本文選題：環(huán)形泰勒蟲 + 流行病學(xué)調(diào)查　； 參考：《新疆農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：牛環(huán)形泰勒蟲病是由攜帶該病原的蜱叮咬和感染牛所致的一種原蟲病。該病以高熱、消瘦、貧血和周身淋巴結(jié)腫脹為特征,常引起牛致死性白細(xì)胞增殖紊亂。國(guó)際獸醫(yī)局(OIE)把該病定為必須通報(bào)的重大動(dòng)物疾病。牛環(huán)形泰勒蟲的傳播媒介為璃眼蜱屬,在新疆分布較廣,所攜帶的病原感染牛導(dǎo)致泰氏焦蟲病常見、多發(fā),嚴(yán)重危害養(yǎng)牛業(yè)的可持續(xù)發(fā)展,是重點(diǎn)防控的寄生蟲病。因此,為了更好地對(duì)吐魯番托克遜縣部分地區(qū)牛環(huán)形泰勒蟲病實(shí)施防控,本研究調(diào)查了解牛環(huán)形泰勒蟲病及其傳播媒介蜱的地方性流行規(guī)律,闡明環(huán)形泰勒蟲病在當(dāng)?shù)氐牧餍鞋F(xiàn)狀。本文對(duì)隨機(jī)采自托克遜縣夏鄉(xiāng)南湖村的蜱蟲和牛全血進(jìn)行試驗(yàn),通過顯微鏡蜱種鑒定發(fā)現(xiàn)該地牛環(huán)形泰勒蟲的傳播媒介蜱為小亞璃眼蜱。通過血液涂片檢查,共104份血液樣品中有53份發(fā)現(xiàn)了圓點(diǎn)狀或戒指狀的蟲體,根據(jù)環(huán)形泰勒蟲的病原形態(tài)可確診這些蟲體為牛環(huán)形泰勒蟲,即陽(yáng)性率為51.0%。統(tǒng)計(jì)不同年齡段牛只環(huán)形泰勒蟲血涂片的感染情況,結(jié)果顯示0~6月齡的牛對(duì)環(huán)形泰勒蟲的感染率最高,為71.4%,與吐魯番地區(qū)相關(guān)報(bào)道結(jié)果(1~3歲)不一致;分別計(jì)算各年齡段牛紅細(xì)胞染蟲率,發(fā)現(xiàn)帶蟲率不超過5%。統(tǒng)計(jì)18家養(yǎng)牛戶血涂片的感染情況,發(fā)現(xiàn)有2家養(yǎng)牛戶感染率為100%,有3家養(yǎng)牛戶感染率為0,有10家養(yǎng)牛戶的感染率不小于50%。本試驗(yàn)基本查清了目前牛環(huán)形泰勒蟲在托克遜縣夏鄉(xiāng)南湖村的流行情況,可為當(dāng)?shù)嘏－h(huán)形泰勒蟲病的綜合防治提供參考資料。本試驗(yàn)參考Christine D’Oliveira中已發(fā)表的牛環(huán)形泰勒蟲Tams1基因序列,合成一對(duì)特異性引物,提取環(huán)形泰勒蟲病原DNA,對(duì)吐魯番托克遜縣隨機(jī)104頭份牛的全血進(jìn)行了PCR檢測(cè),并在特異性和靈敏度方面與血液涂片方法進(jìn)行對(duì)比檢測(cè)。結(jié)果表明,PCR方法檢出率(57.7%)高于血涂片的檢出率(51.0%)。對(duì)不同年齡段牛的感染情況,PCR結(jié)果顯示1~2歲的牛對(duì)環(huán)形泰勒蟲的感染率最高,為72.7%,與吐魯番地區(qū)相關(guān)報(bào)道結(jié)果(1~3歲)基本一致。各養(yǎng)牛戶PCR方法檢測(cè)感染率(56.1%)高于涂片染色鏡檢的感染率(48.1%),說明PCR檢測(cè)方法較血涂片方法特異性強(qiáng),靈敏度高。PCR診斷方法的建立為托克遜縣夏鄉(xiāng)南湖村牛泰式焦蟲病的早期診斷提供了技術(shù)支撐,試驗(yàn)參數(shù)更準(zhǔn)確地顯示了該地牛環(huán)形泰勒蟲的感染現(xiàn)狀,為我區(qū)的牛環(huán)形泰勒蟲病提供了防治依據(jù)。
[Abstract]:Bovine annular Taylor's disease is a protozoa caused by the bite and infection of ticks carrying the pathogen. The disease is characterized by high fever, emaciation, anemia and swelling of lymph nodes around the body, often leading to fatal bovine leukocyte proliferation disorder. The International Veterinary Bureau (OIEs) identifies the disease as a major animal disease that must be notified. The transmission medium of Taylor's annulus in cattle is Acari, which is widely distributed in Xinjiang, and the pathogen it carries is common and frequent in cattle, which seriously endangers the sustainable development of cattle industry and is a parasitic disease which is mainly controlled and prevented. Therefore, in order to better prevent and control bovine ringed Taylor disease in some areas of Turpan Tok Keson County, this study investigated the endemic laws of bovine ringed Taylor disease and its transmission vector ticks. To clarify the local prevalence of the circular Taylor's disease. In this paper, the whole blood of ticks and cattle collected from Nanhu Village, Xia Township, Tokeson County were randomly collected. It was found by microscopic identification that the transmission vector of Trichoderma annularis was Acari sibiricus. Through blood smear examination, 53 of 104 blood samples were found to have polka or ring shaped worms. According to the pathogenic morphology of the circular Taylor worm, the positive rate was 51. 0%, and the positive rate was 51. 0%. The results showed that the infection rate of 0- and 6-month-old cattle was the highest (71.4%), which was not consistent with the results reported in Turpan area. The rate of red blood cell infection of cattle in each age group was calculated, and the rate of carrying the worm was less than 5%. The infection rate of blood smears in 18 cattle households was analyzed. It was found that the infection rate was 100 in 2 cattle households, 0 in 3 cattle households, and no less than 50 in 10 cattle households. In this experiment, the current epidemic situation of the circular Taylor worm in the village of Nanhu, Xia Township, Tokson County was basically ascertained, which can provide reference for the comprehensive control of the disease of the cattle ring Taylor's disease in the local cattle. A pair of specific primers were synthesized with reference to the Tams1 gene sequence published in Christine D'Oliveira, and the whole blood samples of 104 cattle were detected by PCR. The specificity and sensitivity were compared with the blood smear method. The results showed that the detection rate of PCR was 57.7% higher than that of blood smear (51.0%). The results of PCR showed that the infection rate of 1 or 2 year old cattle was the highest (72.7%), which was consistent with the results reported in Turpan area (1 ~ 3 years old). The infection rate of PCR was 56.1% higher than that of smear stain microscopy (48.1%), indicating that the specificity of PCR was higher than that of blood smear. The establishment of the highly sensitive. PCR diagnostic method provided technical support for the early diagnosis of Taijiao disease of cattle in Xiaxiang Nanhu village of Tokson County, and the experimental parameters more accurately showed the current infection status of T. annulosa. It provides the basis for the prevention and control of bovine ringed Taylor's disease in our area.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.23

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