本文選題：大豆肽 + 仔豬��； 參考：《江南大學(xué)》2016年碩士論文

【摘要】：大豆肽是由大豆分離蛋白經(jīng)復(fù)合酶水解后獲得的小肽,其低分子量(相對分子質(zhì)量主要為150-1500 Da)的特點(diǎn)使其具備很高的應(yīng)用價值。本文研究了大豆肽對仔豬血液生化指標(biāo)、腸道組織結(jié)構(gòu)和消化酶活性、免疫功能、氧化還原狀態(tài)、細(xì)胞因子相關(guān)基因及先天免疫受體基因表達(dá)量等的影響,初步探討大豆肽的功能及可能的作用機(jī)制,從而為更好地開發(fā)大豆肽作為新型安全的植物蛋白原料提供一定理論依據(jù)。首先以DPPH·和·OH的清除率為指標(biāo),對大豆肽在體外的抗氧化活性進(jìn)行測定。接著選取健康的7日齡“杜×長×大”仔豬12頭,分別標(biāo)記耳號,以雌雄各半的比例分為兩組。于每天早上9:00灌胃一次,實(shí)驗(yàn)組仔豬灌胃大豆肽溶液250 mg/kg·bw,對照組仔豬灌注丙氨酸溶液254 mg/kg·bw的。實(shí)驗(yàn)周期為14天。整個實(shí)驗(yàn)期間仔豬由母豬常規(guī)哺乳,同時統(tǒng)一飼喂乳豬前期配合飼料,記錄初重和末重,試驗(yàn)結(jié)束時屠宰取樣。根據(jù)灌胃實(shí)驗(yàn)結(jié)果,選取分別標(biāo)記耳號的、健康的25日齡“杜×長×大”斷奶仔豬l44頭,以12頭豬作為一個重復(fù),三個重復(fù)為一組,隨機(jī)分為4組。分別飼喂含0、5、10、20 g/kg大豆肽的日糧,飼喂周期28天。記錄仔豬始末重及飼料消耗量。實(shí)驗(yàn)結(jié)束時,每個處理組的12頭仔豬被隨機(jī)選取,經(jīng)前腔靜脈采集血樣進(jìn)行檢測。實(shí)驗(yàn)結(jié)果顯示:(1)大豆肽對DPPH·和·OH的清除率可分別高達(dá)98.33%、84.38%,結(jié)果顯示大豆肽的抗氧化能力較高,能發(fā)揮良好的清除自由基的功效。(2)與對照組相比,大豆肽可提高哺乳仔豬的日增重、血糖濃度,顯著降低血漿尿素氮濃度(P0.05);大豆肽顯著降低了小腸各段的隱窩深度及絨毛高度與隱窩深度的比值(V/C)(P0.05),小腸胰蛋白酶(P0.05)及二糖酶(P0.01)的活性均顯著升高;回腸派伊爾結(jié)(PP)的數(shù)量顯著增加,血漿IgG、IL-6和IFN-γ的濃度也顯著提高(P0.01);回腸黏膜sIgA、IL-6和IFN-γ的含量也顯著增加;血漿及組織(肝臟、脾臟、十二指腸、空腸、回腸)中SOD的活性得到極顯著的提高(P0.01),同時血漿的T-AOC能力也極顯著提升(P0.01);經(jīng)相關(guān)性分析顯示,血漿T-AOC和SOD活性與IgG的含量呈顯著正相關(guān)(P0.05);回腸黏膜和肝臟的IFN-γ、IL-6、IL-10、FOXP3和TGFβ1的基因表達(dá)均顯著上調(diào)(P0.05),回腸TLR2、TLR4、TLR9和NF-κB2基因表達(dá)量均得到顯著上調(diào)(P0.05)。(3)添加10 g/kg的大豆肽可以顯著提高斷奶仔豬的日增重、日采食量(P0.05),料重比也顯著降低(P0.05);同時還可以顯著提高斷奶仔豬的血漿IgG、IgM、IL-6以及IFN-γ含量(P0.05)。綜上所述,大豆肽能促進(jìn)仔豬腸黏膜組織結(jié)構(gòu)發(fā)育,提高消化酶活性,促進(jìn)仔豬對營養(yǎng)的消化吸收,提高腸黏膜免疫功能,改善仔豬生長性能。同時大豆肽有助于提高仔豬抗氧化能力,上調(diào)免疫相關(guān)基因表達(dá)量,從而提高IgG和sIgA含量,增強(qiáng)仔豬免疫功能,更好促進(jìn)仔豬的健康。
[Abstract]:Soy peptide is a small peptide obtained by hydrolysis of soybean protein isolate after complex enzyme hydrolysis. Its low molecular weight (relative molecular weight is mainly 150-1500 Da) has high application value. In this paper, the blood biochemical index, intestinal structure and digestive enzyme activity, immune function, redox state and cell cause of soybean peptide to piglet were studied. The effects of subrelated genes and the expression of innate immune receptor gene are discussed, and the function and possible mechanism of soybean peptides are preliminarily discussed in order to provide a certain theoretical basis for the better development of soybean peptide as a new safe plant protein raw material. First, the antioxidant activity of soybean peptide in vitro is based on the scavenging rate of DPPH and. OH. Then, 12 healthy 7 days old "Du x long x" piglets were selected to mark the ears, respectively, to be divided into two groups in the proportion of male and male. At 9:00 every morning, the experimental group was intragastric at 9:00. The experimental group was filled with soybean peptide solution of 250 mg/kg. Bw. The control group was perfused with alanine solution of 254 mg/kg. BW. The experiment period was 14 days. The experiment period was the whole experiment period. According to the results of the experiment, the L44 head of a healthy 25 day old