本文選題：肺炎克雷伯菌　切入點(diǎn)：整合子　出處：《中國(guó)獸醫(yī)科學(xué)》2017年12期

【摘要】：通過(guò)菌落形態(tài)觀察、生化試驗(yàn)及16S rDNA基因序列測(cè)定對(duì)分離菌株進(jìn)行鑒定,共獲得7株豬源肺炎克雷伯菌,將其命名為Kp1~Kp7。致病性試驗(yàn)結(jié)果表明,7株分離菌株對(duì)小鼠均具有較強(qiáng)的致病性。藥敏試驗(yàn)結(jié)果顯示,分離菌株的耐藥表型差異性較大,對(duì)強(qiáng)力霉素、大觀霉素及阿米卡星的耐藥性較強(qiáng)。對(duì)分離菌株整合子的檢測(cè)表明,僅能從Kp1檢測(cè)到消毒劑-磺胺基因(qac EΔ1-sul1)、整合酶基因(IntⅠ)以及基因盒。本研究為肺炎克雷伯菌引起的疾病選擇合理的抗菌藥物提供了一定的科學(xué)依據(jù),并為進(jìn)一步研究其耐藥機(jī)制奠定了基礎(chǔ)。
[Abstract]:Seven strains of Klebsiella pneumoniae were identified by colony morphology, biochemical test and 16s rDNA gene sequencing.The results of pathogenicity test showed that all of the 7 isolated strains had strong pathogenicity to mice.The results of drug sensitivity test showed that the resistant phenotypes of the isolates were different, and the resistance to doxycycline, spectinomycin and amikacin was stronger.The detection of integron in isolated strains showed that only disinfectant sulfanilamide gene (QAC E 螖 1-sul1), integrase gene (Int 鈪，

本文編號(hào)：1711318