本文選題：丹翹灌注液 + 炎癥模型��； 參考：《甘肅農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：子宮內(nèi)膜炎是奶牛的常發(fā)病之一,多發(fā)于產(chǎn)后,是子宮內(nèi)膜感染引起的炎癥,常導(dǎo)致奶牛不孕。奶�；荚摬≈率巩a(chǎn)犢間期延長(zhǎng),受孕率、妊娠率降低,產(chǎn)奶量下降,治療費(fèi)用和淘汰率增加,這給奶牛養(yǎng)殖業(yè)造成了巨大的經(jīng)濟(jì)損失。目前多采用抗生素、激素等治療奶牛子宮內(nèi)膜炎,但隨著抗菌藥物、激素等的長(zhǎng)期大劑量應(yīng)用常造成奶中藥物殘留,食用后危及人類(lèi)健康。中藥有提高動(dòng)物機(jī)體免疫機(jī)能、抵抗疾病的能力,因其毒副作用小、殘留少、特異性強(qiáng)成為人們積極探索的方向。丹翹灌注液是根據(jù)中獸醫(yī)學(xué)理論和子宮內(nèi)膜炎的病理特點(diǎn)研制而成的用于防治奶牛子宮內(nèi)膜炎的中藥制劑,但丹翹灌注液對(duì)奶牛子宮內(nèi)膜炎是否具有抗炎作用,其抗炎作用機(jī)制如何?本研究從以下3個(gè)方面對(duì)丹翹灌注液的體內(nèi)、體外抗炎、鎮(zhèn)痛作用及其機(jī)制進(jìn)行了研究,以期為臨床試驗(yàn)用藥及進(jìn)一步研發(fā)抗炎中藥制劑提供理論基礎(chǔ)和依據(jù)。1、丹翹灌注液的體內(nèi)抗炎作用研究。采用二甲苯致耳廓腫脹實(shí)驗(yàn)、醋酸致毛細(xì)血管通透性增加實(shí)驗(yàn)、棉球致肉芽腫增生實(shí)驗(yàn)評(píng)價(jià)其體內(nèi)抗炎作用。結(jié)果顯示,40g/kg丹翹灌注液對(duì)二甲苯致耳廓腫脹有極顯著抑制作用、40g/kg、20g/kg丹翹灌注液對(duì)醋酸致毛細(xì)血管通透性增加、棉球致肉芽腫增生有顯著抑制作用,表明40g/kg以上的丹翹灌注液對(duì)急、慢性炎癥都有一定的抑制作用。2、丹翹灌注液的體內(nèi)鎮(zhèn)痛作用研究。采用醋酸誘導(dǎo)扭體反應(yīng)和福爾馬林致痛實(shí)驗(yàn)評(píng)價(jià)其鎮(zhèn)痛作用。結(jié)果顯示,40g/kg、20 g/kg丹翹灌注液對(duì)醋酸誘導(dǎo)的扭體反應(yīng)、福爾馬林致痛第Ⅱ相疼痛有顯著的抑制作用。表明丹翹灌注液的鎮(zhèn)痛活性主要參與外周炎癥鎮(zhèn)痛。3、丹翹灌注液的體外抗炎作用及抗炎機(jī)制研究。利用LPS刺激小鼠單核/巨噬細(xì)胞RAW 264.7建立體外炎癥模型,研究丹翹灌注液對(duì)炎癥模型細(xì)胞的炎性介質(zhì)和炎性細(xì)胞因子(NO、PGE2、TNF-α和IL-6)的影響,以及對(duì)NF-κB信號(hào)通路的影響。結(jié)果顯示丹翹灌注液(600μg/m L、300μg/m L、100μg/m L)作用于炎性細(xì)胞后能顯著抑制LPS誘導(dǎo)的NO的分泌,TNF-α、IL-6、i NOS和COX-2基因的表達(dá)及胞核內(nèi)NF-κB P65蛋白的表達(dá),且具有一定的劑量效應(yīng),只有600μg/m L的丹翹灌注液對(duì)PGE2、TNF-α和IL-6的分泌顯著抑制。結(jié)果表明丹翹灌注液對(duì)LPS誘導(dǎo)的炎癥反應(yīng)具有保護(hù)作用,其抗炎作用可能與調(diào)節(jié)NF-κB信號(hào)通路有關(guān),主要是抑制炎性過(guò)程中關(guān)鍵酶和炎癥因子的基因表達(dá)及炎癥介質(zhì)的產(chǎn)生。然而,丹翹灌注液作用炎性細(xì)胞后,炎性細(xì)胞因子IL-6及炎癥限速酶i NOS和COX-2基因的表達(dá)與正常對(duì)照組仍存在顯著差異,說(shuō)明丹翹灌注液有抗炎作用,但不能完全阻斷細(xì)胞炎癥反應(yīng)。
[Abstract]:Endometritis is one of the most common diseases in dairy cows. It is a kind of inflammation caused by endometrial infection and often leads to infertility. The disease causes prolonged calving interval, lower pregnancy rate, lower milk yield, higher treatment cost and higher elimination rate, which has caused enormous economic losses to the dairy cattle breeding industry. At present, antibiotics and hormones are used to treat endometritis in dairy cows. However, with the long-term use of antibiotics and hormones, the drug residues in milk are often caused, and human health is endangered after consumption. Traditional Chinese medicine has the ability to improve the immune function of animal body and resist disease. Because of its small toxicity, little residue and strong specificity, it has become an active exploration direction. Danqiao Perfusion liquid is a traditional Chinese medicine preparation for the prevention and treatment of endometritis in dairy cows, according to the theory of traditional Chinese veterinarian science and the pathological characteristics of endometritis. However, does Danqiao Perfusion liquid have anti-inflammatory effect on endometritis of dairy cows? What is the mechanism of its anti-inflammatory effect? In this study, the anti-inflammatory and analgesic effects and mechanism of Danqiao perfusion solution in vivo and in vitro were studied in the following three aspects. In order to provide theoretical basis and basis for clinical trial and further research and development of anti-inflammatory traditional Chinese medicine preparation. 1. In vivo anti-inflammatory effect of Danqiao perfusion solution. Auricular swelling induced by xylene, capillary permeability increase induced by acetic acid, granulomatous hyperplasia induced by cotton ball were used to evaluate its anti-inflammatory effect in vivo. The results showed that 40 g / kg Danqiao perfusion solution could significantly inhibit the auricle swelling induced by xylene. 40 g / kg danqiao perfusion solution could significantly inhibit the capillary permeability induced by acetic acid and the granuloma proliferation induced by cotton ball. It indicated that the perfusion solution above 40g/kg had an acute effect on auricle swelling. Chronic inflammation has a certain inhibitory effect. 2. Study on the analgesic effect of Danqiao Perfusion liquid in vivo. The analgesic effect was evaluated by acetic acid-induced writhing reaction and formalin induced pain test. The results showed that 40 g 路kg ~ (-1) 路kg ~ (-1) of 40 g 路kg ~ (-1) 路kg ~ (-1) 路min ~ (-1) g/kg could significantly inhibit acetic acid-induced writhing and formalin induced pain in phase 鈪，

本文編號(hào)：1791855