本文選題：復(fù)合抗菌肽“態(tài)康利保” + 仔豬�。� 參考：《四川農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：試驗(yàn)研究了不同劑量的復(fù)合抗菌肽制劑“態(tài)康利�！睂�(duì)斷奶仔豬體液免疫及細(xì)胞因子水平的影響,并探索“態(tài)康利保”的最佳添加量及其作用機(jī)理,力求找到一種綠色、高效的天然免疫增強(qiáng)劑。試驗(yàn)選取17日齡接種豬瘟兔化弱毒疫苗、21日齡斷奶的臨床健康且豬瘟抗體水平相近(25日齡檢測(cè)豬瘟抗體水平)的仔豬90頭,隨機(jī)分為5組。試驗(yàn)期為26-53日齡,Ⅰ組飼喂基礎(chǔ)日糧,Ⅱ組在飼料中添加黃芪多糖400mg/kg, Ⅲ、 Ⅳ、Ⅴ組在飼料中分別添加“態(tài)康利保'250mg/kg、500mg/kg、1000mg/kg。分別在32日齡、39日齡、46日齡、53日齡每組隨機(jī)選取6頭仔豬前腔靜脈采血,分離血清后分別用于豬瘟抗體水平、免疫球蛋白(IgG、IgM、IgA)、總補(bǔ)體(ch50)及細(xì)胞因子(IL-2、IL-4、IL-6、IFN-γ、TNF-α)含量的測(cè)定。試驗(yàn)結(jié)果：(1)“態(tài)康利保”和黃芪多糖均能提高豬瘟抗體水平,且“態(tài)康利�！本S持抗體水平更為持久,以“態(tài)康利保”1000mg/kg添加量效果最為明顯。(2)“態(tài)康利保'1000mg/kg飼料組可明顯提高仔豬血清中IgG、IgA及IgM的含量(P0.05或P0.01),且以連續(xù)飼喂3周效果最為明顯。“態(tài)康利�！�500mg/kg飼料組與黃芪多糖組均可提高血清IgG含量,但兩組差異不明顯；“態(tài)康利保"500mg/kg飼料組可有效提高血清IgM水平；“態(tài)康利保’'1000mg/kg組能較早提高血清Iga含量。 (3)“態(tài)康利保”和黃芪多糖均能極顯著的提高血清ch50含量(P0.01),且“態(tài)康利�！苯M優(yōu)于黃芪多糖組,以“態(tài)康利�！�1000mg/kg添加量最為明顯。 (4)“態(tài)康利保”可提高血清中Th 1型IL-2、IFN-γ、TNF-α及Th2型IL-4、IL-6含量�！皯B(tài)康利保”1000mg/kg組與對(duì)照組相比,可極顯著提高血清IL-2、IL-6、IFN-γ和TNF-α含量(P0.01),從39日齡起,“態(tài)康利�！�1000mg/kg飼料組可顯著提高血清IL-4水平(P0.01)；黃芪多糖可一定程度提高血清IL-2、IL-4、IL-6、IFN-γ和TNF-α的含量,但效果不及“態(tài)康利�！薄＝Y(jié)論：復(fù)合抗菌肽“態(tài)康利�！笨擅黠@提高斷奶仔豬豬瘟抗體水平、免疫球蛋白、總補(bǔ)體ch50、細(xì)胞因子的含量,從而提高仔豬的免疫力。以日糧中添加1000mg/kg劑量連續(xù)飼喂3周以上效果最佳。
[Abstract]:The effects of different doses of compound antimicrobial peptide preparation "State Kanglibao" on humoral immunity and cytokine levels in weaned piglets were studied, and the optimum dosage and mechanism of "state Kanglibao" were explored to find a green.A highly effective innate immune enhancer.A total of 90 piglets were randomly divided into 5 groups, which were randomly divided into 5 groups, which were randomly divided into 5 groups, which were randomly divided into 5 groups, and 90 piglets inoculated with the attenuated hog fever vaccine at the age of 17 days and weaned at 21 days of age with similar levels of antibody to swine fever were randomly divided into 5 groups.The trial period was 26 to 53 days old. Group I was fed basic diet, group 鈪，

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