本文選題：甲狀腺激素 + 抗氧化狀態(tài)　； 參考：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：甲狀腺激素(thyroid hormones)和氧化應(yīng)激(oxidative stress)在雌性動(dòng)物生殖系統(tǒng)的正常生理功能中發(fā)揮著重要作用。一氧化氮(nitric oxide,NO)是一種由一氧化氮合酶(nitric oxide synthases,NOS)催化合成的小分子自由基,可參與甲狀腺功能和氧化應(yīng)激狀態(tài)的調(diào)節(jié),也被認(rèn)為是一個(gè)評(píng)價(jià)氧化應(yīng)激狀態(tài)的標(biāo)記物。本研究的目的是研究甲狀腺激素對(duì)青年大鼠卵巢和子宮氧化應(yīng)激的影響。30只剛斷奶的未成熟SD大鼠被隨機(jī)分成三個(gè)組：對(duì)照組(Control),甲狀腺功能減退(甲減)組(Hypo-T),和甲狀腺功能亢進(jìn)(甲究)組(Hyper-T)。使用丙硫氧密啶(propylthiouracil,PTU)和甲狀腺激素(thyronine,T4)來(lái)分別構(gòu)建甲減和甲亢大鼠模型。研究結(jié)果顯示:甲減組和甲亢組大鼠的體重和卵巢重量都會(huì)顯著降低,甲減組的子宮重量顯著降低。甲減組和甲亢組卵巢中的卵泡數(shù)量也顯著減少。血液中三磺甲狀腺原氨酸(triiodothyronine,T3),甲狀腺素(thyroxine,T4)和雌二醇(estradiol,E2)的濃度在甲減組大鼠中顯著降低,而在甲亢組大鼠中會(huì)顯著升高。此外,甲減組和甲亢組大鼠血液中的孕酮(progesterone,P4)濃度都顯著降低。免疫組織化學(xué)結(jié)果表明:甲狀腺激素受體(TRα/β)和三種NOS(nNOS、iNOS和eNOS)在大鼠卵巢和子宮的不同類型細(xì)胞中定位和表達(dá),表明這些受體對(duì)卵巢和子宮的生長(zhǎng)發(fā)育和功能起著重要作用。卵巢和子宮中的NO含量,總NOS活性,以及誘導(dǎo)型NOS(iNOS)活性在甲減組大鼠中顯著降低,而在甲亢組大鼠中會(huì)顯著升高。卵巢中結(jié)構(gòu)型NOS(cNOS)活性在甲減組大鼠中最低,而在甲亢組和對(duì)照組大鼠之間沒(méi)有顯著差異。甲減組大鼠子宮中cNOS的活性要比甲亢組大鼠顯著降低,但甲減組和甲亢組大鼠都與對(duì)照組大鼠差異不顯著。卵巢和子宮中的丙二醛(malondialdehyde,MDA)含量在甲減組和甲亢組大鼠中都升高。卵巢和子宮中的過(guò)氧化氫酶(catalase,CAT)和谷胱甘肽過(guò)氧化物酶(glutathioneperoxidase,GSH-PX)的活性在甲減組大鼠中降低,而在甲亢組大鼠中都升高。卵巢中總超氧化物歧化酶(totalsuperoxide dismutase,T-SOD)的活性在甲亢組大鼠中最高,而在甲減組和對(duì)照組大鼠之間沒(méi)有顯著差異。子宮中的T-SOD的活性在甲減組和甲亢組大鼠中都比對(duì)照組顯著降低,但在甲減組和甲亢組大鼠之間沒(méi)有顯著差異。綜上所述,通過(guò)使用甲減甲亢大鼠模型進(jìn)行研究,實(shí)驗(yàn)結(jié)果提供了甲狀腺功能紊亂對(duì)青年大鼠卵巢和子宮中氧化應(yīng)激有重要影響的新證據(jù)。但是,在甲狀腺激素引起的生殖疾病中,NOS信號(hào)和氧化應(yīng)激的作用仍有待進(jìn)一步研究。值得注意的是,甲狀腺激素與雌性生殖系統(tǒng)的抗氧化狀態(tài)有重要聯(lián)系,這些結(jié)果也有助于闡明甲狀腺功能紊亂對(duì)雌性動(dòng)物生殖的影響。
[Abstract]:Thyroid hormone thyroid hormones (thyroid hormones) and oxidative stress stress (oxidative stress) play an important role in the normal physiological function of female reproductive system. Nitric oxide (no) is a small molecular free radical catalyzed by nitric oxide synthase (oxide syntheses), which is involved in the regulation of thyroid function and oxidative stress state, and is also considered to be a marker for evaluating oxidative stress state. The purpose of this study was to study the effects of thyroid hormone on oxidative stress in ovary and uterus of young rats. Thirty newly weaned immature SD rats were randomly divided into three groups: control group (control group), hypothyroidism (hypothyroidism) group, and hypothyroidism (hypothyroidism) group. Hyperthyroidism group (Hyper-T) group. Rat models of hypothyroidism and hyperthyroidism were established by using propylthiouraciline (PTU) and thyroid hormone thyronine (T4) respectively. The results showed that the weight and ovarian weight of hypothyroidism group and hyperthyroidism group decreased significantly, while the uterine weight of hypothyroidism group decreased significantly. The number of follicles in hypothyroidism and hyperthyroidism was also significantly reduced. The concentrations of triiodothyronine (T3), thyroxine (T4) and estradiolium (E2) in blood decreased significantly in hypothyroidism group, but increased significantly in hyperthyroidism group. In addition, the serum progesterone P4 concentration in hypothyroidism group and hyperthyroidism group was significantly decreased. Immunohistochemical results showed that thyroid hormone receptor tr 偽 / 尾 and three NOSnNOSiNOS and Enos) were localized and expressed in different types of cells of rat ovary and uterus, indicating that these receptors play an important role in the growth, development and function of ovary and uterus. No content, total NOS activity and inducible NOSI-NOSs activity in ovary and uterus decreased significantly in hypothyroidism group, but increased in hyperthyroidism group. The activity of structural NOSc NOSs in ovary was the lowest in hypothyroidism group, but there was no significant difference between hyperthyroidism group and control group. The activity of cNOS in hypothyroidism group was significantly lower than that in hyperthyroidism group, but there was no significant difference between hypothyroidism group and hyperthyroidism group and control group. The content of malondialdehyde (MDA) in ovary and uterus was increased in hypothyroidism group and hyperthyroidism group. The activities of catalase (CAT) and glutathione peroxidase (glutathione peroxidase) in ovary and uterus decreased in hypothyroidism group and increased in hyperthyroidism group. The activity of total superoxide dismutase (T-SOD) in ovary was the highest in hyperthyroidism group, but there was no significant difference between hypothyroidism group and control group. The activity of T-SOD in uterus was significantly lower in hypothyroidism group and hyperthyroidism group than in control group, but there was no significant difference between hypothyroidism group and hyperthyroidism group. In conclusion, by using hypothyroidism rat model, the results provide new evidence that thyroid dysfunction has an important effect on oxidative stress in ovary and uterus of young rats. However, the role of nitric oxide synthase (NOS) and oxidative stress in thyroid hormone-induced reproductive diseases remains to be further studied. It is important to note that thyroid hormones are closely related to the antioxidant status of the female reproductive system. These results also help to clarify the effects of thyroid dysfunction on the reproduction of female animals.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S856.5

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