本文選題：血清型禽腺病毒 + 心包積液��； 參考：《中國獸醫(yī)學(xué)報(bào)》2017年06期

【摘要】：山東、河北等華北地區(qū)12個(gè)肉雞養(yǎng)殖場出現(xiàn)了以心包積液、出血性肝炎為特征的疾病,從采集的病料中進(jìn)行病毒分離鑒定。本研究對分離的毒株進(jìn)行PCR擴(kuò)增、血凝試驗(yàn)、ELD_(50)測定、Hexon基因序列測定與分析以及人工感染試驗(yàn)進(jìn)行組織病理學(xué)觀察、抗體水平檢測等一系列試驗(yàn)。結(jié)果顯示,分離的毒株不能凝集雞和鴨的紅細(xì)胞,但能夠凝集大鼠的紅細(xì)胞,ELD_(50)為10-3.17/0.2mL。組織學(xué)變化顯示肝細(xì)胞纖維化和脂肪變性,腎小管上皮細(xì)胞腫脹、變性,心肌纖維水腫、顆粒變性。對臨床采集的血清檢測結(jié)果顯示發(fā)病雞群抗體水平為陽性,發(fā)病雞群中的健康雞陽性率為40%(40/100),表明雞群中存在隱性感染。將分離株的Hexon基因組與已發(fā)表的血清4型禽腺病毒基因進(jìn)行同源性比較,發(fā)現(xiàn)該分離株與印度株在同一個(gè)分支上,同源性在99.6%以上,而與韓國、歐洲、美洲株同源性較遠(yuǎn)。用分離的毒株感染15日齡的雛雞能引起心包積液、肝臟出血、腎臟腫大等病變。上述結(jié)果表明該分離株為血清4型禽腺病毒。
[Abstract]:A disease characterized by pericardial effusion and hemorrhagic hepatitis was found in 12 poultry farms in North China such as Shandong and Hebei. The virus was isolated and identified from the collected samples. In this study, the isolated strains were amplified by PCR, the Hexon gene sequence was determined by hemagglutination test (ELDL / 50), and a series of tests were carried out, such as histopathological observation, antibody level detection and so on. The results showed that the isolated strains could not agglutinate the erythrocytes of chickens and ducks, but the erythrocytes of rats could be agglutinated by ELDX 50) (10-3.17 / 0.2mL). Histological changes showed hepatic fibrosis and fatty degeneration, tubule epithelial cells swelling, degeneration, myocardial fiber edema, granular degeneration. The results of clinical serum test showed that the antibody level was positive and the positive rate of healthy chickens was 40% 100%, which indicated that there was recessive infection in chickens. The Hexon genome of the isolated strain was compared with the published serotype 4 avian adenovirus gene. It was found that the homology of the isolated strain was over 99.6% in the same branch with the Indian strain, but far from that of the Korean, European and American strains. 15-day-old chickens infected with isolated strains of virus can cause pericardial effusion, liver hemorrhage, renal enlargement and so on. The results indicated that the strain was serotype 4 avian adenovirus.
【作者單位】： 山東農(nóng)業(yè)大學(xué)動物科技學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31272583,31472199)
【分類號】：S858.31

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本文編號：1824754