發(fā)布時(shí)間：2018-04-15 20:08

  本文選題：�；撬� + 半胱氨酸雙加氧酶��； 參考：《沈陽農(nóng)業(yè)大學(xué)》2016年碩士論文

【摘要】：牛磺酸是哺乳動(dòng)物體內(nèi)含量最為豐富的游離氨基酸之一,生理學(xué)功能十分廣泛,對(duì)生物機(jī)體器官及組織功能的發(fā)揮起著重要作用。哺乳動(dòng)物體內(nèi)牛磺酸的來源除自身的內(nèi)源性合成外,還可通過食物攝取來獲得。牛磺酸合成的關(guān)鍵酶有半胱氨酸雙加氧酶(CDO)和半胱亞磺酸脫羧酶(CSAD/CSD), CDO-CSD途徑合成牛磺酸是體內(nèi)�；撬嵘锖铣傻闹匾緩�,細(xì)胞需通過牛磺酸轉(zhuǎn)運(yùn)體(TAUT)對(duì)�；撬徇M(jìn)行轉(zhuǎn)運(yùn),以維持胞內(nèi)牛磺酸的濃度。目前關(guān)于牛磺酸在豬體內(nèi)的合成及其對(duì)豬的作用尚缺乏相關(guān)資料,本文通過RT-PCR、Western-blot和酶聯(lián)免疫吸附等方法檢測了牛磺酸生物合成關(guān)鍵酶-CDO、 CSD及轉(zhuǎn)運(yùn)體TAUT在豬肝臟、下丘腦、垂體、甲狀腺、腎上腺、睪丸和卵巢組織中的表達(dá)以及�；撬岬暮�,探討了這些組織中牛磺酸的來源,以期為實(shí)驗(yàn)室今后進(jìn)一步研究�；撬釋�(duì)豬的作用及其在豬生產(chǎn)中的應(yīng)用奠定前期基礎(chǔ)。試驗(yàn)結(jié)果證明：①長白豬肝臟組織CDO cDNA編碼區(qū)序列為609 bp,編碼區(qū)在1-603bp,編碼201個(gè)氨基酸；與GenBank中的野豬(Sus scrofa) CDO cDNA核苷酸序列同源性為99%,編碼的氨基酸序列同源性為100%。②所檢測的長白豬7個(gè)組織中均存在CDO、CSD、TAUTmRNA和蛋白的表達(dá),其中CDO mRNA表達(dá)由高到低的順序?yàn)椋翰G丸、卵巢、甲狀腺、肝臟、垂體、下丘腦、腎上腺；CSD mRNA表達(dá)由高到低的順序?yàn)椋杭谞钕佟⒋贵w、肝臟、卵巢、下丘腦、腎上腺、睪丸；TAUT mRNA表達(dá)由高到低的順序?yàn)椋捍贵w、肝臟、卵巢、下丘腦、甲狀腺、腎上腺、睪丸。③所檢測的長白豬血清和7個(gè)組織中均存在牛磺酸,且其含量由高到低為：垂體、下丘腦、腎上腺、肝臟、睪丸、卵巢、甲狀腺、血清。試驗(yàn)結(jié)果表明：�；撬釓V泛存在于豬垂體、下丘腦、腎上腺、肝臟、睪丸、卵巢和甲狀腺組織中,其來源除組織自身通過CDO-CSD途徑生物合成外,還需通過TAUT從血液中攝取。牛磺酸對(duì)垂體、下丘腦、腎上腺、肝臟、睪丸、卵巢和甲狀腺功能的發(fā)揮有一定的作用,但其確切作用尚需進(jìn)一步研究。
[Abstract]:Taurine is one of the most abundant amino acids in mammals, the physiological function is very extensive, play an important role on biological body organs and tissue function. In addition to their own source of endogenous synthesis of taurine in mammals, can also be obtained through food intake. The key enzyme of taurine synthesis with double cysteine oxygenase the enzyme (CDO) and cysteine sulfinate decarboxylase (CSAD/CSD), CDO-CSD pathway is an important way in the synthesis of taurine taurine biosynthesis, cell by taurine transporter (TAUT) transport of taurine, to maintain the intracellular concentration of taurine. There is still a lack of relevant data in the synthesis of taurine and its effect on pig pigs in this paper, through RT-PCR, Western-blot and ELISA assay taurine biosynthesis key enzyme -CDO, CSD and TAUT in transporter Pig liver, hypothalamus, pituitary, thyroid, adrenal, testis and ovarian tissue expression and taurine content, discusses the source of taurine in these tissues, in order to further study the effect of taurine on laboratory pig and its application in pig production to lay the preliminary foundation. Test results show that the liver cells of Landrace CDO cDNA encoding sequence was 609 BP, encoding in 1-603bp, encoding 201 amino acids; and in the GenBank (Sus scrofa) CDO wild boar cDNA nucleotide sequence homology was 99%, encoding the amino acid sequences of CDO, there were the 100%. detection for the 7 Landrace tissues CSD, TAUTmRNA and protein expression the expression of CDO, mRNA in the order from high to low: testis, ovary, liver, thyroid, pituitary, hypothalamus and adrenal gland; CSD mRNA expression from high to low in the order: thyroid, pituitary, liver The hypothalamus, adrenal, ovary, testis, TAUT; expression of mRNA from high to low order: pituitary, hypothalamus, ovary, liver, thyroid, adrenal, testis. There were 3 taurine detection in serum and 7 Landrace tissues, and its content from high to low: the hypothalamus, pituitary, adrenal gland. The liver, testis, ovary, thyroid gland, serum. The results showed that taurine exists in porcine pituitary, hypothalamus, adrenal gland, liver, testis, ovary and thyroid tissue, the sources of the organization itself through the pathway of CDO-CSD biosynthesis, also need uptake from the blood by TAUT. Taurine on hypothalamus, pituitary, adrenal gland, liver, testis, ovary and thyroid function has certain effect, but its exact role needs further study.

【學(xué)位授予單位】：沈陽農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S828

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