本文選題：豬滲出性皮炎 + 豬葡萄球菌　； 參考：《中國獸醫(yī)科學(xué)》2017年02期

【摘要】：為了解患滲出性皮炎仔豬中豬葡萄球菌內(nèi)表皮脫落素的流行情況,采集2個豬場(A場和B場)29頭患病豬的痂皮、痂皮下滲出液共48份,進行了豬葡萄球菌的分離鑒定及其脫落素基因的檢測。結(jié)果顯示,48份樣品中共分離到29株含脫落素基因的葡萄球菌,經(jīng)生化鑒定和Gap基因測序分析,表明所有菌株均屬于豬葡萄球菌。其中,A場和B場分離的豬葡萄球菌脫落素檢出率分別為93.3%和64.3%;A場可檢出Exh A、Exh C、Exh D和Shet A共4種脫落素基因,B場可檢出Exh A、Exh D和Shet A共3種脫落素基因,但Shet A的檢出率在2個豬場中均為最高且與其他3種脫落素大量共存,共存率在A場和B場分別為61.5%和70.0%。2個豬場中痂皮源葡萄球菌中脫落素檢出率均高于皮下滲出液,脫落素與滲出性皮炎病具有較高的相關(guān)性,其檢出率受到2個豬場不同的飼養(yǎng)環(huán)境和采樣部位的影響,Shet A的高檢出率與其他脫落素共存的情況,提示該毒素可能廣泛參與了豬葡萄球菌的致病作用。
[Abstract]:In order to understand the prevalence of epidermal abscisic hormone in swine staphylococcus in piglets suffering from exudative dermatitis, 48 samples of epidermal exudate were collected from 29 infected pigs in two pig farms (field A and B). Isolation and identification of porcine staphylococcus and detection of abscisic gene were carried out. The results showed that 29 strains of Staphylococcus containing abscisic gene were isolated from 48 samples. Biochemical identification and Gap gene sequencing showed that all strains belonged to staphylococcus porcine. The detectable rate of abscisic hormone isolated from field A and field B was 93.3% and 64.3%, respectively. Four abscisic genes (Exh AhD and Shet A) were detected in field A and Shet A, respectively. But the detection rate of Shet A was the highest in two pig farms and coexisted with the other three abscisic substances. The coexisting rate was 61.5% in field A and 70.0.2% in field B, respectively. The detection rate of abscisic in Staphylococcus callus was higher than that in subcutaneous exudate. There was a high correlation between abscisic hormone and exudative dermatitis. The positive rate of shet A was affected by the different feeding environment and sampling site in two pig farms, and the high rate of Shet A coexisted with other exudative dermatitis. These results suggest that the toxin may be involved in the pathogenicity of Staphylococcus suis.
【作者單位】： 湖南農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院;
【基金】：湖南省戰(zhàn)略性新興產(chǎn)業(yè)科技攻關(guān)類項目(2016GK4015)
【分類號】：S858.28

【相似文獻】

相關(guān)期刊論文 前2條

1 沈孟生;;沼氣綜合利用技術(shù)講座(連載之二)[J];農(nóng)村實用工程技術(shù);1991年04期

2 ;[J];;年期

，

本文編號：1837744