本文選題：豬精液 + 稀釋液�。� 參考：《西北農(nóng)林科技大學(xué)》2015年碩士論文

【摘要】：為了研究氨基酸類保護劑(�；撬�、L-谷氨酰胺)和中藥活性成分(五味子乙素、三七皂甙R1)對豬精液常溫保存效果的影響,首先設(shè)計4種稀釋液配方,檢測其保存過程中精子活率、有效保存時間、質(zhì)膜完整率、頂體完整率,篩選出保存效果最好的基礎(chǔ)配方。然后在基礎(chǔ)配方中添加分別添加0.5、1、5、10 mmol/L的�；撬�(Tau),0.5、1、2、4、8、16 mmo/L的L-谷氨酰胺(Gln),1、5、10、15μmol/L的五味子乙素(Sch B)和0.5、1、2、4、8、16 mmo/L的L-谷氨酰胺(Gln),設(shè)置空白對照組,檢測精液品質(zhì)及總抗氧化能力(T-AOC)和丙二醛(MDA)濃度,分析對豬精液保存效果的影響,確定最適添加量,旨在開發(fā)新型豬精液常溫保存的稀釋粉。本研究主要獲得如下結(jié)果:1.在4種自配豬精液常溫保存稀釋液中,“稀釋液3”,主要成分是葡萄糖40 g/L,檸檬酸鈉6.8 g/L,碳酸氫鈉0.8 g/L,EDTA1.5 g/L,檸檬酸0.3 g/L,氯化鉀0.7 g/L,有效保存時間顯著高于其他3種稀釋液(P0.05),有效保存時間為4.4 d,保存第4 d的精子活率為0.61,質(zhì)膜完整率是57.32%,頂體完整率是78.12%;其次是“稀釋液2”和“稀釋液4”,保存效果最差的是“稀釋液1”。因此,“稀釋液3”是4種自配豬精液常溫保存稀釋液中保存效果最好的稀釋液配方,可作為基礎(chǔ)稀釋液。2.在基礎(chǔ)稀釋液“稀釋液3”中分別添加不同濃度的牛磺酸(Tau)和L-谷氨酰胺(Gln),表明添加5 mmol/L的Tau時,精子活率、頂體完整率、總抗氧化能力(T-AOC)濃度顯著高于其他組(P0.05),丙二醛(MDA)濃度顯著低于其他組(P0.05),保存第5 d時,精子活率為0.66、頂體完整率47.48%、質(zhì)膜完整率為72.10%、T-AOC濃度4.28 IU/mL、MDA濃度1.63 nmol/mL。添加4 mmol/L的Gln,精子活率、質(zhì)膜完整率和頂體完整率顯著高于其他組(P0.05),保存第5 d時,精子活率為0.59、質(zhì)膜完整率47.05%、頂體完整率為74.40%、T-AOC濃度4.34 IU/mL、MDA濃度1.51 nmol/mL;添加16 mmol/L的Gln,精子活率、質(zhì)膜完整率和頂體完整率顯著低于其他組(P0.05)。表明添加5 mmol/L的Tau或4 mmol/L的Gln對維持精子活率,保護精子質(zhì)膜,增強精子抗氧化能力效果顯著。3.在“稀釋液3”中分別添加不同濃度的五味子乙素(Sch B)和三七皂甙R1(Notoginsenoside R1),表明添加15μmol/L的Sch B精子活率、質(zhì)膜完整率、T-AOC濃度顯著高于其他組(P0.05),MDA濃度顯著低于其他組(P0.05),保存第5 d時,精子活率、質(zhì)膜完整率、T-AOC濃度、MDA濃度分別為0.61、50.71%、4.82 IU/mL、1.45 nmol/mL;添加1、5、10、15μmol/L的Sch B,對精子頂體完整率有明顯的保護作用,但組間差異不顯著(P0.05)。添加5 mmol/L的三七皂甙R1精子質(zhì)膜完整率、頂體完整率、T-AOC的濃度顯著高于其他組(P0.05),保存第5 d時,精子質(zhì)膜完整率為57.10%、頂體完整率為73.70%、T-AOC的濃度為4.65 IU/mL;添加1.25、2.5、5、10 mmol/L的三七皂甙R1對降低MDA濃度作用不顯著(P0.05);保存第1~3 d,添加1.25、2.5、5、10 mmol/L的三七皂甙R1對降低MDA濃度作用明顯,組間有差異性,但差異不顯著(P0.05)。因此,Sch B可顯著提高精液保存品質(zhì),提高豬精液常溫保存時抗氧化能力,其最適添加量為15μmol/L。三七皂甙R1可維持精子形態(tài)完整,提高豬精液常溫保存時抗氧化能力,其最適添加量為5 mmol/L。
[Abstract]:In order to study the effect of amino acid protectant (taurine, L- glutamine) and active ingredients of Chinese medicine (schisandrin B, 37 saponins R1) on the preservation of pig semen at room temperature, first of all 4 kinds of diluent formulations were designed to detect the sperm viability, effective preservation time, the integrity rate of plasma membrane, the integrity of the acrosome, and the best preservation effect. The basic formula was added to the basic formula with 0.5,1,5,10 mmol/L added taurine (Tau), L- glutamine (Gln) of 0.5,1,2,4,8,16 mmo/L, 1,5,10,15 u mol/L schisandin (Sch B) and 0.5,1,2,4,8,16 purified glutamine, and the white control group was set up to detect the quality of semen and the total antioxidant capacity. The effect of two aldehyde (MDA) on the preservation effect of pig semen was analyzed and the optimum addition was determined. The aim of this study was to develop a new type of diluted powder for the preservation of the new pig semen at normal temperature. The main results were as follows: 1. in the diluents of 4 kinds of self matched pig semen, the diluent was 3, the main components were glucose 40 g/L, sodium citrate 6.8 g/L, and sodium bicarbonate 0.8. G/L, EDTA1.5 g/L, 0.3 g/L citric acid and 0.7 g/L of potassium chloride, the effective preservation time is significantly higher than the other 3 diluents (P0.05), the effective preservation time is 4.4 D, the sperm viability of fourth D is 0.61, the integrity rate of the plasma membrane is 57.32%, the integrity rate of the acrosome is 78.12%, and the second is "diluent 2" and "diluent 4", and the worst preservation effect is "dilution". Liquid 1 ". Therefore," diluent 3 "is the best thinning solution for the preservation of 4 kinds of self matched pig semen at normal temperature, which can be used as the base diluent.2. to add different concentrations of taurine (Tau) and L- glutamine (Gln) in the basic diluent" diluent 3 ", indicating that the sperm viability and acrosome are intact when the 5 mmol/L Tau is added. The concentration of total antioxidant capacity (T-AOC) was significantly higher than that of other groups (P0.05), and the concentration of malondialdehyde (MDA) was significantly lower than that of other groups (P0.05). The sperm viability was 0.66, the acrosome integrity rate was 47.48%, the integrity rate of the plasma membrane was 47.48%, the membrane integrity rate was 72.10%, the concentration of T-AOC was 4.28 IU/mL, the concentration of MDA was 1.63 nmol/mL. adding 4 mmol/L Gln, the sperm viability, plasma membrane integrity rate and acrosome. The intact rate was significantly higher than that of the other groups (P0.05). The sperm viability was 0.59, the sperm membrane integrity rate was 47.05%, the integrity rate of the acrosome was 74.40%, the T-AOC concentration was 4.34 IU/mL, and the MDA concentration was 1.51 nmol/mL; the sperm viability, the intact rate of the plasma membrane and the integrity of the acrosome were significantly lower than those of the other groups (P0.05), indicating the Tau or 4 mmol/L to add 5 mmol/L. The effect of Gln on maintaining sperm viability, protecting the sperm plasma membrane and enhancing the antioxidant capacity of sperm was significant.3. in "diluent 3", adding different concentrations of schisandrin B (Sch B) and 37 saponins R1 (Notoginsenoside R1), indicating that the sperm viability of adding 15 u mol/L Sch B, the integrity of plasma membrane and T-AOC concentration were significantly higher than those of the other groups (P0.05). The concentration of the sperm was significantly lower than that of the other groups (P0.05). The sperm viability, plasma membrane integrity, T-AOC concentration, and MDA concentration were 0.61,50.71%, 4.82 IU/mL, 1.45 nmol/mL, respectively, when the preservation of fifth D, and Sch B with 1,5,10,15 micron mol/L had a significant protective effect on the sperm acrosome integrity, but there was no significant difference between the groups (P0.05). The sperm quality was added to the 37 saponins of 5. The membrane integrity, acrosome integrity, T-AOC concentration was significantly higher than that of other groups (P0.05). When the preservation of fifth D, the sperm plasma membrane integrity rate was 57.10%, the acrosome integrity rate was 73.70%, the T-AOC concentration was 4.65 IU/mL, and the 37 saponins R1 added to 1.25,2.5,5,10 mmol/L was not significant (P0.05) for reducing the MDA concentration (P0.05). 1~3 D, added 1~3 D, was added. The effect of 37 saponins R1 on reducing the concentration of MDA was obvious, but the difference was not significant, but the difference was not significant (P0.05). Therefore, Sch B could improve the preservation quality of semen and improve the antioxidant ability of the pig semen at room temperature. The optimum addition amount of 15 mu 37 saponins R1 can maintain the complete morphology of the sperm and improve the antioxidant activity of the pig semen at room temperature. Ability, the optimum amount is 5 mmol/L.

【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S828

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本文編號：1807398