本文選題：蓮藍(lán)口服液 + 制備工藝　； 參考：《河南科技大學(xué)》2015年碩士論文

【摘要】：目的:治療溫?zé)岵〕跗诘闹兴幏絼┹^多,其中中國獸藥典中收載的有“清解合劑”、“清溫?cái)《旧ⅰ�、“銀翹散”、“雙黃連口服液”等,這些方劑的藥材價(jià)格較昂貴、生產(chǎn)成本高,限制其在養(yǎng)殖生產(chǎn)中的使用。為選擇高效價(jià)廉的預(yù)防治療方劑,本試驗(yàn)選用板藍(lán)根、大青葉、穿心蓮三味中藥重新組方并對其提取工藝、藥效學(xué)、質(zhì)量控制、毒理學(xué)進(jìn)行研究,旨在研制對溫?zé)岵〕跗诘姆乐尉哂写_切效果、性價(jià)比高的中獸藥方劑。方法:(1)以R-S告依春、穿心蓮內(nèi)酯和脫水穿心蓮內(nèi)酯的含量測定為依據(jù),篩選各成分含量均較高的蓮藍(lán)口服液提取方法及其組方。(2)用制備實(shí)驗(yàn)中的三種口服液樣品對溫?zé)岵〕跗谛“资竽Ｐ瓦M(jìn)行預(yù)防及治療的藥效學(xué)實(shí)驗(yàn),以確定蓮藍(lán)口服液的最佳組方。(3)通過對穿心蓮、板藍(lán)根、大青葉進(jìn)行薄層鑒別,穿心蓮和板藍(lán)根的含量測定等方法對篩選出的方劑進(jìn)行質(zhì)量可控性研究。(4)對小鼠進(jìn)行急性毒性實(shí)驗(yàn),以及對大鼠進(jìn)行連續(xù)給藥30天的長期毒性試驗(yàn),來進(jìn)行蓮藍(lán)口服液的安全性評價(jià)。結(jié)果:(1)蓮藍(lán)口服液的制備實(shí)驗(yàn)中,板藍(lán)根和大青葉提取液用水提醇沉法獲得,穿心蓮用75%乙醇溫浸提取,板藍(lán)根、大青葉與穿心蓮提取液按原藥材2:1:1的比例混合后制得的蓮藍(lán)口服液各標(biāo)示成分含量較高。(2)藥效學(xué)實(shí)驗(yàn)中,板藍(lán)根、大青葉與穿心蓮提取液按原藥材2:1:1的比例混合后制得的蓮藍(lán)口服液的解熱效果最好。(3)分別建立了蓮藍(lán)口服液中大青葉、板藍(lán)根和穿心蓮的薄層鑒別方法和大青葉、穿心蓮的含量測定方法。質(zhì)量研究表明,方法均無陰性干擾,可以用來控制蓮藍(lán)口服液的質(zhì)量。(4)急性毒性試驗(yàn)中小鼠給藥量達(dá)40000 mg/kg體重,7天內(nèi)所有小鼠沒有死亡,各組剖解檢查亦無明顯改變;長期毒性試驗(yàn)中各給藥組動(dòng)物的體重增重、血常規(guī)、血液生化學(xué)指標(biāo)與對照組相比均無顯著性差異,各組剖檢及組織病理學(xué)檢查亦無明顯改變。說明蓮藍(lán)口服液沒有明顯的毒性作用。結(jié)論:蓮藍(lán)口服液的制備工藝簡單快速,藥效顯著,建立的檢測方法可行、穩(wěn)定,可有效地控制蓮藍(lán)口服液的質(zhì)量。此組方制備的蓮藍(lán)口服液未見明顯毒性,可進(jìn)一步開發(fā)為新獸藥。
[Abstract]:Objective: there are many traditional Chinese medicine prescriptions for the treatment of warm fever. Among them, the Chinese veterinary pharmacopoeia contains "Qingjie mixture", "Qingwen Baidu Powder", "Yinqiao Powder", "Shuanghuanglian Oral liquid" and so on.High production cost limits its use in aquaculture production.In order to select effective and inexpensive preventive and therapeutic prescriptions, we selected Radix Isatidis, Radix Isatidis and andrographis andrographis, and studied their extraction technology, pharmacodynamics, quality control and toxicology.The purpose of this study was to develop a Chinese veterinary medicine prescription with high performance and cost-effective in the early stage of prevention and treatment of warm fever.Methods the contents of andrographolide and dehydrated andrographolide were determined on the basis of R-S Gouyichun.To select the extraction method of Lianlan oral liquid with high content of each component and its formula. 2) to use the three kinds of oral liquid samples in the preparation experiment to carry on the pharmacodynamics experiment to prevent and treat the mice model of febrile fever in the early stage.In order to determine the best prescription of Lianlan oral liquid, through thin layer identification of andrographis andrographis, Radix Isatidis and Radix Isatidis,The contents of andrographis andrographis and Radix Isatidis were determined by the methods of quality controllability of the selected prescription. The acute toxicity test was carried out in mice and the long-term toxicity test was carried out in rats for 30 days.To evaluate the safety of Lianlan oral liquid.Results in the preparation experiment of Lianlan oral liquid, Isatis Isatidis and Radix Isatidis were obtained by water extraction and alcohol precipitation, andrographis andrographis was extracted by 75% ethanol, and Radix Isatidis was extracted.Radix Isatidis and andrographis andrographis extract were mixed at 2:1:1 of the original medicinal materials, and the contents of each marked components of Lianlan oral liquid were higher than that of Radix andrographis) in the pharmacodynamics experiment, Isatidis root, Radix Isatidis, Radix Isatidis,The best antipyretic effect of Lianlan oral liquid was obtained by mixing the extract of Daqingye and andrographis andrographis according to the proportion of the original medicine at 2:1:1). The thin layer identification method of Daqingye, Isatidis root and andrographis andrographis in Lianlan oral liquid was established respectively.Determination of andrographis paniculata content.The quality study showed that all the methods had no negative interference and could be used to control the quality of Lianlan oral liquid.There was no significant difference in weight gain blood routine blood biochemical indexes and histopathological examination between each group and the control group in long-term toxicity test.It indicated that Lianlan oral liquid had no obvious toxic effect.Conclusion: the preparation process of Lianlan oral liquid is simple and rapid, and its pharmacodynamics is remarkable. The established method is feasible and stable, and can effectively control the quality of Lian Lan oral liquid.Lianlan oral liquid prepared by this prescription has no obvious toxicity and can be further developed as a new veterinary drug.
【學(xué)位授予單位】：河南科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S853.7

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