發(fā)布時(shí)間：2018-05-23 11:54

  本文選題：馬 + 蹄葉炎��； 參考：《東北農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：運(yùn)動(dòng)系統(tǒng)疾病是對(duì)馬屬動(dòng)物危害極大的一類疾病,蹄葉炎又是運(yùn)動(dòng)系統(tǒng)疾病中最常見的疾病�；诖�,本實(shí)驗(yàn)研究馬低聚果糖(OF)急性蹄葉炎模型血液循環(huán)中脂多糖(LPS)、脂聯(lián)素(ADPN)、胰島素、血糖、白細(xì)胞介素-6(IL-6)、p38絲裂原活化蛋白激酶(p38MAPK)、5-羥色胺(5-HT)、內(nèi)皮素-1(ET-1)、血栓素B2(TXB2)和一氧化氮(NO)的濃度變化趨勢(shì)和蹄部的病理變化,探尋蹄葉炎的發(fā)病機(jī)制,尋找可以作為早期快速診斷的生物指標(biāo),并為蹄葉炎綜合預(yù)防與治療提供理論基礎(chǔ)。將21匹蒙古馬隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組。實(shí)驗(yàn)組馬匹灌服10 g/kg體重的OF,對(duì)照組馬匹灌服蒸餾水。每隔4 h采集血液樣本一次,直至72 h。實(shí)驗(yàn)使用Obel分級(jí)標(biāo)準(zhǔn)對(duì)跛行程度進(jìn)行評(píng)估,將一匹跛行達(dá)到3級(jí)的馬進(jìn)行安樂死,沿蹄部矢狀面剖開,去掉第三趾骨,取出蹄葉層做HE染色和PAS染色。實(shí)驗(yàn)結(jié)果表明,實(shí)驗(yàn)組中有11匹馬表現(xiàn)出體溫升高,呼吸、心率加快、跛行、腹瀉等臨床癥狀,實(shí)驗(yàn)組出現(xiàn)臨床癥狀的11匹馬在灌服OF后8-20 h陸續(xù)出現(xiàn)一級(jí)跛行,24-56 h陸續(xù)出現(xiàn)二級(jí)跛行。隨著二級(jí)跛行的出現(xiàn),馬匹呼吸率和心率增加,糞便p H值在20 h下降到最低值,直腸溫度在40 h達(dá)到峰值。所有實(shí)驗(yàn)組馬匹在12-24 h期間都發(fā)生腹瀉,所有臨床癥狀均在60 h后逐漸減輕。對(duì)照組的馬均未出現(xiàn)體溫升高,呼吸、心率加快、跛行、腹瀉等臨床癥狀。X光檢查發(fā)現(xiàn)實(shí)驗(yàn)組馬匹第三指骨發(fā)生旋轉(zhuǎn),對(duì)照組馬匹第三指骨未見旋轉(zhuǎn)。HE及PAS染色發(fā)現(xiàn)實(shí)驗(yàn)組馬匹蹄葉層出現(xiàn)炎性細(xì)胞浸潤(rùn)、嗜堿性染料聚集在血管周圍、細(xì)胞變形等組織學(xué)變化,對(duì)照組馬匹蹄葉層未見組織學(xué)變化。實(shí)驗(yàn)組表現(xiàn)出現(xiàn)臨床癥狀的11匹馬血漿LPS、ADPN、胰島素、血糖、IL-6、p38MAPK、5-HT、ET-1、TXB2和NO濃度均在不同時(shí)段變化顯著,對(duì)照組馬匹血漿濃度無顯著變化。LPS濃度從8 h時(shí)顯著上升,持續(xù)到40h,在16 h達(dá)到峰值,與0 h相比差異極顯著(P0.01)。內(nèi)分泌因子ADPN和胰島素分別從4 h、8 h顯著上升,在36 h分別達(dá)到最低值和峰值,與0 h相比差異極顯著(P0.01);血糖從4 h顯著升高并達(dá)到峰值,與0 h相比差異極顯著(P0.01),而后迅速降低,在16h恢復(fù)至正常水平。炎性指標(biāo)IL-6從24 h顯著升高,持續(xù)至68 h,在32 h達(dá)到峰值,與0h相比差異極顯著(P0.01)。p38MAPK從8 h顯著升高,在16 h達(dá)到峰值,與0 h相比差異極顯著(P0.01);血管收縮劑5-HT、ET-1、TXB2分別從4 h、8 h、8 h顯著上升,持續(xù)至60 h左右,分別在24 h、28 h、28 h達(dá)到峰值,與0 h相比差異極顯著(p0.01);NO濃度在8 h顯著上升,持續(xù)至60 h,在32 h達(dá)到峰值,與0 h相比差異極顯著(P0.01)。結(jié)果表明在OF蹄葉炎模型中,大量LPS入血導(dǎo)致IL-6、ET-1、ADPN和胰島素等多種物質(zhì)分泌紊亂,誘發(fā)蹄葉炎;LPS、ADPN、胰島素、IL-6、5-HT、ET-1、TXB2和NO的濃度變化趨與蹄葉炎病程變化相符。
[Abstract]:The disease of sport system is a kind of disease that does great harm to equine, and phlogistic is the most common disease in the disease of sport system. Based on this, we studied the lipopolysaccharide (LPS), adiponectin (ADPN), insulin, blood glucose in the blood circulation of equine fructooligosaccharide (OF) acute hoof phlogistic model. In order to explore the pathogenesis of hoofleaf disease, the concentration trend and pathological changes of interleukin-6 (IL-6) and p38 mitogen-activated protein kinase (p38 MAPKK, 5-HT, ET-1, TXB2, TXB2) and nitric oxide (no) were studied in order to explore the pathogenesis of hoofleaf disease. It can be used as a biomarker for early and rapid diagnosis and provides a theoretical basis for comprehensive prevention and treatment of hoofnut disease. Twenty-one Mongolian horses were randomly divided into experimental group and control group. The experimental group was given 10 g/kg weight of OF, while the control group was given distilled water. Blood samples were collected every 4 hours until 72 hours. The limping degree was evaluated by Obel grading standard. A horse with grade 3 lameness was euthanized. The third phalanx was cut off along the sagittal plane of the hoof, and the leaf layer of the hoof was