發(fā)布時間：2018-04-09 20:30

  本文選題：肉雞雞胚　切入點：脂滴　出處：《中國畜牧獸醫(yī)》2017年03期

【摘要】：為了探索雞胚肝臟脂滴的發(fā)育規(guī)律,選取120枚受精蛋,分別在孵化的第7~21天(E7~E21)每天隨機選取6枚蛋,收集雞胚,采集雞胚肝臟,采用冰凍切片、蘇丹Ⅲ染色觀察雞胚肝臟脂滴的形成和發(fā)育特點。結果發(fā)現(xiàn):1雞胚發(fā)育到E9胚齡,肝細胞內開始出現(xiàn)脂滴,且隨著胚齡增加,脂滴的數(shù)量不斷增多,脂滴的大小不斷增大;2E18胚齡以前,肝臟脂滴的分布從中央靜脈開始沿肝索到肝小葉邊緣數(shù)量逐漸減少,E18胚齡以后,肝臟邊緣脂滴數(shù)量較肝臟中央的多;3肝臟脂滴的發(fā)育分為E9~E14、E15~E17和E18~E21 3個階段,而且后一個階段脂滴的數(shù)量和體積均顯著大于前一個階段(P0.05),脂滴的平均大小在E9~E14、E15~E17和E18~E21 3個階段分別為3.2、5.7和10.8μm,平均數(shù)量分別為67、327、397個/mm2。以上結果表明,隨著雞胚的發(fā)育,肝細胞內脂滴數(shù)量增加、體積變大。
[Abstract]:In order to explore the development rule of lipid droplets in the liver of chicken embryo, 120 fertilized eggs were selected, 6 eggs were randomly selected every day on the 7th 21st day of hatching. Chicken embryo was collected, liver of chicken embryo was collected and frozen section was used.The formation and development of lipid droplets in the liver of chicken embryo were observed by Sudan 鈪，

本文編號：1728028