本文選題：纖維素酶 + 木聚糖酶�。� 參考：《山西農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本試驗(yàn)采用袋裝黃貯方式,分別添加不同量纖維素酶、木聚糖酶進(jìn)行玉米秸稈黃貯,在10天、30天、50天后開袋測(cè)樣,篩選出纖維素酶、木聚糖酶的最佳添加量,并確定發(fā)酵時(shí)間。然后分四組進(jìn)行黃貯(添加最適纖維素酶組、添加最適木聚糖酶組、混合酶組、對(duì)照組),通過CNCPS體系和DFCCS型雙外流動(dòng)態(tài)瘤胃培養(yǎng)系統(tǒng)評(píng)定不同酶處理的黃貯秸稈對(duì)人工瘤胃及其消化率的影響,選出最佳添加組。試驗(yàn)共分三部分：第一部分：對(duì)玉米秸稈進(jìn)行自然條件下風(fēng)干處理并粉碎,在實(shí)驗(yàn)室條件下進(jìn)行黃貯,分別添加纖維素酶組(添加量為：0,5,10,15,20,25,30ECU/g).木聚糖酶組(添加量為：0,0.05,0.10,0.15,0.20,0.25,0.30IU/g),每個(gè)處理設(shè)3個(gè)重復(fù),常溫下貯存10 d、30d、50d后,開封測(cè)定黃貯的pH、氨態(tài)氮、乳酸、常規(guī)養(yǎng)分等。結(jié)果表明：添加不同水平的纖維素酶、木聚糖酶對(duì)黃貯玉米秸稈的氨態(tài)氮占總氮比值(NH3-N/TN)、中性洗滌纖維(NDF)含量的影響差異顯著(P0.05),pH值隨纖維素酶和木聚糖酶添加量的增加而不斷降低,乳酸含量隨纖維素酶和木聚糖酶添加量的增加而增加,NDF含量呈下降趨勢(shì),且組間差異顯著(P0.05),ADF差異不顯著(P.0.05),綜合感官鑒定和各項(xiàng)指標(biāo),纖維素酶的最佳添加量為20ECU/g (DM),木聚糖組最佳添加量是0.2IU/g (DM),最佳黃貯時(shí)間為30天。第二部分：將黃貯玉米秸稈分為四組,纖維素酶組(20ECU/g)、木聚糖酶組(0.2IU/g)、纖維素酶(20ECU/g)和木聚糖酶(0.2IU/g)混合組、對(duì)照組。常溫下貯存30d后,開封測(cè)樣。用試驗(yàn)室黃貯評(píng)價(jià)法對(duì)其評(píng)定,運(yùn)用CNCPS體系對(duì)四組玉米秸稈進(jìn)行評(píng)價(jià)。結(jié)果表明：各組氨態(tài)氮占總氮比值(NH3-N/TN)、乳酸含量差異顯著(P0.05),pH值差異不顯著(P0.05)。添加混合酶組的LA、CP、PB1、CB1、CC含量均最高,其次為纖維素酶組、木聚糖酶組,對(duì)照組含量最低。綜合各項(xiàng)指標(biāo),黃貯效果最好的為混合酶組,然后是纖維素酶組、木聚糖酶組,最后是對(duì)照組。第三部分：將試驗(yàn)二不同組玉米秸稈樣品和精料制粒,分為A組(纖維素酶秸稈制粒組)、B組(木聚糖酶秸稈制粒組)、AB組(混合酶秸稈制粒組)和C組(對(duì)照組)。利用DFCCS型雙外流動(dòng)態(tài)瘤胃培養(yǎng)系統(tǒng),對(duì)A、B、AB、C四組進(jìn)行仿牛瘤胃發(fā)酵處理試驗(yàn),評(píng)價(jià)其對(duì)人工瘤胃發(fā)酵的影響。結(jié)果表明：四組中的pH值變化不顯著(P0.05), A, B, AB組的表觀消化率和總酸均比對(duì)照組均高,且組間差異顯著(P0.05),AB組最高,A組次之,B組最低。
[Abstract]:In this experiment, different cellulase and xylanase were added to yellow silage by adding different amounts of cellulase and xylanase. The optimum adding amount of cellulase, xylanase and fermentation time were screened at 10 days, 30 days and 50 days later. Then four groups were added to the Yellow silase group (adding the optimum cellulase group and adding the optimum xylanase group). The effects of different enzyme treated yellow silage straw on the artificial rumen and its digestibility were evaluated by CNCPS system and DFCCS dual flow dynamic rumen culture system. The best addition group was selected. The experiment was divided into three parts: the first part: the wind dry treatment and crushing of corn stalk under natural conditions and the laboratory conditions. Under the Yellow silage, the cellulase group was added (adding amount of 0,5,10,15,20,25,30ECU/g). The xylanase group (adding amount was 0,0.05,0.10,0.15,0.20,0.25,0.30IU/g), each treatment was set up 3 repetitions, and stored at 10 d at normal temperature, 30d, and 50D, the pH, ammonia nitrogen, lactic acid and conventional nutrients were determined by Kaifeng, and the results showed that the addition of different levels was added. The effect of Xylanase on the ratio of ammonia nitrogen to total nitrogen (NH3-N/TN) and the content of neutral detergent fiber (NDF) was significantly different (P0.05). The pH value decreased with the increase of cellulase and xylanase, and the content of lactic acid increased with the increase of fibrin and xylanase, and the content of NDF was lower. The difference was significant (P0.05), and the difference of ADF was not significant (P.0.05). The optimum addition amount of cellulase was 20ECU/g (DM), the optimum addition of xylan group was 0.2IU/g (DM) and the best yellow storage time was 30 days. Second parts: the Yellow silage corn straw was divided into four groups, cellulase group (20ECU/g), xylan Enzyme group (0.2IU/g), cellulase (20ECU/g) and xylanase (0.2IU/g) mixed group, control group. After storage of 30d at normal temperature, Kaifeng sample was measured. Four groups of corn stalks were evaluated by test room yellow storage evaluation method and CNCPS system was used to evaluate the ratio of ammonia nitrogen to total nitrogen ratio (NH3-N/TN), and the difference of lactic acid content was significant (P0.05), pH The value difference was not significant (P0.05). The content of LA, CP, PB1, CB1 and CC in the mixed enzyme group was the highest, followed by cellulase group, xylanase group, and the control group with the lowest content. The comprehensive index, the best yellow storage effect was the mixed enzyme group, and then the cellulase group, the xylanase group and the control group. The third part: the experiment two different groups of jade will be tested. Rice straw samples and fine grain granulation were divided into A group (cellulase straw granulating group), B group (xylanase straw granulation group), AB group (mixed enzyme straw granulation group) and C group (control group). The fermentation process of A, B, AB and C four groups was tested by DFCCS double flow dynamic rumen culture system, and the effects on Rumen Fermentation of artificial rumen were evaluated. The results showed that the change of pH value in the four groups was not significant (P0.05). The apparent digestibility and total acid in the group of A, B and AB were all higher than those of the control group, and the difference between the groups was significant (P0.05), the AB group was the highest, the A group was the next, and the B group was the lowest.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S816

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