本文選題：豬支原體肺炎 + 防治��； 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：豬支原體肺炎是對世界養(yǎng)豬業(yè)威脅最嚴(yán)重的疾病之一,在我國多個地區(qū)均有報道過豬支原體肺炎的流行與發(fā)生。石河子墾區(qū)部分規(guī)模化豬場豬支原體肺炎流行病學(xué)調(diào)查的結(jié)果(血清陽性率58.94%)顯示,該病已在墾區(qū)部分規(guī)�；i場流行,給墾區(qū)養(yǎng)豬業(yè)帶來了較大的經(jīng)濟(jì)損失。為降低該病對墾區(qū)養(yǎng)豬業(yè)的危害,進(jìn)行了應(yīng)用3號中草藥添加劑提高仔豬抗病力、監(jiān)測常用疫苗免疫接種的效果、敏感藥物體外篩選以及常用消毒劑對墾區(qū)分離株殺滅效果的檢測等防治的研究,結(jié)果如下:1、3號中草藥添加劑對斷奶仔豬部分免疫指標(biāo)及增重的影響為提高斷奶仔豬抗豬支原體肺炎的能力,試驗采用3號添加劑,對仔豬進(jìn)行10天飼喂試驗后,采用ELISA方法檢測血清中Ig A、Ig G、CD4+和CD8+水平。結(jié)果:試驗前后血清中Ig A、Ig G、CD4+、CD8+和CD4+/CD8+水平增長的情況是:與對照組相比,健康試驗組分別增長了8.23%(P0.05)、37.58%(0.01P0.05)、48.04%(P0.05)、82.23%(P0.05)和11.63%(P0.05);病弱試驗組分別增長了282.30%(P0.05)、276.66%(P0.05)、322.97%(0.01P0.05)、169.38%(0.01P0.05)和172.61%(P0.05);健康豬和病弱豬的日增重分別增加了20.06%(P0.05)和133.33%(P0.01)。因此,3號中草藥添加劑能有效地提高斷奶仔豬的部分免疫指標(biāo)和生長性能。2、三種豬肺炎支原體滅活疫苗免疫效果的比較為了解墾區(qū)豬場使用量較大的A、B、C三種豬肺炎支原體滅活苗的免疫效果,試驗選擇10日齡仔豬進(jìn)行了免疫效果對比試驗,分別于10日齡和32日齡進(jìn)行免疫,于首免前、二免前和二免后21天(55日齡)采集血清,用ELISA試劑盒檢測抗體效價和抗體陽性率。結(jié)果:仔豬血清中豬肺炎支原體抗體水平都呈先下降后上升的趨勢,55日齡時B組的豬肺炎支原體抗體水平和抗體陽性率均最高,分別為0.887和94.7%,其次是A組(0.347和68.2%)、C組(0.094和12.6%)和對照組(0.006和5.6%)。結(jié)論:B疫苗對仔豬的免疫保護(hù)效果優(yōu)于A疫苗和C疫苗。3、墾區(qū)豬肺炎支原體分離株的體外藥敏試驗為篩選出對墾區(qū)豬場豬肺炎支原體敏感的藥物,降低豬支原體肺炎對墾區(qū)養(yǎng)豬業(yè)造成的損失,試驗選取氟苯尼考、恩諾沙星、環(huán)丙沙星等12種常用抗生素或抗生素聯(lián)合進(jìn)行了體外藥敏試驗。結(jié)果:墾區(qū)三株分離株均對泰樂菌素、替米考星和泰妙菌素敏感;對鹽酸環(huán)丙沙星+安乃近、泰妙菌素+土霉素、氟苯尼考等藥物敏感性較差;對恩諾沙星、鹽酸環(huán)丙沙星、土霉素等不敏感,有耐藥性。4、墾區(qū)豬肺炎支原體分離株的消毒劑篩選試驗為篩選出能有效殺滅墾區(qū)豬肺炎支原體分離株的消毒劑,降低墾區(qū)豬場豬肺炎支原體的感染率,試驗選擇聚維酮碘、月芐三甲氯胺、復(fù)合酚和戊二醛四種消毒劑,對墾區(qū)分離株進(jìn)行了殺菌效果試驗。結(jié)果:對聚維酮碘,JX株和SY株高度敏感,TL株不敏感;對月芐三甲氯胺,SY株高度敏感,JX株和TL株不敏感;對復(fù)合酚,SY株高度敏感,JX株中度敏感,TL株不敏感;對戊二醛,三株分離株均高度敏感。結(jié)論:墾區(qū)豬肺炎支原體分離株對戊二醛和聚維酮碘較敏感,其次是月芐三甲氯胺和復(fù)合酚。
[Abstract]:Mycoplasma porcine pneumonia is one of the most serious diseases that threaten the pig industry in the world. The prevalence and occurrence of Mycoplasma pneumoniae have been reported in many regions of China. The epidemiological investigation of mycoplasma pneumonia in some large-scale pig farms in Shihezi reclamation area (seropositive rate 58.94%) shows that the disease has been prevalent in some large-scale pig farms in the reclamation area. In order to reduce the harm to the pig industry in the reclamation area, to reduce the harm of the disease to the pig industry in the reclamation area, the application of No. 3 Chinese herbal medicine additive to improve the resistance of piglets, the effect of monitoring the immunization of common vaccines, the screening of sensitive drugs in vitro and the prevention and control of the killing effect of the common disinfectants on the isolated strains of the reclamation area were studied. The results are as follows: the effect of 1,3 Chinese herbal additive on the immune index and weight gain of weanling piglets is to improve the ability of weaning piglets to resist Mycoplasma pneumoniae pneumonia. The experiment adopted No. 3 additive. After feeding the piglets for 10 days, the serum level of Ig A, Ig G, CD4+ and CD8+ were detected by ELISA method. The level of 4+, CD8+ and CD4+/CD8+ increased: compared with the control group, the health test group increased by 8.23% (P0.05), 37.58% (0.01P0.05), 48.04% (P0.05), 82.23% (P0.05) and 11.63% (P0.05); the weak test group increased by 282.30% (P0.05), 276.66% (P0.05), 