本文選題：枯草芽孢桿菌 + AGP��； 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：枯草芽孢桿菌和抗生素類生長促進劑(Antibiotic Growth Promoters,AGP)主要通過調(diào)控腸道健康促進畜禽生長。本試驗以0-6周齡蛋雞為研究對象,通過雙因素析因試驗設(shè)計,分析枯草芽孢桿菌和AGP對蛋雞機體生長和腸道功能的作用,并嘗試通過腸道結(jié)構(gòu)和功能的相關(guān)指標(biāo)對兩者的益生機制和互作效應(yīng)進行分析,為枯草芽孢桿菌和AGP在家禽生產(chǎn)上更科學(xué)高效的應(yīng)用提供理論依據(jù)。試驗一枯草芽孢桿菌和AGP對0-6周齡蛋雞生長發(fā)育的影響本試驗通過分析枯草芽孢桿菌和AGP對0-6周齡蛋雞生長發(fā)育的影響及其互作效應(yīng),以期對枯草芽孢桿菌和AGP對蛋雞影響的作用機制的異同進行研究,為益生菌在含AGP日糧中的應(yīng)用方式提供理論依據(jù)。試驗選取1日齡海蘭褐蛋雞504只,隨機分為4個處理組,每組7個重復(fù),每個重復(fù)18只雞。試驗處理如下:對照組:飼喂基礎(chǔ)日糧;AGP組:基礎(chǔ)日糧添加20 mg/kg桿菌肽鋅和4 mg/kg硫酸粘桿菌素;枯草芽孢桿菌組:基礎(chǔ)日糧添加500mg/kg枯草芽孢桿菌制劑;枯草芽孢桿菌+抗生素組:基礎(chǔ)日糧添加500mg/kg枯草芽孢桿菌制劑、20 mg/kg桿菌肽鋅和4 mg/kg。結(jié)果表明:枯草芽孢桿菌對蛋雞0-3周齡的生長性能指標(biāo)均無顯著影響,但能夠顯著降低蛋雞的4-6周的料重比(P0.05);枯草芽孢桿菌和AGP對蛋雞第3和第6周的免疫器官指數(shù)均無顯著影響(P0.05),但兩者在3周齡蛋雞的脾臟指數(shù)上存在顯著的拮抗作用(P0.05);枯草芽孢桿菌和AGP對第3周齡和第6周齡蛋雞的各消化器官指數(shù)和體重均勻度均無顯著影響(P0.05),且兩者不存在顯著交互作用(P0.05);因此,從本試驗所選取的指標(biāo)來看,日糧添加枯草芽孢桿菌和AGP對蛋雞0-3周齡的生長發(fā)育無顯著影響;日糧添加枯草芽孢桿菌對蛋雞4-6周齡的生長發(fā)育的促進效果優(yōu)于AGP。試驗二枯草芽孢桿菌和AGP對0-6周齡蛋雞腸道功能的影響本試驗旨在通過小腸形態(tài)結(jié)構(gòu)、小腸消化酶基因表達、小腸屏障作用蛋白表達及小腸SIgA濃度等指標(biāo),研究枯草芽孢桿菌和AGP對0-6周齡蛋雞腸道健康的影響。試驗設(shè)計同試驗一。結(jié)果表明,枯草芽孢桿菌能夠降低3周齡蛋雞十二指腸和空腸隱窩深度,提高6周齡空腸絨毛高度和3周齡絨毛高度/隱窩深度值;AGP可顯著提高3周齡回腸絨毛高度/隱窩深度值(P0.05);兩者在小腸形態(tài)結(jié)構(gòu)上未見互作效應(yīng)(P0.05);枯草芽孢桿菌能夠顯著提高3周齡十二指腸蔗糖酶和6周齡空腸中的蔗糖酶基因表達,但對于3周齡回腸氨肽酶基因表達具有顯著降低作用(P0.05);AGP可顯著降低3周齡十二指腸蔗糖酶基因表達,并使3周齡回腸麥芽糖酶和氨肽酶,6周齡回腸麥芽糖酶和蔗糖酶基因表達顯著提高(P0.05);兩者在3周齡的十二指腸蔗糖酶和空腸麥芽糖酶上存在拮抗作用(P0.05);枯草芽孢桿菌和AGP對0-6周齡蛋雞小腸屏障作用蛋白和SIgA濃度無顯著影響,也不存在互作效應(yīng)(P0.05)。因此,枯草芽孢桿菌能改善0-6周齡蛋雞十二指腸和空腸形態(tài)結(jié)構(gòu),提高十二指腸和空腸二糖酶基因表達;AGP能改善回腸形態(tài)結(jié)構(gòu)并提高回腸消化酶表達水平。綜上所述,日糧添加枯草芽孢桿菌和AGP對蛋雞0-3周齡的生長發(fā)育無顯著影響,枯草芽孢桿菌對蛋雞4-6周齡的生長發(fā)育的促進效果優(yōu)于AGP;枯草芽孢桿菌能改善0-6周齡蛋雞十二指腸和空腸形態(tài)結(jié)構(gòu)并調(diào)控其消化酶基因表達,AGP則主要通過改善回腸形態(tài)結(jié)構(gòu)并提高消化酶基因表達發(fā)揮作用。在3周齡重點關(guān)注的腸道發(fā)育和生長性能上及6周齡所有指標(biāo)上,枯草芽孢桿菌和AGP均不存在拮抗作用。
[Abstract]:Bacillus subtilis and antibiotic growth promoter (Antibiotic Growth Promoters, AGP) mainly regulate the growth of livestock and poultry by regulating intestinal health. In this experiment, 0-6 weeks old laying hens were used as the research object. The effects of Bacillus subtilis and AGP on the growth and intestinal function of egg chickens were analyzed by the dual factorial design. The probiotic mechanism and interaction effect of the intestinal structure and function were analyzed to provide a theoretical basis for the more scientific and efficient application of Bacillus subtilis and AGP in poultry production. The test of Bacillus subtilis and AGP on the growth and development of 0-6 weeks old laying hens through the analysis of Bacillus subtilis and AGP to 0-6 The effects of the growth and development of the week old laying hens were studied in order to study the differences and similarities of the effects of Bacillus subtilis and AGP on the laying hens, and to provide a theoretical basis for the application of probiotics in the diet containing AGP. The 1 day old hay Brown laying hens were selected and divided into 4 treatment groups randomly, with 7 repetitions in each group, each repetition 18. Only chickens were treated as follows: control group: feeding base diet; group AGP: basal diet supplemented with 20 mg/kg bacilli and 4 mg/kg Mycobacterium; Bacillus subtilis group: basal diet added 500mg/kg Bacillus subtilis preparation; Bacillus subtilis + antibiotic group: basal diet added 500mg/kg Bacillus subtilis preparation, 20 mg/kg bacilli The results of peptide zinc and 4 mg/kg. showed that Bacillus subtilis had