位點PIC值分別為0.1376、0.1281、0.2064,處于低度多態(tài)(PIC0.25), GH-G3、UCP2-U1、UCP2-U2、UCP3-U3位點PIC值分別為0.3576、0.4056、0.3491、0.3727,處于中度多態(tài)(0.25PIC0.5)。2.GH-G2片段存在204CT、420GA兩處突變；GH-G3片段存在3699GC、4000GT、4001AG三處突變,其中兩處分別引起亮氨酸變成纈氨酸,纈氨酸變成異亮氨酸；UCP2-U1片段存在632CT、655GA、689CA三處突變,UCP2-U2片段處檢測到643AC的突變,均屬于同義突變；UCP3-U3片段5465AG,引起丙氨酸被蘇氨酸替代；UCP3-U4檢測發(fā)現(xiàn)四處突變分別為7953GC、7921GA、 8018 CT、8162CT; NEB-N1片段20166AG的突變。3.NEB-N1位點適合用于西門塔爾雜種牛增重速度、體高、胸圍分子標(biāo)記輔助選擇,BB型為優(yōu)勢基因型,尤其后期增重效果、胸圍優(yōu)勢非常明顯；UCP3-U3位點對生長后期增重速度影響顯著,可以作為增重分子標(biāo)記輔助選擇；UCP3-U4位點適合用于西門塔爾雜種牛體斜長分子標(biāo)記輔助選擇,AJ3型為優(yōu)勢基因型。
[Abstract]:In this experiment, 243 Simmental hybrid cattle were selected as research objects, 5 genes related to growth rate were selected, and 14 pairs of primers were designed according to the sequence of cattle published by GenBank. The partial polymorphisms of five genes were sequenced by PCR-SSCP technique. The results showed that there were 7 pairs of primers GH-G2HG3UCP2-U1UCP2-U2UCP3-U3C3-UCP3-UCP3-U4NEB-N1). The relationship between the polymorphism of seven fragments of the four genes and the growth traits was analyzed. The polymorphic information of gene mutation sites was analyzed by molecular biology software. The results are as follows: 1. The PIC values of the GH-G2UCP3-U4NB-N1 locus of Simmental hybrid cattle were 0.1376-0.1281nb 0.2064, respectively, and the PIC values of GH-G3UCP2-UCP2-UCP2-UCP2-UCP3-UCP3-U3 were 0.35760.40560.3491N 0.3727, respectively. The mutation GH-G3 at 204CT420GA was 0.35760.40560.3491N 0.3727, and the mutation of GH-G3 at 204CT420GA was 3699GCf4000GT4001AG 3, respectively. The mutation of 643AC was detected at the UCP2-U1 fragment with three mutations of 632CT655GAA689CA and the fragment of UCP2-U2 was changed from valine to isoleucine. All of them belong to the synonymous mutation of UCP3-U3 fragment (5465AGG), which caused alanine to be substituted by threonine for UCP3-U4. The results showed that the four mutations were 7953GCf7921GA and 8018 CT-8162CT.The mutation of NEB-N1 fragment 20166AG. 3. NEB-N1 locus was suitable for Simmental hybrid cattle to gain weight and gain body height. The breast circumference molecular marker-assisted selection of BB genotype was the dominant genotype, especially in the later stage of weight gain. The breast circumference dominance was very obvious. The UCP3-U3 site had a significant effect on the weight gain rate in the late growth period. UCP3-U4 locus can be used as weight gain molecular marker assisted selection of AJ3 genotype in Simmental hybrid cattle.
【學(xué)位授予單位】：寧夏大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S823

【引證文獻】

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1 張爭鋒;陳宏;李秋玲;雷初朝;張春雷;薛愷;王軼敏;王新莊;;南陽牛DGAT2基因多態(tài)性及其與生長性狀相關(guān)性研究[A];第十次全國畜禽遺傳標(biāo)記研討會論文集[C];2006年

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本文編號：1985510