本文選題：C型產(chǎn)氣莢膜梭菌類毒素疫苗 + 懷孕母豬��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：為制備具有高效保護力的C型產(chǎn)氣莢膜梭菌類毒素疫苗,利用C型產(chǎn)氣莢膜梭菌標準菌株(NCTC 3180)所產(chǎn)外毒素經(jīng)甲醛滅活并加入佐劑乳化后,制備了C型產(chǎn)氣莢膜梭菌類毒素疫苗;將該疫苗免疫母豬后檢測母豬初乳和仔豬血清的抗體效價;用制備的C型產(chǎn)氣莢膜梭菌類毒素疫苗分別多次免疫豬,隔段時間后采集血液,制備血清,測定血清中的抗毒素效價。本研究分為3部分進行:第一部分:C型產(chǎn)氣莢膜梭菌類毒素疫苗的制備。對C型產(chǎn)氣莢膜梭菌標準菌種(NCTC3180)進行復(fù)蘇、增菌、產(chǎn)毒,再用0.22μm濾器過濾除去菌體得到毒素溶液,然后用甲醛對毒素進行滅活后,分別加入白油佐劑和氫氧化鋁膠佐劑制得兩種C型產(chǎn)氣莢膜梭菌類毒素疫苗。第二部分:類毒素疫苗的檢驗。參照《中華人民共和國獸用生物制品質(zhì)量標準》分別對疫苗進行了安全性檢驗,無菌檢驗,免疫效力檢驗。分別在分娩前30和15天免疫接種懷孕母豬,通過間接ELISA和毒素中和試驗評估母豬初乳和14日齡仔豬血液中抗體效價水平。結(jié)果顯示,母豬初乳和仔豬血清中抗毒素效價分別為1:3200,1:1600,母豬初乳和仔豬血液中的中和效價均為1:6。表明制備的類毒素疫苗效價高并安全可靠,免疫實驗動物后能對仔豬起到一定的保護作用。第三部分:抗毒素血清的制備。采用制備的C型產(chǎn)氣莢膜梭菌白油佐劑類毒素疫苗多次免疫豬,采集血液,制備血清,參照《中華人民共和國獸用生物制品質(zhì)量標準》分別對血清進行了安全性檢驗和無菌檢驗;通過間接ELISA法和小鼠的血清中和試驗測定血清效價。結(jié)果顯示:血清的安全性檢驗和無菌檢驗均符合標準;間接ELISA法測定豬血清中的抗α抗體效價為1:6400;間接ELISA法測定豬血清中的抗β抗體效價為1:12800。0.1m L的C型產(chǎn)氣莢膜梭菌高免血清可以中和500個MLD。
[Abstract]:In order to prepare Clostridium perfringens type C toxoid vaccine with high protective power, the toxin produced by Clostridium perfringens strain NCTC 3180 was inactivated by formaldehyde and emulsified with adjuvant, then the toxin vaccine of Clostridium perfringens type C was prepared. After immunizing sows, the antibody titers of sows colostrum and piglets serum were detected, the pigs were immunized with the C type Clostridium perfringens toxoid vaccine for several times, and the blood samples were collected at intervals to prepare serum. The antitoxin titers in serum were determined. This study is divided into three parts: the first part is the preparation of Clostridium perfringens toxoid vaccine. The standard strain of Clostridium perfringens type C, NCTC3180), was resuscitated, increased bacteria, produced toxin, filtered the bacteria with 0.22 渭 m filter to get toxin solution, then inactivated the toxin with formaldehyde. Two C-type Clostridium perfringens toxoid vaccines were prepared by adding white oil adjuvant and aluminum hydroxide adjuvant respectively. The second part: the examination of toxoid vaccine. With reference to the quality Standard of Veterinary Biological products of the people's Republic of China, the safety, aseptic and immunological efficacy of the vaccine were tested. Pregnant sows were immunized 30 and 15 days before delivery. The antibody titers in colostrum and 14 day piglets were evaluated by indirect ELISA and toxin neutralization tests. The results showed that the antitoxin titers in serum of sows and piglets were 1: 3 200 and 1: 1600, respectively, and the neutralization titers in colostrum and blood of sows were 1: 6, respectively. The results showed that the prepared toxoid vaccine had high titer, safety and reliability, and could protect piglets after immunizing experimental animals. Part three: preparation of antitoxin serum. Pigs were immunized with C type Clostridium perfringens white oil adjuvant toxoid vaccine for many times. Blood was collected and serum was prepared. According to the quality standard of animal biological products of the people's Republic of China, the safety test and aseptic test of serum were carried out, and the titer of serum was determined by indirect ELISA method and serum neutralization test of mice. The results showed that the safety test and aseptic test of serum were in accordance with the standard. The titer of anti 偽 antibody in pig serum was 1: 6400 by indirect ELISA method, and 500 MLDs could be neutralized by indirect ELISA assay when the titer of anti 尾 antibody in swine serum was 1: 12800. 1m L and that of Clostridium perfringens type C high immune serum was 1: 6400.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.4

【參考文獻】

相關(guān)期刊論文 前2條

1 張小榮,文其乙,劉秀梵,焦新安;致人腹瀉產(chǎn)氣莢膜梭菌的分離鑒定與基因分型[J];中國人獸共患病雜志;2002年04期

2 陳小云,萬建青,程君生,關(guān)孚時,張存帥;從A型產(chǎn)氣莢膜梭菌C57-1中發(fā)現(xiàn)β2毒素基因[J];中國獸藥雜志;2005年05期

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