復(fù)合特異性卵黃抗體對(duì)18~28日齡犢牛生長(zhǎng)、腹瀉和免疫力的影響
發(fā)布時(shí)間:2018-04-17 13:42
本文選題:犢牛 + 卵黃抗體 ; 參考:《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2016年07期
【摘要】:本文旨在飼糧中添加復(fù)合特異性卵黃抗體(Ig Y)探究其對(duì)18~28日齡犢牛生長(zhǎng)、腹瀉和免疫力的影響。選取初生中國(guó)荷斯坦公犢牛20頭,10日齡之前飼喂鮮奶,之后隨機(jī)分為2組,對(duì)照(CON)組飼喂基礎(chǔ)飼糧;試驗(yàn)(IGY)組在基礎(chǔ)飼糧中日添加45 mg/kg BW的復(fù)合特異性Ig Y。預(yù)試期為犢牛11~17日齡,期間進(jìn)行代乳品過渡,正試期為18~28日齡。分別于犢牛18和28日齡晨飼前稱重,每天記錄犢牛采食量并進(jìn)行糞便評(píng)分。試驗(yàn)結(jié)束當(dāng)天(28日齡)晨飼前頸靜脈采血,測(cè)定血清中總抗氧化能力(T-AOC)和堿性磷酸酶(AKP)活性以及免疫球蛋白、一氧化氮(NO)、溶菌酶(LZM)濃度。結(jié)果表明:飼糧添加復(fù)合特異性Ig Y雖然對(duì)犢牛平均日增重和料重比無顯著影響(P0.05),卻有助于降低犢牛的糞便評(píng)分和腹瀉率,作用顯著(P0.05);IGY組較CON組極顯著提高了犢牛血清中免疫球蛋白G(Ig G)、免疫球蛋白M(Ig M)和免疫球蛋白A(Ig A)的濃度(P0.01),分別提高7.19%、5.71%和8.50%;IGY組T-AOC極顯著高于CON組(P0.01),而NO濃度卻顯著低于CON組(P0.05);IGY組血清LZM濃度極顯著低于CON組(P0.01),復(fù)合特異性Ig Y對(duì)血清AKP活性卻無顯著影響(P0.05)。結(jié)果提示,飼糧中添加復(fù)合特異性Ig Y通過提高犢牛免疫球蛋白濃度和抗氧化能力以及發(fā)揮Ig Y本身抗原抗體反應(yīng),增強(qiáng)犢牛免疫能力和抵抗外界應(yīng)激能力,降低了腹瀉率,促進(jìn)了犢牛健康。
[Abstract]:The aim of this study was to investigate the effects of compound specific yolk antibody Ig Y on growth, diarrhea and immunity of 1828 day-old calves.20 male Holstein calves were fed fresh milk before 10 days of age, then were randomly divided into two groups, the control group was fed with basic diet, and the trial group was fed with 45 mg/kg BW compound specific Ig Y.The pre-test period was 1117 days of calf, and the transition of milk substitute was carried out during the period of 18 ~ 28 days of age.The calves were weighed before morning feeding at the age of 18 and 28 days respectively. Daily intake of calves was recorded and faecal scores were scored.Blood samples were collected from the jugular vein before the morning feeding. The activities of T-AOC) and alkaline phosphatase (AKP) and the concentrations of immunoglobulin (Ig), nitric oxide (no) and lysozyme (LZM) in serum were measured.The results showed that the supplementation of compound specific Ig Y diet had no significant effect on the average daily gain and feed weight ratio of calves, but it could reduce the fecal score and diarrhea rate of calves.Compared with the CON group, the serum levels of immunoglobulin G(Ig Gluten (M(Ig M) and immunoglobulin A (Ig A) in the P0.05 + IGY group were significantly higher than those in the CON group (P 0.01), and the T-AOC level in the IGY group was significantly higher than that in the CON group (P 0.01), and the no concentration was significantly higher in the IGY group than in the CON group.The concentration of serum LZM in CON group was significantly lower than that in CON group (P 0.01), but complex specific IgY had no significant effect on serum AKP activity.The results suggested that the supplementation of compound specific IGY in diet could increase the immunoglobulin concentration and antioxidation ability of calves and play the role of IgY antigen-antibody reaction, enhance the immune ability of calves and resist external stress, and decrease the diarrhea rate.Improved calf health.
【作者單位】: 中國(guó)農(nóng)業(yè)科學(xué)院飼料研究所奶牛營(yíng)養(yǎng)學(xué)北京市重點(diǎn)實(shí)驗(yàn)室農(nóng)業(yè)部飼料生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】:奶牛產(chǎn)業(yè)技術(shù)體系北京市創(chuàng)新團(tuán)隊(duì) 中國(guó)農(nóng)科院科技創(chuàng)新工程 國(guó)家科技支撐計(jì)劃課題“華北農(nóng)區(qū)及北方大城市奶牛健康養(yǎng)殖生產(chǎn)技術(shù)集成及產(chǎn)業(yè)化示范”(2012BAD12B06)
【分類號(hào)】:S823.5
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