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  本文選題：蛋雞腸道健康 + 飼養(yǎng)密度�。� 參考：《黑龍江八一農(nóng)墾大學(xué)》2015年碩士論文

【摘要】：為了探索飼養(yǎng)密度對蛋雞腸道健康的影響，本實驗通過設(shè)計不同的飼養(yǎng)密度，比較蛋雞小腸腸道黏膜組織形態(tài)和盲腸腸道內(nèi)細菌菌群區(qū)系，評價蛋雞的健康情況，以選出利于蛋雞健康的籠養(yǎng)密度，從而指導(dǎo)生產(chǎn)。隨機選取1250只11周齡海蘭灰（Hy-Line Gray）蛋雞，按照不同飼養(yǎng)密度隨機分為5個處理組：A組900cm2/只、B組675cm2/只、C組540cm2/只、D組450cm2/只、E組380cm2/只，每個處理內(nèi)50個重復(fù)。5個處理組均在相同的環(huán)境和飼養(yǎng)管理模式下進行飼養(yǎng)。分別于16周齡、26周齡、50周齡時，在每個處理中隨機選取5只雞，取十二指腸、空腸、回腸腸道樣本，測定其絨毛高度和隱窩深度，并計算兩者比值；取盲腸內(nèi)容物，用PCR-DGGE法進行腸道菌群分析。 結(jié)果表明：十二指腸、空腸、回腸絨毛高度與隱窩深度的比值均在26周齡時較高，飼養(yǎng)密度為675cm2/只時十二指腸、空腸絨毛高度與隱窩深度的比值較高，飼養(yǎng)密度為675cm2/只和450cm2/只時回腸絨毛高度與隱窩深度的比值較高；飼養(yǎng)時間和飼養(yǎng)密度對蛋雞盲腸腸道菌群均有著明顯的影響，其中，26周齡時，各密度條件飼養(yǎng)下的蛋雞盲腸腸道菌群差異較小，50周齡時，各密度條件飼養(yǎng)下的蛋雞盲腸腸道菌群差異較大。盲腸腸道菌群主要包括擬桿菌門、厚壁菌門、軟壁菌門、放線菌門、蛋白菌門、酸桿菌門。 綜上所述，飼養(yǎng)時間和飼養(yǎng)密度對蛋雞盲腸腸道菌群均有著明顯的影響，，且飼養(yǎng)密度為675cm2/只時對小腸腸道形態(tài)的生長發(fā)育比較有利，有助于提高小腸的消化吸收功能。
[Abstract]:In order to explore the effect of feeding density on the intestinal health of laying hens, the intestinal mucosa morphology and bacterial flora in the caecal intestine of laying hens were compared by designing different feeding densities to evaluate the health status of laying hens. In order to select the healthy cage density for laying hens, thus guiding production. 1250 11-week-old Hy-Line Hy-Line laying hens were randomly divided into five groups according to different feeding densities: five groups: group A, 900cm2/, group B, 675cm2/, group C, 540cm2/, group D, 450cm2/, group E, 380cm2/. There were 50 duplicates in each treatment. 5 groups were fed in the same environment and management mode. At the age of 16 weeks and 26 weeks and 50 weeks of age, 5 chickens were randomly selected in each treatment. The villus height and crypt depth were measured, and the contents of cecum were taken from the duodenum, jejunum and ileum. The intestinal flora was analyzed by PCR-DGGE method. The results showed that the ratio of villi height to crypt depth in duodenum, jejunum, ileum and ileum was higher at 26 weeks of age. The ratio of villi height to crypt depth was higher when feeding density was 675cm2/. The ratio of the height of ileal villi to the depth of crypt was higher when the feeding density was 675cm2/ and 450cm2/, and the feeding time and density had a significant effect on the intestinal flora of the caecum of laying hens, especially at the age of 26 weeks. The difference of intestinal flora of caecum of layers under different density conditions was smaller than that under different density conditions at the age of 50 weeks, and the difference of intestinal flora of caecum of laying hens under different density conditions was greater than that under different density conditions. The intestinal flora of the cecum mainly includes Bacteroides phylum, Thinacea, Softwort, Actinomycetes, Proteinomycetes, Acidobacterium. In conclusion, the feeding time and density had a significant effect on the intestinal flora of layer hens, and the feeding density of 675cm2/ was beneficial to the growth and development of intestinal morphology, which was helpful to improve the digestion and absorption function of the small intestine.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S831.4

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