本文選題：雞傳染性腺胃炎 + 流行病學(xué)��； 參考：《聊城大學(xué)》2015年碩士論文

【摘要】：雞傳染性腺胃炎是一種由多種原因引起的以腺胃腫大、腺胃乳頭出血潰瘍?yōu)橹匾卣鞯穆韵佬缘膫魅静?是危害養(yǎng)禽業(yè)的重要疾病之一。本試驗(yàn)先對(duì)其流行病學(xué)進(jìn)行調(diào)查研究,后從機(jī)體免疫學(xué)角度入手,研究雞傳染性腺胃炎發(fā)生過程中7種不同細(xì)胞因子的變化情況;采用MTT法對(duì)21日齡的發(fā)病雞進(jìn)行外周血淋巴細(xì)胞的分離、培養(yǎng)和增殖反應(yīng)活性檢測(cè),研究感染雞傳染性腺胃炎后機(jī)體內(nèi)新城疫和禽流感抗體的變化情況;觀察該病的組織病理學(xué)變化,了解雞傳染性腺胃炎的發(fā)病機(jī)制,為雞傳染性腺胃炎的有效防控奠定基礎(chǔ)。1.雞傳染性腺胃炎的流行病學(xué)調(diào)查雞傳染性腺胃炎是危害養(yǎng)禽業(yè)的主要疾病之一,主要癥狀為采食量下降,生長(zhǎng)緩慢,腺胃腫大,腺胃乳頭粘膜脫落、出血甚至潰瘍等。該病主要侵害3-25d的雞群,病死率高達(dá)30%,病程較長(zhǎng),可持續(xù)10-15d。12個(gè)不同地區(qū)同一時(shí)間的雞群均發(fā)生雞傳染性腺胃炎,表明該病在不排除疫苗污染的情況下傳播方式可能是垂直傳播。2.感染雞傳染性腺胃炎后機(jī)體內(nèi)細(xì)胞因子含量的變化采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)對(duì)發(fā)病組和健康組的雞群采樣進(jìn)行細(xì)胞因子的測(cè)定,發(fā)現(xiàn)感染雞傳染性腺胃炎后的雞群體內(nèi)IL-1、IL-6、IL-8、TNF-ɑ、SIg A等細(xì)胞因子的含量高于健康雞群,而IL-2和IFN-γ的含量低于健康雞群。在病變最典型的21d,感染雞傳染性腺胃炎的雞群體內(nèi)細(xì)胞因子的含量變化最為顯著,結(jié)果表明雞群感染傳染性腺胃炎后出現(xiàn)明顯的炎癥反應(yīng),導(dǎo)致與免疫相關(guān)的細(xì)胞因子發(fā)生相應(yīng)的變化。3.感染雞傳染性腺胃炎對(duì)機(jī)體免疫應(yīng)答的影響通過MTT法測(cè)定21d雞外周血淋巴細(xì)胞的增殖反應(yīng),發(fā)現(xiàn)發(fā)病雞機(jī)體內(nèi)T淋巴細(xì)胞濃度高于健康對(duì)照組,但差異性不顯著。通過HA和HI法對(duì)雞新城疫和禽流感抗體水平進(jìn)行測(cè)定,發(fā)現(xiàn)健康雞的抗體水平在14d時(shí),禽流感H9抗體水平明顯高于發(fā)病雞,其余時(shí)間差異不顯著,而新城疫抗體水平差異性不顯著,所以雞傳染性腺胃炎的發(fā)生對(duì)機(jī)體的疫苗免疫反應(yīng)有選擇性的部分抑制作用。4.雞傳染性腺胃炎的病理組織學(xué)觀察制備相關(guān)器官(如腺胃、心臟、肝臟、脾臟、腎臟等)的病理組織切片,通過HE染色,觀察其病理組織學(xué)變化。結(jié)果表明,患病雞的腺胃、心臟、肝臟、脾臟等器官有明顯的出血,并伴有大量的炎性細(xì)胞浸潤(rùn),腺胃固有層、黏膜下層等不同部位都出現(xiàn)水腫,同時(shí)出現(xiàn)腺胃乳頭出血,腺小管間淤血等現(xiàn)象。這些病理組織學(xué)變化與臨床出現(xiàn)腺胃明顯腫大、腺胃乳頭出血、潰瘍等剖檢特征相一致。
[Abstract]:Infectious glandular gastritis is a chronic digestive tract infectious disease which is caused by a variety of causes, such as adeno and gastric enlargement and gastric papillary bleeding and ulcer, which is one of the most important diseases that harm the poultry industry. This experiment first investigated the epidemiology of the disease, and then began to study the occurrence of infectious glandular gastritis from the point of immunity from the organism. The change of 7 different cytokines in the process, the MTT method was used to separate the peripheral blood lymphocytes of the 21 day old chickens, to detect the activity of the culture and proliferation reaction, to study the changes of the Newcastle disease and the avian influenza antibody in the infected chicken infectious gland gastritis, and to observe the histopathological changes of the disease and to understand the infectious gonadal gland of the chicken. The pathogenesis of gastritis, laying the foundation for the effective prevention and control of infectious glandular gastritis, the epidemiological investigation of infectious adenogastritis in chicken.1., chicken infectious adenogastritis is one of the main diseases endangering the poultry industry. The main symptoms are the decline in intake, slow growth, glandular stomach swelling, glandular mucous mucous membrane exfoliation, bleeding even ulcers. The fatality rate is up to 30% and the course of disease is long. The chicken infective gastritis of chickens in different regions of the same time in different regions of the 10-15d.12 can be infected by 3-25d, which indicates that the transmission mode of the disease may be the change of the cytokine content in the body of infectious glandular gastritis in the vertical transmission of.2. infection without excluding the contamination of the vaccine. The immuno adsorption test (ELISA) was used to determine the cytokines in the chickens of the disease group and the healthy group. It was found that the contents of IL-1, IL-6, IL-8, TNF-, and SIg A in the chickens infected with infectious chicken gastritis were higher than those of healthy chickens, but the content of IL-2 and IFN- gamma was lower than that of the healthy chickens. The infection of chicken in the most typical 21d of the disease was infected with chickens. The changes in the content of cytokines in the chicken group of gonadal gastritis were the most significant. The results showed that the chickens infected with infectious gonadal gastritis had obvious inflammatory response, which resulted in the corresponding changes in the immune related cytokines. The effect of.3. infection on the immune response of chicken infectious glandular gastritis was determined by MTT method for the determination of the peripheral blood lymph of 21d chickens. The proliferation reaction of the cell was found to be higher than that in the healthy control group, but the difference was not significant. The antibody level of Newcastle disease and avian influenza was measured by HA and HI, and the antibody level of the healthy chicken was higher than that of the disease chicken when the antibody level of the healthy chicken was at 14d. The difference of the rest time was not significant, but the new Newcastle disease was not significant. The difference of antibody level is not significant, so the occurrence of avian infectious adenogastritis has a selective partial inhibitory effect on the immune response of the body. The histopathological sections of the related organs (such as the stomach, heart, liver, spleen, kidney, etc.) of.4. infectious glandular gastritis (such as adeno stomach, heart, liver, spleen, kidney and so on) are prepared, and the pathological histology is observed by HE staining. The results showed that the glandular stomach, heart, liver, spleen and other organs of the sick chicken had obvious bleeding, accompanied by a large number of inflammatory cell infiltration, edema in the propria of the glands and the submucosa and other parts of the submucosa, and the phenomena of glandular gastric papilla bleeding and glandular intertubule congestion. The features of large, bleeding of gastric glands, bleeding and ulcer were consistent.
【學(xué)位授予單位】：聊城大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.31

