本文選題：基質(zhì)效應(yīng) + 離子抑制率　； 參考：《分析測試學(xué)報》2017年08期

【摘要】：基質(zhì)效應(yīng)普遍存在于液質(zhì)聯(lián)用分析中。該文通過評價"離子抑制率"指標(biāo),系統(tǒng)考察了應(yīng)用超高效液相色譜-電噴霧電離-串聯(lián)質(zhì)譜法(UPLC-ESI-MS/MS)測定豬糞便中二甲氧芐啶、磺胺對甲氧嘧啶、恩諾沙星、環(huán)丙沙星、金霉素、泰樂菌素6種抗生素殘留的基質(zhì)效應(yīng)。樣品經(jīng)McIlvaine-Na_2EDTA緩沖溶液提取后,采用HLB固相萃取柱凈化,濃縮富集后用0.1%甲酸-甲醇溶液(85∶15,體積比)溶解。經(jīng)SB C_(18)RRHD色譜柱分離,以甲醇-0.1%甲酸為流動相進(jìn)行梯度洗脫,在正離子電噴霧下采用多反應(yīng)監(jiān)測模式檢測。結(jié)果表明,豬糞便基質(zhì)對6種抗生素的測定存在基質(zhì)增強效應(yīng)。采用基質(zhì)匹配標(biāo)準(zhǔn)曲線校正法并優(yōu)化樣品處理條件可以對基質(zhì)效應(yīng)產(chǎn)生的影響進(jìn)行補償與消除。該研究為提高檢測結(jié)果的準(zhǔn)確度與精密度提供了借鑒。
[Abstract]:The matrix effect is widely used in liquid-mass spectrometry. The determination of dimethoprim, sulfamethoxine, enrofloxacin, ciprofloxacin, chlortetracycline in pig feces by UPLC-ESI-MS / MSM was systematically studied by evaluating the index of "ion inhibition rate" by ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS). Matrix effect of 6 antibiotic residues of tylosin. The samples were extracted by McIlvaine-Na_2EDTA buffer solution, purified by HLB solid-phase extraction column, concentrated and dissolved in 0.1% formic acid-methanol solution (85: 15, volume ratio). After separation by SB C_(18)RRHD column, gradient elution was carried out with methanol-0.1% formic acid as mobile phase, and detected by multi-reaction monitoring mode under positive ion electrospray. The results showed that porcine fecal matrix had matrix enhancement effect on the determination of 6 antibiotics. The matrix matching standard curve correction method and the optimization of sample treatment conditions can compensate and eliminate the effect of matrix effect. This study provides a reference for improving the accuracy and precision of the detection results.
【作者單位】： 上海市動物疫病預(yù)防控制中心;
【基金】：上海市科技興農(nóng)重點攻關(guān)項目[滬農(nóng)科攻字(2011)第4-9號]資助
【分類號】：O657.63;S859.8

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃寶勇;潘燦平;王一茹;曹U，

本文編號：1982433