本文選題：融合肽hEGF-AWRK6 + 肉雞��； 參考：《遼寧大學》2017年碩士論文

【摘要】：抗菌肽(antibacterial peptides,ABP)是生物體內產生的一種具有廣譜抗菌作用的小分子多肽,屬非特異性防疫系統的重要組份。人表皮生長因子(hEGF)作為促生長因子家族成員之一,具有促進新血管形成和表皮細胞增殖的作用,是上皮細胞、表皮細胞和間充質等敏感細胞的絲裂原,具有促進生長和提高免疫的作用�？咕腁WRK6是經Dybowskin-2CDYa設計改造而得的新型抗菌肽,與以往發(fā)現的其它蛙類抗菌肽相比相似性小,它富含賴氨酸,具有親水性強,穩(wěn)定性高,抑菌活性強和抑菌譜廣等特點。hEGF-AWRK6是由本實驗室將人表皮生長因子hEGF和抗菌肽AWRK6的基因DNA連接、轉化并表達純化得到的重組融合多肽。本實驗以肉雞為實驗動物,分析了融合肽hEGF-AWRK6對肉雞生產性能、養(yǎng)分代謝、抗氧化和免疫機能的影響。探討了融合肽hEGF-AWRK6替代飼用抗生素應用于家畜養(yǎng)殖的可行性。實驗選取84只1日齡健康AA肉仔雞,隨機分4組。對照組飼喂基礎日糧,實驗Ⅰ組、Ⅱ組和抗生素組分別在基礎日糧中添加50mg/kg、100mg/kg的融合肽hEGF-AWRK6和5mg/kg的黃霉素,實驗周期為42d。結果顯示:1、與對照組相比,實驗Ⅱ組與抗生素組的肉雞體重分別提高了21.4%(p0.01)和19.0%(p0.05),半凈膛、全凈膛和胸肌重亦顯著增加,且實驗Ⅱ組優(yōu)于抗生素組,而各組肉雞的全凈膛率、半凈膛率以及腿肌重沒有顯著差異;2、實驗Ⅱ組肉雞免疫器官指數、脾臟和巨噬細胞細胞活力及Sig-A水平,與對照組相比分別提高了66.67%,27.27%,68.29%和54.11%,差異顯著,且實驗Ⅱ組優(yōu)于抗生素組。3、與對照組相比,實驗Ⅱ組的肉雞肝臟的GSH-PX、T-AOC和SOD水平分別提高了的23.07%、64.6%、10.81%,并且降低MDA水平的28.57%,差異顯著。4、融合肽hEGF-AWRK6能夠顯著提高肉雞肌肉蛋白質和脂肪得含量,與對照組相比,實驗Ⅱ組的肌肉蛋白質和脂肪分別提高了15.89%(p0.01)和29.31%(p0.01)。此外,融合肽hEGF-AWRK6可提高肉雞小腸絨毛高度和隱窩深度比值,降低肉雞腸道中有害菌群大腸桿菌的數量。本文研究結論:在日料糧中添加100mg/kghEGF-AWRK6可以顯著增加肉雞的日增重,提高飼料的轉化效率,提高肉雞肌肉中蛋白和脂肪的含量;融合肽hEGF-AWRK6可提高肉雞血清中的免疫球蛋白A(Sig-A)水平,并能顯著提高肉雞的肝臟抗氧化機能,增強肉雞的抵抗力;融合肽hEGF-AWRK6可改善肉雞腸道菌群和腸道結構,降低腸道中的大腸桿菌數量;日料中添加高劑量hEGF-AWRK6(100mg/kg)組的肉雞的生產性能及免疫能力優(yōu)于日料中添加抗生素組的肉雞。本實驗為hEGF-AWRK6作為飼用抗生素替代品在家畜飼養(yǎng)中的應用提供了實驗依據。
[Abstract]:Antimicrobial peptide (ABP) is a kind of small molecular peptide with broad-spectrum antimicrobial activity produced in vivo, which is an important component of nonspecific epidemic prevention system. Human epidermal growth factor hEGF, as a member of the growth promoting factor family, is a mitogen of epithelial cells, epidermal cells and mesenchymal cells, which can promote the formation of new blood vessels and the proliferation of epidermal cells. It has the function of promoting growth and improving immunity. Antimicrobial peptide AWRK6 is a new antimicrobial peptide designed and modified by Dybowskin-2CDYa. It has less similarity with other frog antimicrobial peptides found in the past. It is rich in lysine, has strong hydrophilicity and high stability. HEGF-AWRK6 is a recombinant fusion polypeptide which is expressed and expressed by the DNA of human epidermal growth factor (hEGF) and antimicrobial peptide AWRK6 (AWRK6), which is characterized by strong bacteriostatic activity and wide antimicrobial spectrum. The effects of fusion peptide hEGF-AWRK6 on performance, nutrient metabolism, antioxidation and immune function of broilers were studied. The feasibility of using fusion peptide hEGF-AWRK6 instead of feeding antibiotics in livestock breeding was discussed. Eighty-four 1-day-old AA broilers were randomly divided into 4 groups. The control group was fed with basal diet, experimental group 鈪，

本文編號：1881881