本文選題：禽網(wǎng)狀內(nèi)皮組織增生病病毒 + SPF雛雞��； 參考：《東北農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：禽網(wǎng)狀內(nèi)皮組織增生病(Reticuloendotheliosis,RE)是由禽網(wǎng)狀內(nèi)皮組織增生病病毒(Reticuloendotheliosis virus,REV)引起的免疫抑制性腫瘤病,該病嚴(yán)重危害養(yǎng)禽業(yè)的發(fā)展。本試驗(yàn)以1日齡SPF雛雞為研究對(duì)象,應(yīng)用間接ELISA及實(shí)時(shí)熒光定量PCR方法檢測(cè)了REV感染雛雞免疫器官(胸腺、脾臟、法氏囊)過(guò)氧化氫(H2O2)、丙二醛(Malondialdehyde,MDA)含量,總抗氧化能力(Total-antioxidante capacity,T-AOC),超氧化物歧化酶(Superoxide dismutase,SOD)活力,過(guò)氧化氫酶(Catalase,CAT)、谷胱甘肽過(guò)氧化物酶1(Glutathione peroxidase 1,GPx1)含量及其mRNA表達(dá)的動(dòng)態(tài)變化,旨在從病毒感染后氧化-抗氧化功能變化的角度闡述REV致病機(jī)制,為RE的防治提供科學(xué)依據(jù)。試驗(yàn)結(jié)果發(fā)現(xiàn):(1)感染REV后,SPF雛雞免疫器官H2O2、MDA含量不同程度高于對(duì)照雛雞。其中,胸腺、脾臟、法氏囊H2O2含量分別于感染后第35 d、第28 d、第21 d顯著高于對(duì)照雛雞(P0.05);MDA含量分別于感染后第28 d、7 d-28 d、第21 d顯著或極顯著高于對(duì)照雛雞(P0.05或P0.01)。表明REV感染可導(dǎo)致SPF雛雞免疫器官氧化物質(zhì)增多,脂質(zhì)過(guò)氧化反應(yīng)增強(qiáng),機(jī)體處于氧化應(yīng)激。(2)感染REV后,SPF雛雞免疫器官T-AOC、SOD活力不同程度低于對(duì)照雛雞。其中,胸腺、脾臟、法氏囊T-AOC分別于感染后21 d-35 d、28 d-35 d、第35 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01);SOD活力分別于感染后14 d-28 d、14 d-21 d、21 d-35 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01)。表明REV感染可使SPF雛雞免疫器官抗氧化功能顯著降低。(3)感染REV后,SPF雛雞免疫器官CAT、GPx1含量及基因表達(dá)水平不同程度低于對(duì)照雛雞。其中,胸腺、脾臟、法氏囊CAT含量分別于感染后14 d-28 d、28 d-35 d、第21 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01);胸腺、脾臟、法氏囊GPx1含量分別于感染后第28 d、14 d-21 d、第14 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01);胸腺、脾臟CAT mRNA含量分別于感染后21 d-28 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01);胸腺、脾臟、法氏囊GPx1 mRNA含量分別于感染后28 d-35 d、第35 d、第28 d顯著低于對(duì)照雛雞(P0.05)。表明REV感染可導(dǎo)致免疫器官CAT、GPx1基因及蛋白表達(dá)下降,清除過(guò)氧化物能力降低,機(jī)體抗氧化酶含量減少,抗氧化功能減弱。(4)感染REV后,SPF雛雞胸腺、脾臟、法氏囊器官指數(shù)于28 d-35 d顯著或極顯著低于對(duì)照雛雞(P0.05或P0.01)。表明REV感染可使免疫器官器官指數(shù)下降,免疫器官萎縮,機(jī)體發(fā)生免疫抑制。
[Abstract]:Reticuloendotheliosissis-Reticuloendotheliosis-Reticuloendotheliosis virus (Reticuloendotheliosis virus Rev) is a kind of immunosuppressive tumor caused by reticuloendotheliosis virus (Rev), which seriously harms the development of poultry industry. The content of H _ 2O _ 2 H _ 2O _ 2, malondialdehyde (MDA) and malondialdehyde (MDA) in immune organs (thymus, spleen, bursa) of chicks infected with REV were detected by indirect ELISA and real-time fluorescence quantitative PCR. The dynamic changes of total antioxidant capacity, activity of superoxide dismutase (SOD), catalase (catalase), glutathione peroxidase (1(Glutathione peroxidase) 1GPx1) and their mRNA expression were observed. The aim of this study was to elucidate the pathogenetic mechanism of REV from the point of view of the changes of oxidation-antioxidant function after virus infection, and to provide scientific basis for the prevention and treatment of RE. The results showed that the content of MDA in the immune organ of chicken infected with REV was higher than that of the control. The H2O2 content of thymus, spleen and bursa of Fabricius was significantly higher than that of control chicks on the 35th day, the 28th day and the 21st day after infection, respectively. The content of H2O2 in the thymus, spleen and bursa of the control was significantly higher than that of the control chicks on the 28th day, the 7th day and the 21st day, respectively, and was significantly or extremely higher than that of the control chicks on the 35th day, the 28th day, the 21st day, respectively. The results showed that REV infection could lead to the increase of oxidative substances in immune organs and the enhancement of lipid peroxidation in SPF chicks, and the activity of T-AOC- SOD in immune organs of SPF chicks was lower than that of control chicks after REV infection. The T-AOC activity of thymus, spleen and bursa of Fabricius was significantly or extremely lower than that of control chicks on day 35 after infection at 21 d ~ 35 d, respectively. The activity of T-AOC in thymus, spleen and bursa of Fabricius was significantly or extremely lower than that of control chicks at 14 d ~ 28 d ~ (14 d) ~ 21 d ~ (21) d ~ (35) after infection. The results showed that REV infection could significantly decrease the antioxidant function of immune organs of SPF chicks.) after REV infection, the content of SPF GPx1 and the level of gene expression in the immune organs of SPF chicks were lower than those of control chicks. The contents of CAT in thymus, spleen and bursa of Fabricius were significantly or extremely lower than those of control chicks on the 21st day (P 0.05 or P 0.01), and the thymus, spleen and spleen were significantly lower than those of control chicks at 14 d ~ 28 d ~ (28) d ~ (35) after infection. The GPx1 content of bursa of Fabricius was significantly or extremely lower than that of control chicks on day 14 and day 21, respectively, and the CAT mRNA content of thymus and spleen was significantly or very significantly lower than that of control chicks on day 21 and 28, respectively, and the contents of thymus and spleen were significantly or extremely lower than those of control chicks at 21 d to 28 d after infection, and the thymus, spleen, thymus and spleen, respectively. The content of GPx1 mRNA in bursa of Fabricius was significantly lower than that of control chicks at 28 d, 35 d and 28 d after infection, respectively. The results showed that REV infection could decrease the expression of REV GPx1 gene and protein, decrease the ability of scavenging peroxide, decrease the content of antioxidant enzymes, and weaken the antioxidant function of REV.) after infection with REV, the thymus and spleen of REV chicks were infected. The organ index of bursa of Fabricius was significantly or extremely significantly lower than that of control chicks (P0.05 or P0.01) at 28 d ~ 35 d. The results showed that REV infection could decrease the immune organ index, decrease the immune organ atrophy and induce immunosuppression.
【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.31

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本文編號(hào)：1870313