本文選題：宮內(nèi)胎兒生長受限 + 胎兒　； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本實(shí)驗(yàn)研究了蒙古綿羊妊娠后期營養(yǎng)限飼引起胎兒IUGR,對胎兒腎臟生長發(fā)育的影響。選擇體況中等、2-3胎次,同期發(fā)情及受孕的健康蒙古綿羊24只,妊娠期90d時(shí),選出6只母羊開始屠宰實(shí)驗(yàn),其余按體重任意分配到能量水平依次降低的3組,其中每組6只。飼喂在不同的能量飼養(yǎng)水平：CG組(自由采食,0.67MJME·BW-0.75·d-1);RG2組(0.33 MJME·BW-0.75·d-1);RG1組(0.175MJME·BW-0.75·d-1),至妊娠140d時(shí)對全部實(shí)驗(yàn)組母羊進(jìn)行屠宰。實(shí)驗(yàn)結(jié)果表明：(1)妊娠后期隨著營養(yǎng)限制程度加大,RG1組(P0.01)和RG2(P0.05)胎兒腎臟重和腎臟生長速率明顯降低；RG2、RGl組(P0.05)胎兒腎臟干物質(zhì)重和脂肪重顯著降低；RG1組水分重和蛋白質(zhì)重顯著降低(P0.01)；RG2(p0.05)、RG1組(p0.01)灰分重低于CG組。(2)妊娠后期隨著營養(yǎng)限制程度加大,單位視野內(nèi),RGl組腎小球的數(shù)目多于RG2組(P0.05),系膜輕度增生,增加系膜細(xì)胞數(shù)量,毛細(xì)血管袢明顯增厚；Masson染色膠原蛋白纖維化程度呈加深趨勢,RG1組I膠原蛋白濃度極顯著低于CG組(P0.01)。(3)妊娠后期隨著營養(yǎng)限制程度加大,RG1、RG2組胎兒腎臟組織中總DNA含量顯著低于CG組(P0.01)。(4)妊娠后期隨著營養(yǎng)限制程度加大,RG1胎兒腎臟T-AOC低于CG組(P0.05組；RGl組胎兒腎臟中SOD活性顯著低于CG組(P0.05)；RG1組胎兒腎臟中GSH-Px活性顯著低于RG2(P0.05)、CG組(P0.01)：RG1組胎兒腎臟中MDA的濃度均顯著高于RG2、CG組(P0.01)；RG1組抑制羥自由基能力高于CG組(P0.01)；RG1(P0.01)、RG2組(P0.05)胎兒腎臟黃嘌呤氧化酶活力低于CG組；RG1組胎兒腎臟過氧化物酶活力低于CG組(P0.01)(5)妊娠后期隨著營養(yǎng)限制程度加大,RG1胎兒腎臟Scr和BUN低于CG、RG2組(P0.01)；RG1組胎兒腎臟中β2-MG顯著高于CG、RG2組組(P0.05)；RG1組胎兒腎臟中Cys C顯著高于于CG組(P0.01)。
[Abstract]:The effects of feeding restriction on fetal kidney growth in Mongolian sheep were studied. At 90 days gestation, 6 ewes were selected to begin slaughtering experiment, and the others were divided into 3 groups according to their weight and energy level, among which 6 were in each group. All the ewe of experimental group were slaughtered at different energy levels (0.67 MJME BW-0.75 d-1 + RG2 0.33 MJME BW-0.75 d-1) and 0.175 MJME BW-0.75 d-1 at day 140 of gestation. The results showed that the fetal kidney weight and kidney growth rate decreased significantly in the late pregnancy with the increase of nutritional restriction (P0.01) and RG2P0.05) the weight of dry matter and fat of fetal kidney decreased significantly in RG1 group and protein in RG1 group. The ash content in RG1 group was significantly lower than that in CG group (P 0.01). The number of glomeruli in the RGl group was more than that in the RG2 group (P 0.05), and the Mesangial cells were slightly proliferated and the number of Mesangial cells was increased. The degree of collagen fibrosis in RG1 group was significantly lower than that in CG group (P 0.01. 01. 3) the total DNA content in renal tissue of RG1RG2 group increased with the increase of nutritional restriction in the latter trimester of pregnancy. Lower than CG group (P0.01P0.01P0.01P0.01P0.01, P < 0.05) the SOD activity of RG1 fetal kidney in late pregnancy was lower than that of CG group (P 0.05). The SOD activity of RG1 group was significantly lower than that of CG group (P0.05 + RG1 group was lower than that of CG group P0.01 + RG1 group.) the fetal kidney SOD activity in RG1 group was significantly lower than that in CG group (P0.01 + RG1 group). The concentrations of MDA in the viscera were significantly higher than those in RG2CG-P0.01P0.01RG1 group. The ability of inhibiting hydroxyl free radicals in RG1 group was higher than that in CG group. The activity of xanthine oxidase in fetal kidney was lower than that in CG group. The activity of renal peroxidase in RG1 group was lower than that in CG group. The levels of Scr and BUN in RG1 fetal kidney were significantly higher than those in CG group (P 0.01). The level of 尾 2-MG in RG1 group was significantly higher than that in CG group (P 0.05) and that in RG 1 group was significantly higher than that in CG group (P 0.01).
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S826

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本文編號：1934357