本文選題：湖羊 + 多乳頭��； 參考：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年01期

【摘要】：為了查明多乳頭湖羊(Ovis aries)(實(shí)驗(yàn)組)乳腺組織結(jié)構(gòu)和胰島素樣生長因子Ⅰ受體(insulin-like growth factor-I receptor,IGF-IR)水平是否與兩乳頭湖羊(對照組)有差異、是否有可能具有更高的泌乳性能,本研究分別采集了兩組湖羊育成期、妊娠期、泌乳期和空懷期的乳腺組織樣品,HE染色(hematoxylineosin staining)后觀察乳腺組織的形態(tài)學(xué)變化;q RT-PCR檢測乳腺組織的IGF-IR在不同生理階段的m RNA表達(dá)水平;免疫組織化學(xué)法(immunohistochemistry,IHC)檢測激素受體蛋白在乳腺組織的分布情況;Western blot法檢測激素受體蛋白表達(dá)水平。結(jié)果表明,育成期,兩組湖羊乳腺間質(zhì)增長較快,腺體中含有大量的結(jié)締組織和脂肪組織,且實(shí)驗(yàn)組湖羊乳腺導(dǎo)管數(shù)量比對照組羊較密集;妊娠期,大量的小葉腺泡發(fā)育完全,腺泡腔中充滿分泌物,腺泡數(shù)量多于對照組;泌乳期,兩組湖羊乳腺腺泡體積增大,腺泡大小、形狀不一,腺泡腔中含有豐富的乳汁;斷奶后,兩組湖羊乳腺腺泡逐漸塌陷,形態(tài)結(jié)構(gòu)發(fā)生變化。從育成期到泌乳期,兩組乳腺組織中的IGF-IR m RNA表達(dá)量和蛋白分布量逐漸增加,而對照組乳腺組織中的IGF-IR m RNA表達(dá)量從育成期到妊娠期逐漸增加,泌乳期較妊娠期稍有下調(diào);斷奶后,m RNA表達(dá)量和蛋白分布量均低于泌乳期。整個(gè)生理發(fā)育過程中,IGF-IR蛋白主要分布在兩組湖羊的乳腺上皮細(xì)胞和結(jié)締組織中。湖羊乳腺組織的IGF-IR蛋白表達(dá)水平變化趨勢與m RNA表達(dá)量相一致。綜上,多乳頭湖羊不僅乳腺組織較發(fā)達(dá),而且與乳腺發(fā)育相關(guān)的激素受體基因轉(zhuǎn)錄水平和蛋白質(zhì)表達(dá)量均較高。本研究結(jié)果為培育高產(chǎn)湖羊品種提供理論支撐。
[Abstract]:In order to find out whether there were differences in breast tissue structure and insulin-like growth factor-I receptor IGF-IRR between the two nipple lake sheep (control group), and whether it was possible to have higher lactation performance. In this study, the morphological changes of breast tissue were observed after HE staining of breast tissue samples from two groups of Hu sheep during the growing period, pregnancy period, lactation period and empty pregnancy stage. The expression of m RNA in breast tissue was detected by RT-PCR in different physiological stages. Immunohistochemical method (IHC) was used to detect the distribution of hormone receptor protein in mammary gland. Western blot method was used to detect the expression of hormone receptor protein. The results showed that the breast stroma of the two groups increased rapidly, the glands contained a lot of connective tissue and fat tissue, and the number of mammary ducts in the experimental group was denser than that in the control group, and during pregnancy, a large number of lobular acinus developed completely. The acinus cavity is filled with secretions and the number of acinus is more than that of the control group. During lactation, the size and shape of the acinus in the two groups increase, and the acinar cavity contains abundant milk. After weaning, the mammary acinus of the two groups gradually collapses. The morphological structure changes. The expression of IGF-IR m RNA and protein in breast tissues of the two groups increased gradually from the stage of development to lactation, while the expression of IGF-IR m RNA in the breast tissues of the control group increased gradually from the period of gestation to the period of gestation, and the expression of IGF-IR m RNA in the breast tissues of the two groups was down-regulated slightly than that in the period of gestation. After weaning, the expression of m RNA and protein distribution were lower than those in lactation stage. IGF-IR protein was mainly distributed in breast epithelial cells and connective tissues of two groups of Hu sheep. The change trend of IGF-IR protein expression in the breast tissue of Hu sheep was consistent with that of m RNA expression. In conclusion, polynipple lake sheep not only has more developed mammary tissue, but also has higher transcription level and protein expression of hormone receptor gene related to breast development. The results of this study provide theoretical support for the cultivation of high-yielding Hu sheep breeds.
【作者單位】： 西北農(nóng)林科技大學(xué)動(dòng)物科技學(xué)院;甘肅省金昌市畜牧技術(shù)推廣服務(wù)中心;陜西省靖邊縣生產(chǎn)力促進(jìn)中心;
【基金】：基金項(xiàng)目:良種羊快速繁育技術(shù)集成創(chuàng)新與良種核心群組建(No.2013KTZB02-02-01)
【分類號(hào)】：S826

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