stained by HE and PAS staining. The results showed that 11 horses in the experimental group showed clinical symptoms such as elevated body temperature, rapid breathing, rapid heart rate, lameness, diarrhea, etc. In the experimental group, 11 horses with clinical symptoms appeared first grade claudication from 8 to 20 hours after administration of OF and 24 to 56 hours of secondary claudication. With the appearance of secondary lameness, the respiration rate and heart rate of the horse increased, the pH value of feces decreased to the lowest value at 20 h, and the rectal temperature reached the peak at 40 h. All the horses in the experimental group developed diarrhea between 12 and 24 hours, and all the clinical symptoms gradually decreased after 60 hours. The horses in the control group had no symptoms such as elevated body temperature, rapid breathing, rapid heart rate, claudication, diarrhea and other clinical symptoms. X-ray examination showed that the third phalanx bone of the horses in the experimental group rotated. In the control group, the third phalanx of the control group was not rotated. He and PAS staining showed that there were inflammatory cells infiltrating in the leaf layer of the horse's hoof in the experimental group, the basophilic dye gathered around the blood vessel, cell deformation and other histological changes were found, but no histological changes were found in the leaf layer of the horse's hoof in the control group. In the experimental group, the plasma levels of LPS-ADPN, insulin, IL-6 / p38MAPKK _ (5-HTT _ (-1) TXB2 and no were significantly changed in 11 horses with clinical symptoms, while in the control group, there was no significant change in plasma concentration. The concentration of LPS increased significantly from 8 h to 8 h. After 40 h, the peak value was reached at 16 h, and the difference between 0 h and 0 h was significant (P 0.01). The endocrine factor ADPN and insulin increased significantly from 4 h to 8 h, and reached the lowest value and peak value at 36 h, respectively, and the difference was very significant compared with 0 h (P 0.01), and the blood glucose increased significantly from 4 h to the peak. Compared with 0 h, the difference was very significant (P 0.01), then decreased rapidly, and returned to normal level at 16 h. The inflammatory index IL-6 increased significantly from 24 h to 68 h, reached the peak at 32 h, and increased significantly from 8 h to 16 h compared with 0 h. Compared with 0 h, 5-HTET-1 TXB2 increased significantly from 4 h to 8 h, lasted for about 60 h, and reached its peak at 24 h, 28 h and 28 h, respectively. Compared with 0 h, the concentration of no in 5-HTET-1 + TXB2 increased significantly at 8 h. After 60 h, the peak value was reached at 32 h, and the difference between 0 h and 0 h was significant (P 0.01). The results showed that in the model of hoof leaf inflammation, a large amount of LPS induced the disturbance of secretion of IL-6ET-1 ADPN and insulin, and induced the changes of the concentrations of TXB2 and no in LPSN, IL-65-HTET-1, TXB2 and no were consistent with the course of philitis.
【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.21

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