本文選題：尾分析法 + 全基因組關聯分析　； 參考：《畜牧獸醫(yī)學報》2017年07期

【摘要】：旨在將尾分析法與全基因組關聯分析相結合,初步確定兩尾選擇的標準。首先,對樣本量為2 000、1 500、1 000、500的群體進行全基因組關聯分析(GWAS),探索群體規(guī)模大小對GWAS結果的影響,然后對各樣本量兩尾的20%、15%、12%、10%、8%、5%進行GWAS,探索兩尾比例的大小對GWAS結果的影響。研究結果表明,檢測出的顯著關聯SNP數目隨群體規(guī)模及兩尾比例的減小而減少。初步確定兩尾選擇標準為:遺傳力為0.38左右的性狀,樣本量為2 000、1 500、1 000、500,兩尾選擇比例分別為8%、10%、10%、20%;遺傳力為0.50左右的性狀,樣本量為2 000、1 500、1 000、500,兩尾選擇比例分別為5%、5%、10%、15%。以此標準為基礎進行GWAS,既能有效檢測出最顯著SNP位點或區(qū)間,又能最大程度地降低研究成本,提高研究效率。
[Abstract]:The purpose of this study was to combine tail analysis with genomic association analysis to determine the criteria for selection of two tails. Firstly, the whole genome association analysis was carried out on a population with a sample size of 2 000 / 1 500 / 1 000500 to explore the effect of population size on the results of GWAS, and then GWASS was carried out on 20 / 15 / 12 / 1088% of the two samples to explore the effect of the size of the two tails on the results of GWAS. The results show that the number of significant association SNP detected decreases with the decrease of population size and two-tail ratio. The selection criteria of two tails were as follows: the heritability was about 0.38, the sample size was 2 000 / 1 500, the ratio of the two selection was 8 / 10 / 10 / 10 / 20, the sample size of the traits with heritability of about 0.50 was 2 000 / 1 500 / 100 / 500, and the ratio of the two selections was 5 / 10 / 10 / 1 / 500 and the ratio of the two selections was 5 / 10 / 10 / 15 / 500, respectively, in which the heritability was about 0. 50 and the sample size was 2 000 / 1 / 100 / 100 / 500, respectively. On the basis of this standard, GWASs can be used to detect the most significant SNP loci or regions, reduce the research cost and improve the research efficiency.
【作者單位】： 湖南農業(yè)大學動物科學技術學院;中國農業(yè)科學院北京畜牧獸醫(yī)研究所;湖南省畜禽安全生產協同創(chuàng)新中心;
【基金】：國家科技支撐計劃項目(2015BAD03B02-2) 中國農業(yè)科學院科技創(chuàng)新工程(ASTIP-IAS02) 國家生豬產業(yè)技術體系(CARS-36)
【分類號】：S813.1
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本文編號：1936853