發(fā)布時(shí)間：2018-04-05 02:21

  本文選題：綠原酸　切入點(diǎn)：金黃色葡萄球菌　出處：《揚(yáng)州大學(xué)》2017年碩士論文

【摘要】：本文主要研究了綠原酸對(duì)金黃色葡萄球菌感染的乳腺上皮細(xì)胞的保護(hù)作用及綠原酸對(duì)奶牛中性粒細(xì)胞功能的影響。試驗(yàn)一、綠原酸對(duì)金黃色葡萄球菌與奶牛乳腺上皮細(xì)胞相互作用的影響研究0、10、20、30 μg/mL綠原酸對(duì)金黃色葡萄球菌增殖的抑制作用。結(jié)果顯示培養(yǎng)0、3、6 h時(shí)綠原酸對(duì)金黃色葡萄球菌的增殖沒有顯著影響,培養(yǎng)9、12 h時(shí)30 μg/mL綠原酸組對(duì)金黃色葡萄球菌增殖具有顯著的抑制作用(P0.05)。培養(yǎng)24h時(shí)10 μg/mL綠原酸組對(duì)金黃色葡萄球菌增殖具有顯著的抑制作用(P0.05),20、30μg/mL綠原酸組對(duì)金黃色葡萄球菌增殖具有極顯著的抑制作用(P0.01)。20μg/mL綠原酸可以對(duì)已形成的細(xì)菌生物膜起到破壞作用(P0.05),其他組無顯著影響。10、20、30μg/mL綠原酸對(duì)乳腺上皮細(xì)胞活性無負(fù)面影響,20 μg/mL綠原酸組可以顯著提高金黃色葡萄球菌感染的乳腺上皮細(xì)胞的存活率(P0.05),其他組無顯著影響。10、20、30 μg/mL綠原酸對(duì)金黃色葡萄球菌粘附侵襲乳腺上皮細(xì)胞沒有顯著影響,但30 μg/mL綠原酸組可以顯著降低金黃色葡萄球菌對(duì)乳腺上皮細(xì)胞的侵襲率(P0.05)。30 μg/mL綠原酸組可以顯著降低金黃色葡萄球菌誘導(dǎo)的乳腺上皮細(xì)胞ROS的產(chǎn)生(P0.05),其他組無顯著影響。試驗(yàn)二、綠原酸對(duì)金黃色葡萄球菌誘導(dǎo)的奶牛乳腺上皮細(xì)胞炎癥反應(yīng)的影響為探究綠原酸對(duì)乳腺上皮細(xì)胞炎癥相關(guān)因子表達(dá)的影響,利用金黃色葡萄球菌感染乳腺上皮細(xì)胞后加入綠原酸進(jìn)行培養(yǎng),結(jié)果顯示30 μg/mL綠原酸可以極顯著降低IL-1β、IL-6、IL-8、TLR-2mRNA 的表達(dá)(P0.01),對(duì) TNF-α、MyD88mRNA 的表達(dá)無顯著影響。30 μg/mL綠原酸組可以顯著降低MAPK信號(hào)通路p38、JNK蛋白的磷酸化水平,對(duì)ERK亞族的磷酸化沒有影響。試驗(yàn)三、綠原酸對(duì)中性粒細(xì)胞功能及炎癥相關(guān)基因表達(dá)的影響為探究綠原酸對(duì)中性粒細(xì)胞功能的影響,對(duì)中性粒細(xì)胞的趨化、吞噬、殺菌功能進(jìn)行評(píng)價(jià)。分別選用0、10、20、30 μg/mL綠原酸與中性粒細(xì)胞共培養(yǎng),結(jié)果顯示10、20、30 μg/mL綠原酸對(duì)中性粒細(xì)胞活性沒有影響,10、20、30 μg/mL綠原酸對(duì)中性粒細(xì)胞的趨化能力沒有影響,20μg/mL綠原酸組可以顯著提高中性粒細(xì)胞的吞噬能力(P0.05),30μg/mL組可以極顯著提高吞噬能力(P0.01)。添加20μg/mL綠原酸組中性粒細(xì)胞的殺菌能力顯著提高(P0.05),其他組無顯著影響。為探究綠原酸對(duì)中性粒細(xì)胞炎癥相關(guān)因子表達(dá)的影響,對(duì)趨化實(shí)驗(yàn)下室的中性粒細(xì)胞基因表達(dá)進(jìn)行分析,結(jié)果顯示,30μg/mL綠原酸組可以降低中性粒細(xì)胞IL-1β、TLR-2mRNA的表達(dá)(P0.05),但對(duì)TGF-β、IL-8 mRNA的表達(dá)無顯著影響。
[Abstract]:The protective effect of Lv Yuan acid on breast epithelial cells infected with Staphylococcus aureus and the effect of Lv Yuan acid on the function of neutrophils in dairy cattle were studied.Experiment 1. The effect of Lv Yuan acid on the interaction between Staphylococcus aureus and mammary epithelial cells of dairy cows was studied. The inhibitory effect of 0 ~ (10) ~ (20) ~ (20) 渭 g/mL Lv Yuan acid on the proliferation of Staphylococcus aureus was studied.The results showed that Lv Yuan acid had no significant effect on the proliferation of Staphylococcus aureus at 0 ~ 3h for 6 h, and the 30 渭 g/mL Lv Yuan acid group had a significant inhibitory effect on the proliferation of Staphylococcus aureus at 9 ~ 12 h.Inhibitory effect of 10 渭 g/mL Lv Yuan acid group on Staphylococcus aureus proliferation at 24 h. The inhibitory effect of 10 渭 g/mL Lv Yuan acid group on Staphylococcus aureus proliferation was significant. P0.01- .20 渭 g/mL Lv Yuan acid could inhibit the proliferation of Staphylococcus aureus biofilm.There was no significant effect on the activity of mammary epithelial cells in the other group. There was no negative effect on the activity of mammary epithelial cells in the other groups. The survival rate of mammary epithelial cells infected with Staphylococcus aureus was significantly increased in the 20 渭 g/mL Lv Yuan group, and no significant difference was found in the other groups, while in the other groups, the survival rate of breast epithelial cells infected with Staphylococcus aureus was significantly increased.The effect of #number0# 渭 g/mL Lv Yuan on Staphylococcus aureus adhesion and invasion of breast epithelial cells was not significant.However, 30 渭 g/mL Lv Yuan acid group could significantly reduce the invasion rate of Staphylococcus aureus on breast epithelial cells. 30 渭 g/mL Lv Yuan acid group could significantly reduce the ROS production induced by Staphylococcus aureus, but the other groups had no significant effect.In experiment 2, the effect of Lv Yuan acid on the inflammatory response of bovine mammary epithelial cells induced by Staphylococcus aureus was to explore the effect of Lv Yuan acid on the expression of inflammatory related factors in mammary epithelial cells.Staphylococcus aureus was infected with mammary epithelial cells and cultured with Lv Yuan acid.緇撴灉鏄劇ず30 渭g/mL緇垮師閰稿彲浠ユ瀬鏄捐憲闄嶄綆IL-1尾,IL-6,IL-8,TLR-2mRNA 鐨勮〃杈，

本文編號(hào)：1712767