Weanling Piglet, "Du x long x big", was selected as a repeat and three repetition as a group and divided into 4 randomly divided into 4 according to the results of the gavage experiment. Group. Fed with 0,5,10,20 g/kg soybean peptide diet, feeding cycle for 28 days. Record the weight and feed consumption of piglets. At the end of the experiment, 12 piglets in each treatment group were randomly selected and collected through the anterior vena cava. The results showed: (1) the scavenging rate of soybean peptide to DPPH and OH could be as high as 98.33%, 84.38%, respectively. The results showed that the antioxidant capacity of soybean peptide was higher and the free radical scavenging effect was good. (2) compared with the control group, soybean peptide could increase the daily weight gain, blood glucose concentration, and significantly lower the plasma urea nitrogen concentration (P0.05), and the soybean peptide significantly reduced the depth of the recess in the small intestine and the ratio of the height of the villi to the depth of the recess. V/C) (P0.05), the activity of trypsin (P0.05) and two glucoenzyme (P0.01) in the small intestine increased significantly, the number of PP in ileum increased significantly, and the concentration of IgG, IL-6 and IFN- y in plasma was also significantly increased (P0.01); the contents of sIgA, IL-6 and IFN- gamma in the ileum mucosa were also significantly increased; plasma and tissues (liver, spleen, duodenum, jejunum, and ileum) were also increased. The activity of the plasma was significantly improved (P0.01), and the T-AOC ability of plasma was significantly increased (P0.01). The correlation analysis showed that the activity of plasma T-AOC and SOD was positively correlated with the content of IgG (P0.05), and the gene expression of IFN- gamma, IL-6, IL-10, FOXP3 and TGF beta 1 in the ileum mucosa and liver were significantly up-regulated. The expression of NF- kappa B2 gene expression was significantly up-regulated (P0.05). (3) adding 10 g/kg soybean peptide could significantly increase the daily weight gain of weaned piglets, daily feed intake (P0.05), and significantly lower feed weight ratio (P0.05). At the same time, the plasma IgG, IgM, IL-6 and IFN- gamma content of weaned piglets were significantly increased (P0.05). To sum up, soybean peptides could promote piglets. The tissue structure of the intestinal mucosa is developed, the digestive enzyme activity is raised, the piglets can improve the digestion and absorption of nutrition, improve the immune function of the intestinal mucosa and improve the growth performance of the piglets. At the same time, the soybean peptide can improve the antioxidant capacity of piglets and raise the expression of the immune related genes, thus improve the content of IgG and sIgA, enhance the immune function of piglets, and promote the piglets better. Health.
【學(xué)位授予單位】：江南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S828.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙芳芳 ,張日俊;大豆肽研究進(jìn)展[J];中國飼料;2004年01期