322.97% (0.01P0.05), 169.38% (0.01P0.05) and 172.61%, and healthy pigs and diseases. The daily weight gain of the weak pigs increased by 20.06% (P0.05) and 133.33% (P0.01) respectively. Therefore, the Chinese herbal additive No. 3 could effectively improve the immune index and growth performance.2 of weaned piglets, and the comparison of the immune effect of three kinds of Mycoplasma pneumoniae inactivated vaccine in order to solve the inactivated vaccine of A, B, C and three kinds of Mycoplasma pneumoniae in the pig farm of the reclamation area. Immune effect, the test selected 10 days old piglets to carry out a comparative test of immune effect, respectively at 10 days of age and 32 days of age, respectively, before the first exemption, two before and 21 days after two exempts (55 days of age) to collect serum, ELISA kit was used to detect antibody titer and antibody positive rate. Results: the antibody level of Mycoplasma porcine Mycoplasma in the serum of piglets decreased first. After 55 days of age, the antibody level and antibody positive rate of Mycoplasma pneumoniae in group B were the highest, 0.887 and 94.7%, followed by group A (0.347 and 68.2%), group C (0.094 and 12.6%) and control group (0.006 and 5.6%). Conclusion: the immune protection effect of B vaccine to piglets was better than A vaccine and C vaccine.3, and the Mycoplasma pneumoniae isolates were isolated in the reclamation area. In vitro drug sensitivity test was used to screen out the drug sensitive to Mycoplasma pneumoniae in the pig farm and reduce the loss of mycoplasma pneumonia to pig industry in the reclamation area. The test selected 12 common antibiotics or antibiotics, including florfenicol, enrofloxacin, ciprofloxacin and other antibiotics in vitro. The results showed that three strains of isolated strains in the reclamation area were all of tylosa. The sensitivity of timoxicin and thomikacin, ciprofloxacin + antidol, teiericin + oxytetracycline, florfenicol and other drugs were poor, insensitive to enrofloxacin, ciprofloxacin hydrochloride and oxytetracycline, and drug resistance.4, and the screening test of the disinfectant of Mycoplasma pneumoniae isolates in the reclamation area could effectively kill the pig lung in the reclamation area. The disinfectant of Mycoplasma Mycoplasma isolates reduced the infection rate of Mycoplasma pneumoniae in the pig farm of the reclamation area. The experiment selected four kinds of disinfectants, including polyvidone iodine, trimethylamine chloramine, compound phenol and glutaraldehyde, and tested the germicidal efficacy of the isolated strains in the reclamation area. Results: polyvidone iodine, JX strain and SY strain were highly sensitive, TL strain was insensitive; against trimethylamine trimetholamine, SY The plant was highly sensitive, JX strain and TL strain were insensitive; SY strain was highly sensitive to compound phenol, SY strain, JX strain was moderately sensitive, TL strain was insensitive, and glutaraldehyde and three isolates were highly sensitive. Conclusion: the isolates of Mycoplasma pneumoniae were sensitive to glutaraldehyde and povidone iodine, followed by benzyl trifolamine and compound phenol.

【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28

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