本文選題：釀酒葡萄皮渣 + 羔羊　； 參考：《畜牧獸醫(yī)學(xué)報》2017年09期

【摘要】：旨在研究日糧中添加釀酒葡萄皮渣(WGP)對綿羊瘤胃代謝及發(fā)育的影響。試驗隨機選取24只杜泊×小尾寒羊雜交一代公羔((25±1)kg,5月齡),采用單因素完全隨機設(shè)計平均分為4組,分別飼喂不同WGP添加水平日糧(0%,5%,10%,20%),試驗期80d。試驗結(jié)束后,屠宰并取樣,分別稱量各胃室重量;采集瘤胃液及瘤胃組織,研究瘤胃發(fā)酵參數(shù)、微生物酶活性及瘤胃上皮組織發(fā)育情況。結(jié)果表明:1)日糧添加WGP對綿羊瘤胃液pH及總揮發(fā)性脂肪酸(TVFA)濃度影響不顯著(P0.05),但顯著提高丙酸摩爾比,降低乙酸摩爾比、乙丙比及乳酸、氨態(tài)氮、尿素氮濃度(P0.05)。2)淀粉酶、蛋白酶及各纖維素酶(除果膠酶)活性均隨WGP添加水平升高而顯著下降(P0.05),果膠酶活性表現(xiàn)為10%組0%組5%組20%組(P0.05),瘤胃液總蛋白濃度在各處理間無顯著差異(P0.05)。3)VFA吸收與代謝相關(guān)的因子中,MCT1和AE2mRNA相對表達(dá)量隨WGP添加而顯著下降(P0.05),而MCT4與Na~+-K~+-ATPase在各處理之間無顯著差異(P0.05)。4)日糧添加WGP對瘤胃絕對重量及相對重量均無顯著影響(P0.05)。此外,WGP的添加可顯著降低角質(zhì)層厚度,20%添加組瘤胃乳頭長度顯著低于其他組(P0.05)。綜上,綿羊日糧中添加WGP可在一定范圍內(nèi)改變瘤胃代謝,有利于瘤胃發(fā)育。從瘤胃上皮組織發(fā)育來看,WGP添加量不宜超過10%。
[Abstract]:The purpose of this study was to study the effect of adding wine grape skin residue (WGP) into diet on rumen metabolism and development of sheep. A total of 24 male lambs were randomly divided into 4 groups by using a single factor complete randomized design. They were fed with different WGP supplementation levels in a diet of 10 ~ 10 ~ (20) and 20 ~ (th) ~ 10 ~ (th) ~ (th), and the trial period was 80 days. After the experiment, slaughtering and sampling, weighing the weight of each stomach chamber, collecting rumen fluid and rumen tissue, studying rumen fermentation parameters, microbial enzyme activity and rumen epithelium development. The results showed that dietary WGP supplementation had no significant effect on rumen pH and total volatile fatty acid (TVB) concentration in sheep, but significantly increased the molar ratio of propionic acid to acetic acid, decreased the mole ratio of acetic acid, ethylene-C and lactic acid, ammonia nitrogen and urea nitrogen (P0.05 / 2) amylase. The activities of protease and cellulase (except Pectinase) decreased significantly with the increase of WGP supplementation level. The P0. 05% group of 10% group and 20% group of 20% group of 10% group showed P0. 05% P0. 05%. There was no significant difference in total protein concentration of rumen fluid among different treatments. The relative expression of MCT1 and AE2mRNA in metabolism-related factors decreased significantly with the addition of WGP, but there was no significant difference between MCT4 and Na-K-ATPase among the treatments. The dietary supplementation of WGP had no significant effect on the absolute weight and relative weight of rumen. In addition, the addition of WGP significantly reduced the thickness of cuticle and the length of rumen papilla in the group of 20% addition was significantly lower than that in the other groups (P 0.05). In conclusion, the supplementation of WGP in sheep diet can change rumen metabolism to a certain extent, which is beneficial to rumen development. According to the development of rumen epithelium, the addition of WGP should not exceed 10%.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動物科技學(xué)院;山西省右玉縣畜牧局;
【基金】：國家現(xiàn)代肉羊產(chǎn)業(yè)技術(shù)體系專項(CARS-39) 山西省重點研發(fā)計劃項目(201603D221026-1) 山西省協(xié)同創(chuàng)新中心項目
【分類號】：S826.5

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本文編號：1818168