2 任善茂,陶勇;飼用大豆肽的開發(fā)應(yīng)用前景[J];飼料研究;2004年10期

3 武軍,閆海洋;固定化酶制備大豆肽的研究[J];農(nóng)產(chǎn)品加工(學(xué)刊);2005年Z2期

4 鄒玉蓉;;大豆肽的特性及在飼料中的應(yīng)用研究進(jìn)展[J];飼料研究;2007年03期

5 劉偉;孫鶴;馬德松;王新;;富硒大豆肽健康飲料的研制[J];大豆科技;2010年06期

6 劉小飛;;大豆肽對日糧營養(yǎng)素轉(zhuǎn)化的影響及其在動物生產(chǎn)的應(yīng)用[J];飼料廣角;2012年07期

7 李小東,趙冬梅;大豆肽奶的研制及加工技術(shù)研究[J];大豆通報;2004年05期

8 張偉;王艷萍;;大豆肽的特性及其營養(yǎng)保健功能[J];農(nóng)技服務(wù);2008年11期

9 陳濟(jì)琛;李善仁;蔡海松;林新堅(jiān);;大豆肽的制備及其在養(yǎng)殖業(yè)中的應(yīng)用[J];大豆科學(xué);2009年02期

10 廖斌;李建科;王慧玲;李鑫;楊有仙;;大豆肽的特性及制備方法研究[J];糧食與飼料工業(yè);2012年01期

相關(guān)會議論文 前2條

1 吳海波;江連洲;陳巖;;降脂大豆肽的制備及其功能測定[A];第23屆全國大豆科研生產(chǎn)研討會論文摘要集[C];2012年

2 張延坤;郭大發(fā);石勝堯;劉國忠;朱素英;房玉水;;大豆肽的制備與應(yīng)用研究[A];中國營養(yǎng)學(xué)會第五次營養(yǎng)資源與保健食品學(xué)術(shù)會議論文摘要匯編[C];1999年

相關(guān)重要報紙文章 前3條

1 文言;我國自主研發(fā)提能大豆肽面市[N];中國貿(mào)易報;2006年

2 韓娉婷　王世冰;國內(nèi)首家大豆肽、多糖項(xiàng)目落戶清豐[N];農(nóng)民日報;2011年

3 王穎;大豆肽為開發(fā)老年食品帶來新契機(jī)[N];中國食品質(zhì)量報;2004年

相關(guān)碩士學(xué)位論文 前10條

1 孫莉潔;大豆肽的分離純化及應(yīng)用研究[D];東北林業(yè)大學(xué);2009年

2 趙芳芳;大豆肽的生物學(xué)功能研究[D];中國農(nóng)業(yè)大學(xué);2004年

3 黃文凱;膜分離方法制備免疫活性大豆肽的研究[D];江南大學(xué);2007年

4 薛靜玉;酶法生產(chǎn)大豆肽及其特性的研究[D];河南工業(yè)大學(xué);2012年

5 高綠莎;大豆肽緩解體力疲勞活性研究與產(chǎn)品研制[D];吉林大學(xué);2015年

6 段娜娜;大豆肽的開發(fā)及產(chǎn)業(yè)化可行性研究報告[D];河南工業(yè)大學(xué);2011年

7 李紅勝;大豆肽的制備及其對小鼠免疫功能的影響[D];廣東海洋大學(xué);2012年

8 左倩;大豆肽對仔豬免疫和消化功能的影響[D];江南大學(xué);2016年

9 姜曼;固態(tài)發(fā)酵豆粕制備大豆肽發(fā)酵條件的優(yōu)化[D];山東輕工業(yè)學(xué)院;2010年

10 褚斌杰;大豆肽的分離及其生理功能鑒定[D];華中農(nóng)業(yè)大學(xué);2011年

，

本文編號：1913191