本文選題：中山病 + 微量病毒中和試驗(yàn)�。� 參考：《中國(guó)預(yù)防獸醫(yī)學(xué)報(bào)》2017年01期

【摘要】：2014年本實(shí)驗(yàn)室在廣西分離獲得一株中山病病毒,表明中山病在我國(guó)已經(jīng)存在。為調(diào)查中山病在我國(guó)的流行分布狀況,本實(shí)驗(yàn)采用經(jīng)典的病毒微量中和試驗(yàn)方法,對(duì)我國(guó)黑龍江、內(nèi)蒙古、新疆、山東、江蘇、湖北、廣西、云南和海南等9個(gè)省區(qū)的543份牛羊血清進(jìn)行檢測(cè)。其中,海南68份牛羊血清樣品中陽(yáng)性樣品39份,陽(yáng)性率高達(dá)57.35%;黑龍江的80份樣品檢測(cè)結(jié)果均為陰性;其他各省的血清陽(yáng)性率介于6%~48.65%。結(jié)果表明,目前被調(diào)查的9個(gè)省區(qū)除黑龍江省外均存在中山病的流行,南方的感染率較高;對(duì)新疆、湖北、廣西、云南4個(gè)省自治區(qū)牛和羊的易感性進(jìn)行比較,除新疆外,牛的陽(yáng)性率均比羊高,牛血清陽(yáng)性率6%~58.5%,羊16.67%~33.33%;對(duì)不同季節(jié)中山病感染率進(jìn)行比較,結(jié)果顯示,秋季(9~11月)感染率(16.67%~43.33%)最高,明顯高于春季和夏季。由于采集的血清來(lái)自具有代表性的省份,以上研究結(jié)果表明,中山病在我國(guó)的流行具有廣泛性。
[Abstract]:A strain of Zhongshan disease virus was isolated in Guangxi in 2014, indicating that Zhongshan disease exists in China. In order to investigate the epidemic distribution of Zhongshan disease in China, the classical virus neutralization test method was used to study the prevalence of Zhongshan disease in Heilongjiang, Inner Mongolia, Xinjiang, Shandong, Jiangsu, Hubei, Guangxi, China. 543 serum samples of cattle and sheep from 9 provinces including Yunnan and Hainan were detected. Among them, 39 were positive in 68 cattle and sheep serum samples in Hainan, the positive rate was as high as 57.355,80 samples in Heilongjiang were negative, and the positive rates in other provinces were between 68.65 and 48.65. The results showed that the prevalence of Zhongshan disease existed in all the nine provinces and regions, except Heilongjiang Province, and the infection rate in the south was relatively high, and the susceptibility of cattle and sheep in Xinjiang, Hubei, Guangxi and Yunnan provinces was compared, except Xinjiang. The positive rate of cattle was higher than that of sheep. The positive rate of bovine serum was 58.5 and 16.677.The infection rate of Zhongshan disease in different seasons was higher than that of sheep. The results showed that the infection rate of Zhongshan disease in autumn was the highest, which was significantly higher than that in spring and summer. Since the serum samples were collected from representative provinces, the results show that the prevalence of Zhongshan disease in China is widespread.
【作者單位】： 黑龍江八一農(nóng)墾大學(xué)動(dòng)物科技學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所牛羊傳染病研究創(chuàng)新團(tuán)隊(duì);
【基金】：國(guó)家公益性(農(nóng)業(yè))科研專項(xiàng):重要牛羊蟲(chóng)媒病毒病防控關(guān)鍵技術(shù)研究與應(yīng)用(201303035)
【分類號(hào)】：S855.3

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