本文選題：離子色譜 + 紫外檢測��； 參考：《色譜》2017年10期

【摘要】：通過對色譜柱、流動相洗脫、樣品前處理等條件進(jìn)行優(yōu)化,建立了一種檢測飼料中胍基乙酸含量的離子色譜法。在甲磺酸線性梯度洗脫條件下,樣品經(jīng)Dionex IonPacTMCS16陽離子交換柱分離,用紫外檢測器于200 nm波長處進(jìn)行檢測。在0.5~200 mg/L范圍內(nèi),中胍基乙酸色譜峰面積與質(zhì)量濃度呈良好的線性關(guān)系(相關(guān)系數(shù)r2=0.999 9)。配合飼料和濃縮飼料中胍基乙酸的檢出限為4.5 mg/kg、定量限為15 mg/kg,復(fù)合預(yù)混合飼料中胍基乙酸的檢出限為9.0 mg/kg、定量限為30 mg/kg。該方法對添加量在15 mg/kg~60 g/kg范圍內(nèi)的禽用配合飼料、豬用配合飼料、濃縮飼料、復(fù)合預(yù)混合飼料中胍基乙酸的回收率均大于94%。該方法性能指標(biāo)可滿足飼料中胍基乙酸含量的檢測需求。
[Abstract]:An ion chromatography method for the determination of guanidine acetic acid in feed was established by optimizing the chromatographic column, mobile phase elution and sample pretreatment. Under the condition of linear gradient elution of Mesonic acid, the samples were separated by Dionex IonPacTMCS16 cation exchange column and detected by UV detector at 200nm. In the range of 0.5 ~ 200 mg/L, there was a good linear relationship between the peak area and the mass concentration of guanidine acetic acid (r _ 2 ~ (2)) 0.999 ~ (9) 渭 m ~ (-1). The detection limit and quantification limit of guanidine acetic acid in formulated feed and concentrated feed were 4.5 mg / kg and 15 mg / kg respectively. The detection limit of guanidine acetic acid in compound premixed feed was 9.0 mg / kg and the quantitative limit was 30 mg / kg. The recoveries of guanidine acetic acid in fowl feed, pig feed, concentrated feed and compound premixed feed were all greater than 94% in the range of 15 mg/kg~60 g/kg. The performance index of this method can meet the requirement of the determination of guanidine acetic acid in feed.
【作者單位】： 中國農(nóng)業(yè)大學(xué)動物營養(yǎng)學(xué)國家重點實驗室農(nóng)業(yè)部飼料效價與安全監(jiān)督檢驗測試中心(北京);北京君德同創(chuàng)農(nóng)牧科技股份有限公司;
【基金】：北京市科技計劃項目(Z151100001215001)~~
【分類號】：O657.7;S816.17
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本文編號：1779430