no significant effect on the growth performance indexes of 0-3 weeks of laying hens, but could significantly reduce the 4-6 weeks' feed weight ratio (P0.05). Bacillus subtilis and AGP had no significant effect on the third and sixth weeks' immune organ index (P0.05), but both were on the spleen index of 3 week old laying hens. There was significant antagonism (P0.05); Bacillus subtilis and AGP had no significant influence on the digestive organs index and weight uniformity of third week and sixth week old layers (P0.05), and there was no significant interaction between them (P0.05). Therefore, from the index selected in this experiment, the diet added Bacillus subtilis and AGP for 0-3 weeks of laying hens The growth and development of age were not significantly affected, and the effect of Bacillus subtilis on growth and development of 4-6 weeks old was better than that of AGP. test two Bacillus subtilis and the effect of AGP on intestinal function of 0-6 weeks old laying hens. The experiment was aimed at the small intestine morphologic structure, the gene expression of intestinal digestive enzyme, the expression of small intestinal barrier protein and the small intestine. The effects of Bacillus subtilis and AGP on intestinal health of 0-6 week old laying hens were studied. Experimental design and Experiment 1. The results showed that Bacillus subtilis could reduce the depth of duodenum and jejunum recess in 3 weeks old laying hens, increase the height of jejunum villus and the depth of the villus height / recess depth at the age of 6 weeks of age, and the AGP could significantly increase 3 of the 6 weeks of age. The height of the chorionic villus / recess depth (P0.05) at the week age (P0.05), and Bacillus subtilis could significantly increase the expression of invertase gene in duodenal invertase and 6 week old jejunum at 3 weeks of age, but significantly decreased the expression of the gene expression of ileum aminopeptidase at the age of 3 weeks (P0.05); AGP could be significant. The expression of the duodenal invertase gene was reduced at 3 weeks, and the expression of the ileum maltase and aminopeptidase at the age of 3 weeks was significantly increased (P0.05), and the antagonism of the both was antagonistic on the duodenal invertase and jejunum maltase at the age of 3 weeks (P0.05), and Bacillus subtilis and AGP on the small intestine of 0-6 week old laying hens There was no significant effect of barrier effect protein and SIgA concentration and no interaction effect (P0.05). Therefore, Bacillus subtilis can improve the morphological structure of duodenum and jejunum in 0-6 weeks old laying hens and increase the expression of two enzyme gene in duodenum and jejunum; AGP can improve the morphological structure of the ileum and improve the expression level of ileum digestive enzyme. Bacillus subtilis and AGP had no significant effect on the growth and development of 0-3 weeks of laying hens. Bacillus subtilis promoted the growth and development of 4-6 weeks of laying hens better than that of AGP; Bacillus subtilis could improve the morphological structure of duodenum and jejunum in 0-6 weeks old laying hens and regulate the gene expression of digestive enzymes. AGP mainly improved ileum form. There was no antagonism between Bacillus subtilis and AGP on all indexes of intestinal development and growth performance and 6 weeks of age at the age of 3 weeks of age.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S831.5

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