本文選題：BMPR-IB基因 + 山羊�。� 參考：《中國畜牧獸醫(yī)》2017年05期

【摘要】：試驗旨在利用生物信息學方法分析山羊骨形態(tài)發(fā)生蛋白受體IB(bone morphogenetic protein receptor IB,BMPR-IB)基因編碼蛋白的結(jié)構(gòu)及功能。本研究通過在線軟件預(yù)測和分析山羊BMPR-IB基因編碼蛋白的一級結(jié)構(gòu)、二級結(jié)構(gòu)、亞細胞定位、三級結(jié)構(gòu)、重要的功能基序及其分類,通過最大似然法對不同物種BMPR-IB基因編碼蛋白序列構(gòu)建系統(tǒng)發(fā)育樹,進行系統(tǒng)發(fā)育分析。結(jié)果發(fā)現(xiàn),山羊BMPR-IB基因共編碼502個氨基酸,其編碼蛋白屬于不穩(wěn)定的親水性蛋白質(zhì)。二級結(jié)構(gòu)以β折疊為主,比例為63.5%,主要存在于細胞核中,在線粒體、細胞質(zhì)、囊泡分泌系統(tǒng)和質(zhì)膜中也有少量分布。該蛋白主要發(fā)揮嘌呤和嘧啶、監(jiān)管、運輸和結(jié)合、信號轉(zhuǎn)導等功能。在127-149氨基酸位置存在1個跨膜結(jié)構(gòu)域;在174-203、204-494氨基酸位置存在2個高度保守的基序,分別為GS結(jié)構(gòu)域和蛋白激酶結(jié)構(gòu)域。三級結(jié)構(gòu)由sheet片段結(jié)構(gòu)富集域和helix螺旋結(jié)構(gòu)富集域組成。不同物種BMPR-IB基因系統(tǒng)發(fā)育分析結(jié)果與動物學分類結(jié)果一致,提示BMPR-IB基因與繁殖力性狀存在聯(lián)系。通過不同的在線預(yù)測軟件分析了山羊BMPR-IB基因編碼蛋白的結(jié)構(gòu)及功能,為深入研究BMPR-IB基因提供理論指導。
[Abstract]:The purpose of this study was to analyze the structure and function of the gene encoding protein of goat bone morphogenetic protein receptor IB (bone morphogenetic protein receptor IB, BMPR-IB) by bioinformatics. This study predicted and analyzed the first class structure, two stage structure, subcellular localization, three grade structure and weight of goat BMPR-IB gene encoding protein by on-line software. The phylogenetic tree of the BMPR-IB gene encoding protein sequences of different species was constructed by the maximum likelihood sequence and the phylogenetic analysis. The results showed that the BMPR-IB gene of goats encodes 502 amino acids, and the encoding protein belongs to the unstable hydrophilic egg white. The two grade structure is mainly beta fold, and the proportion is 63.5%. The protein mainly exists in the nucleus, in the mitochondria, the cytoplasm, the vesicle secretory system and the plasma membrane. The protein mainly plays the functions of purine and pyrimidine, regulation, transport and binding, signal transduction. There are 1 transmembrane domains in the 127-149 amino acid position; there are 2 highly conserved sequences in the 174-203204-494 amino acid position, GS domain and protein kinase domain respectively. The three stage structure is composed of the sheet fragment structure enrichment domain and the helix spiral structure enrichment domain. The phylogenetic analysis results of different species BMPR-IB genes are in agreement with the zoological classification results, suggesting that the BMPR-IB gene is associated with the reproductive traits. The structure and function of the encoded protein of sheep BMPR-IB gene provide theoretical guidance for further study of BMPR-IB gene.

【作者單位】： 河北農(nóng)業(yè)大學動物科技學院;
【基金】：國家現(xiàn)代肉羊產(chǎn)業(yè)技術(shù)體系項目(CARS-39) 河北農(nóng)業(yè)大學青年科學基金項目(QJ201218)
【分類號】：S827
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本文編號：1832056