本文選題：凝結(jié)芽孢桿菌 + 分離　； 參考：《中國(guó)畜牧獸醫(yī)》2017年04期

【摘要】：試驗(yàn)旨在分離鑒定一株來(lái)源于池塘底泥的具有益生功能的凝結(jié)芽孢桿菌,并研究其益生性能。從自然池塘底泥分離得到一株產(chǎn)酸能力強(qiáng)的桿狀細(xì)菌,通過(guò)生理生化特征分析和16SrDNA序列比對(duì),鑒定分離菌為凝結(jié)芽孢桿菌(Bacillus coagulans)。該分離菌株對(duì)大腸桿菌K88、大腸桿菌K99、無(wú)乳鏈球菌和嗜水氣單胞菌有抑制作用;在pH 2.5的模擬胃液中處理12h存活率為71.9%;在膽鹽濃度為0.3%的模擬腸液中處理12h,存活率為84.4%。該分離菌株可有效降解亞硝酸鹽,48h降解率達(dá)到96.80%。將270只1日齡AA肉雞分成3個(gè)處理組,日糧分別添加0(Ⅰ組)、1.0×10~6(Ⅱ組)和1.0×10~7 CFU/g(Ⅲ組)劑量的凝結(jié)芽孢桿菌NJ102,試驗(yàn)期42d。結(jié)果顯示,Ⅱ、Ⅲ組肉雞的平均日增重和終末體重顯著高于Ⅰ組(P0.05),料重比顯著低于Ⅰ組(P0.05),各組間平均日采食量差異均不顯著(P0.05)。結(jié)果表明,來(lái)源于池塘底泥的凝結(jié)芽孢桿菌NJ102有一定的抗逆性和益生能力,能改善肉雞的生長(zhǎng)性能,具備作為微生物飼料添加劑開(kāi)發(fā)的潛力。
[Abstract]:The purpose of this study was to isolate and identify a probiotic Bacillus coagulans from pond sediment and to study its probiotic properties. A rod-like bacterium with strong acid-producing ability was isolated from the sediment of natural pond. The bacteria was identified as Bacillus coagulansa by physiological and biochemical analysis and 16SrDNA sequence alignment. The strain had inhibitory effect on Escherichia coli K88, Escherichia coli K99, Streptococcus hydrophila and Aeromonas hydrophila, and the survival rate was 71.9 in simulated gastric juice at pH 2.5 and 84.4in simulated intestinal fluid containing 0.3% bile salt for 12 hours. The strain could effectively degrade nitrite in 48 h and the degradation rate was 96.80%. 270 1-day-old AA broilers were divided into three treatment groups and fed diets supplemented with 0 (group 鈪，

本文編號(hào)：1929545