本文選題：脫氧雪腐鐮刀菌烯醇 + 雛雞�。� 參考：《西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年02期

【摘要】：【目的】通過DON暴露體內(nèi)試驗(yàn),根據(jù)雛雞腦組織中神經(jīng)遞質(zhì)含量及鈣調(diào)蛋白(CAM)含量的變化,研究DON對(duì)雛雞的神經(jīng)毒性作用。【方法】選取120只健康1日齡海蘭蛋雞(公)預(yù)飼1周后,按單因素試驗(yàn)設(shè)計(jì)隨機(jī)均分為DON低、中、高劑量組和對(duì)照組,對(duì)照組和試驗(yàn)組飼喂相同的全價(jià)日糧。DON低劑量組、中劑量組和高劑量組分別按采食量中含DON 0.27,1.68和12.21mg/kg的劑量灌胃,對(duì)照組灌服等量的生理鹽水。試驗(yàn)從第8天開始,每隔7d染毒1次,共染毒5次。試驗(yàn)期為36d。試驗(yàn)結(jié)束時(shí),每組隨機(jī)屠宰20只雞,迅速取出腦組織,檢測(cè)神經(jīng)遞質(zhì)含量和CAM含量的變化�！窘Y(jié)果】經(jīng)高效液相色譜-熒光檢測(cè)法(HPLC-FD)檢測(cè),雛雞腦組織中未發(fā)現(xiàn)5-羥色胺吲哚乙酸(5-HIAA);與對(duì)照組相比,DON高劑量組雛雞腦組織中去甲腎上腺素(NE)和5-羥色胺(5-HT)含量顯著升高(P0.05),而多巴胺(DA)含量顯著降低(P0.05)。與對(duì)照組相比,DON中、高劑量組雛雞神經(jīng)細(xì)胞內(nèi)游離鈣([Ca2+]i)濃度和腦組織中CAM含量顯著降低(P0.05),不同DON劑量組CAM mRNA基因相對(duì)表達(dá)量均顯著降低(P0.05)�！窘Y(jié)論】DON暴露可引起雛雞神經(jīng)鈣穩(wěn)態(tài)變化,并影響部分神經(jīng)遞質(zhì)的分泌,對(duì)雛雞具有一定的神經(jīng)毒性作用。
[Abstract]:[objective] to study the changes of neurotransmitters and calmodulin (calmodulin) contents in brain tissue of chicks by DON exposure in vivo. The neurotoxic effects of DON on chicks were studied. [methods] 120 healthy 1-day-old Hailan laying hens (males) were prefed for 1 week, and were randomly divided into low, middle, high dose DON groups and control groups according to the single factor design. The control group and the experimental group were fed with the same low-dose diet. The middle dose group and the high dose group were fed with the same dose of DON 0.27N 1.68 and 12.21mg/kg, respectively. The control group was given the same amount of normal saline. The experiment began on the 8th day and was administered once every 7 days for a total of 5 times. The experimental period is 36 days. At the end of the experiment, 20 chickens were slaughtered at random in each group, the brain tissue was removed quickly, and the changes of neurotransmitter and CAM content were detected. [results] HPLC-FDD was used to detect the content of neurotransmitter and CAM. Compared with the control group, the contents of NE) and 5-HT) in brain tissues of chicks with high dose of don were significantly higher than those of the control group, while the contents of dopamine (DA) decreased significantly in the brain tissues of chicks. In don compared with the control group, The concentration of free calcium ([Ca2] I) in nerve cells and the content of CAM in brain tissue decreased significantly in high dose group, and the relative expression of CAM mRNA gene decreased significantly in different DON dosage groups. [conclusion] DON exposure can induce steady state change of nerve calcium in chicks. It also affected the secretion of some neurotransmitters and had a certain neurotoxic effect on chicks.
【作者單位】： 安徽農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31472250) 安徽省教育廳重點(diǎn)項(xiàng)目(KJ2013A119)
【分類號(hào)】：S858.31

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