本文選題：魚腥草 + 質(zhì)量控制��； 參考：《西北農(nóng)林科技大學》2017年碩士論文

【摘要】：根據(jù)中獸醫(yī)學辨證論治的基本理論及中藥的功能主治,制備復方魚腥草顆粒劑,并進行體外抑菌試驗以及急性毒性試驗來確定其抑菌效果及安全性,為臨床的應用提供參考依據(jù)。1.單藥和復方的體外抑菌試驗采用二倍管稀釋法測定了魚腥草、黃芪、連翹、金銀花以及復方魚腥草對金黃色葡萄球菌、無乳鏈球菌、大腸埃希菌、沙門菌、多殺性巴氏桿菌的最小抑菌濃度(MIC)和最小殺菌濃度(MBC)。結(jié)果顯示,復方魚腥草的抑菌效果大于各單藥。2.復方魚腥草顆粒劑的制備與質(zhì)量控制試驗將魚腥草、黃芪、連翹和金銀花按42%、9%、4.5%和4.5%以及β-環(huán)糊精40%的比例,以手工濕法制備復方魚腥草顆粒劑。顆粒劑的粒徑介于200μm~2000μm之間,顏色為棕褐色�？疾鞆头紧~腥草顆粒劑的粒度、干燥失重、溶化性等指標,并用紫外分光光度含量分析方法測定了復方魚腥草顆粒劑中總黃酮和多糖的含量。結(jié)果顯示,復方魚腥草顆粒劑的粒度、干燥失重、溶化性等指標均符合獸藥典規(guī)定;總黃酮的平均含量為39.1 mg/g,多糖的平均含量為281.9 mg/g,分別為標示量的97.8%、94.0%,符合藥物制劑的要求。3.復方魚腥草顆粒劑的體外抑菌試驗采用二倍管稀釋法測定了復方魚腥草顆粒劑對金黃色葡萄球菌、無乳鏈球菌、大腸埃希菌、沙門菌、多殺性巴氏桿菌的最小抑菌濃度(MIC)和最小殺菌濃度(MBC),結(jié)果顯示,復方魚腥草顆粒劑對以上5種細菌的MIC(mg/mL)分別為45.71、45.71、182.86、365.71、182.86;MBC(mg/mL)分別為45.71、91.43、365.71、731.43、365.71。與復方魚腥草煎液的體外抑菌試驗結(jié)果比較可以發(fā)現(xiàn),將復方魚腥草煎液制成顆粒劑對抑菌和殺菌效果沒有影響。4.復方魚腥草顆粒劑的急性毒性試驗根據(jù)急性毒性試驗對復方魚腥草顆粒劑進行安全性評價,測得LD50大于10000mg/kg。根據(jù)WHO規(guī)定,如果LD50在5000~15000 mg/kg,為實際無毒藥物。故復方魚腥草顆粒劑安全性良好,屬于實際無毒藥物,臨床使用安全可靠。
[Abstract]:According to the basic theory of Chinese veterinary medicine and the function of traditional Chinese medicine, compound Houttuynia cordata granules were prepared, and the bacteriostatic test and acute toxicity test in vitro were carried out to determine the bacteriostatic effect and safety of compound Houttuynia cordata granules. To provide reference basis for clinical application. 1. In vitro bacteriostatic tests of single drug and compound were used to determine the effects of Houttuynia cordata, Astragalus membranaceus, Forsythia suspensa, Flos Lonicerae and compound Houttuynia on Staphylococcus aureus, Streptococcus alacinus, Escherichia coli and Salmonella. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Pasteurella multocida. The results showed that the bacteriostatic effect of compound Houttuynia cordata was higher than that of each single drug. Preparation and quality control test of compound Houttuynia granules compound Houttuynia granules were prepared by manual wet method with the ratio of 42Houttuynia, Astragalus membranaceus, Forsythia Forsythia and Flos Lonicerae at the ratio of 4.5% and 4.5% and 40% of 尾 -cyclodextrin. The particle size was between 200 渭 m and 2000 渭 m, and the color was brown. The granularity, dry weight loss and solubility of compound Houttuynia cordata granules were investigated. The contents of total flavonoids and polysaccharides in compound Houttuynia cordata granules were determined by UV spectrophotometry. The results showed that the granularity, dry weight loss and dissolution of compound Houttuynia granules were in accordance with the requirements of the Veterinary Pharmacopoeia, the average content of total flavonoids was 39.1 mg / g, and the average content of polysaccharides was 281.9 mg / g, respectively, which was 97.8% and 94.0% of the marked amount, which was in line with the requirements of drug preparation. In vitro bacteriostatic test of compound Houttuynia cordata granules the effects of compound Houttuynia granules on Staphylococcus aureus, Streptococcus lactococcus, Escherichia coli and Salmonella were determined by double tube dilution method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBCL) of Pasteurella multocida were respectively 45.71and 45.71182.86MBCmg / mL, respectively. The results showed that the MICM / mLs of compound Houttuynia cordata granules against the above five bacteria were 45.71and 365.1182.86MBCmg / mL, respectively, and 45.71C / mL91.43365.71,731.43365.71, respectively. Compared with compound decoction of Houttuynia houttuyniae in vitro, it was found that the preparation of compound decoction of Houttuynia cordata had no effect on bacteriostatic and bactericidal effect. Acute toxicity test of compound Houttuynia cordata granules according to acute toxicity test the safety of compound Houttuynia cordata granules was evaluated and the LD50 was measured to be more than 10 000 mg / kg. According to WHO, if LD50 is at 5, 000 g / kg, it is actually non-toxic. Therefore, compound Houttuynia granules are safe and safe in clinical use.
【學位授予單位】：西北農(nóng)林科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S853.7

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