本文選題：奶牛 + miRNA-��； 參考：《中國獸醫(yī)學報》2017年01期

【摘要】：利用生物信息學方法預測出miR-224可能的靶基因為ACADM、ACAT1、ALDH2和LPL,然后用實時熒光定量法檢測了miR-224與其靶基因在奶牛乳腺上皮細胞中的表達量。結(jié)果顯示:(1)miR-224的相對表達量。轉(zhuǎn)染shNc組表達量低于轉(zhuǎn)染mimics組而高于inhibitor組。(2)靶基因熒光定量結(jié)果表明,ACADM、ALDH2和LPL基因轉(zhuǎn)染shNc組表達量低于轉(zhuǎn)染inhibitor組而高于mimics組。(3)經(jīng)SPSS22.0相關性分析表明:在乳腺上皮細胞中,ACADM、ALDH2和LPL基因的表達量與miR-224的表達量呈顯著負相關,ACAT1基因與miR-224沒有顯著聯(lián)系。初步驗證說明,ACADM、ALDH2和LPL基因可能是miR-224的靶基因,為進一步探索miR-224在奶牛乳牛乳腺上皮細胞發(fā)揮的作用提供了一定的理論依據(jù)和研究基礎。
[Abstract]:The possible target of miR-224 was predicted by bioinformatics method. Then the expression of miR-224 and its target gene in bovine mammary epithelial cells was detected by real-time fluorescence quantitative method. The results showed the relative expression of miR-224. The results of fluorescence quantitative analysis of target gene in shNc group were lower than those in mimics group but higher than that in inhibitor group. The results showed that the expression of target gene in shNc group was lower than that in inhibitor group but higher than that in mimics group.) the results of SPSS22.0 correlation analysis showed that: in fine mammary epithelium, the expression of ACA DMN ALDH2 and LPL gene in shNc group was higher than that in inhibitor group. There was significant negative correlation between the expression of ALDH2 and LPL genes and the expression of miR-224. There was no significant correlation between ACAT1 gene and miR-224. The preliminary verification indicated that ALDH2 and LPL genes may be the target genes of miR-224, which provides a theoretical basis and research basis for further exploring the role of miR-224 in dairy cow mammary epithelial cells.
【作者單位】： 黑龍江八一農(nóng)墾大學動物科學學院;吉林大學動物科技學院;
【基金】：國家自然科學基金資助項目(31200922) 黑龍江省自然科學基金資助項目(QC2012C054) 黑龍江省教育廳項目(12521365)
【分類號】：S823

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