發(fā)布時(shí)間：2018-05-29 03:13

  本文選題：放牧強(qiáng)度 + 根際��； 參考：《草地學(xué)報(bào)》2016年05期

【摘要】：利用涂布平板法和變性梯度凝膠電泳(denatured gradient gel electrophoresis,DGGE)技術(shù),檢測(cè)4個(gè)放牧強(qiáng)度(對(duì)照、輕度放牧、中度放牧和重度放牧)下,大針茅(Stipagrandis)植物根際和非根際土壤中細(xì)菌數(shù)量和群落特征的變化。結(jié)果表明,放牧對(duì)細(xì)菌群落多樣性及組成有顯著影響,但對(duì)細(xì)菌數(shù)量沒有明顯影響。隨著放牧強(qiáng)度的增加,根際和非根際土壤細(xì)菌數(shù)量及群落多樣性的變化趨勢(shì)不同。根際土壤中,在中度放牧強(qiáng)度下,細(xì)菌數(shù)量最高,輕度放牧強(qiáng)度下,細(xì)菌多樣性最高。說(shuō)明細(xì)菌群落多樣性比細(xì)菌數(shù)量敏感。而在非根際土壤,細(xì)菌數(shù)量和群落多樣性隨著放牧強(qiáng)度的增加而逐漸降低。說(shuō)明根際細(xì)菌較非根際細(xì)菌群落有更好的抗干擾能力。同一放牧強(qiáng)度下,根際土壤細(xì)菌數(shù)量及群落多樣性高于非根際土壤,但根際和非根際土壤細(xì)菌群落結(jié)構(gòu)差異較小�？傊�,與非根際細(xì)菌相比,根際細(xì)菌群落具有更好的抗干擾能力,及其與非根際土壤細(xì)菌群落組成的相似性說(shuō)明在生態(tài)恢復(fù)過程中,根際細(xì)菌群落可能作為一個(gè)菌種庫(kù)存在。
[Abstract]:Four grazing intensities (light grazing, moderate grazing and heavy grazing) were measured by using the coated plate method and denatured gradient gel electrophoresistics (DGGE) technique. Changes in the number and community characteristics of bacteria in rhizosphere and non-rhizosphere soils of Stipagrandises. The results showed that grazing had a significant effect on the diversity and composition of bacterial community, but not on the number of bacteria. With the increase of grazing intensity, the number of bacteria and the diversity of community in rhizosphere and non-rhizosphere soil were different. In rhizosphere soil, the number of bacteria was the highest under moderate grazing intensity, and the diversity of bacteria was highest under light grazing intensity. It shows that the diversity of bacterial community is more sensitive than the number of bacteria. In non-rhizosphere soil, the number of bacteria and community diversity decreased with the increase of grazing intensity. The results showed that rhizosphere bacteria had better anti-interference ability than non-rhizosphere bacteria community. Under the same grazing intensity, the number and community diversity of bacteria in rhizosphere soil was higher than that in non-rhizosphere soil, but there was little difference in bacterial community structure between rhizosphere and non-rhizosphere soil. In a word, compared with non-rhizosphere bacteria, rhizosphere bacterial community has better anti-interference ability, and its similarity with non-rhizosphere soil bacterial community indicates that the rhizosphere bacterial community may be used as a stock of bacteria in the process of ecological restoration.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院植物生物技術(shù)功能實(shí)驗(yàn)室;內(nèi)蒙古農(nóng)業(yè)大學(xué)生態(tài)環(huán)境學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31360113) 內(nèi)蒙古自然科學(xué)基金(2015MS0305)資助
【分類號(hào)】：S812.2

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