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 沙永剛,程國(guó)富,邵華斌;雞分泌型免疫球蛋白A相關(guān)研究進(jìn)展[J];養(yǎng)禽與禽病防治;2003年08期

2 李杏媛;王海富;張惠清;;雞白細(xì)胞介素-2分子生物學(xué)和疾病防治應(yīng)用研究進(jìn)展[J];中國(guó)家禽;2010年13期

3 楊倩,黃國(guó)慶,毛衛(wèi)華,王紅麗;不同免疫途徑對(duì)蛋用雞分泌型免疫球蛋白A細(xì)胞分布的影響[J];中國(guó)獸醫(yī)科技;1998年11期

4 褚艷麗;張秀美;胡北俠;黃艷艷;郭立輝;劉思當(dāng);;雞傳染性腺胃病病原的分離與鑒定[J];中國(guó)獸醫(yī)雜志;2008年02期

5 陳玉華;母安雄;王曉陽;;3種雞免疫抑制性疫病對(duì)禽流感疫苗免疫效果的影響[J];浙江農(nóng)業(yè)科學(xué);2014年01期

6 李衛(wèi)華;;肉雞傳染性腺胃炎與傳染性法氏囊病混合感染的診治[J];中國(guó)畜牧獸醫(yī)文摘;2013年05期

，

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