發(fā)布時(shí)間：2018-04-04 03:08

  本文選題：氣候變化　切入點(diǎn)：青藏高原　出處：《中國(guó)礦業(yè)大學(xué)》2017年碩士論文

【摘要】：氣候變暖逐漸成為國(guó)際研究熱點(diǎn)。青藏高原屬典型生態(tài)脆弱帶,該地區(qū)升溫幅度更加明顯并且降水發(fā)生改變,全球變暖已導(dǎo)致青藏高原大量冰川融化,進(jìn)而使該地區(qū)水循環(huán)和土壤水分發(fā)生巨大變化。基于青藏高原那曲地區(qū)不同梯度增溫和增水實(shí)驗(yàn)平臺(tái),利用定量PCR(qPCR)、末端限制性片段多態(tài)性(T-RFLP)方法,研究了1、3、13個(gè)月的增溫(1、2、4℃)和降水變化(增加降水100%、減少降水50%)對(duì)青藏高原高寒草甸土壤nir S、nirK、nosZ反硝化菌豐度、群落結(jié)構(gòu)和多樣性的影響。研究結(jié)果表明:該地區(qū)nirS和nosZ基因拷貝數(shù)約為nirK的10倍;增溫、降水變化以及增溫同時(shí)增加降水處理對(duì)nirK、nir S、nosZ反硝化菌豐度均沒(méi)有明顯影響;不同采樣時(shí)間對(duì)nosZ基因豐度的影響顯著,但對(duì)nirK和nir S基因豐度沒(méi)有顯著影響。nirS和nosZ反硝化菌群落結(jié)構(gòu)對(duì)增溫和降水變化的響應(yīng)比nirK反硝化菌敏感,nirS和nosZ反硝化菌在增溫或增減水處理1個(gè)月時(shí),群落結(jié)構(gòu)即發(fā)生了較明顯的變異,但nirK反硝化菌群落結(jié)構(gòu)在處理1個(gè)月時(shí),群落結(jié)構(gòu)變化不明顯,隨處理時(shí)間增加,群落結(jié)構(gòu)逐漸發(fā)生改變;增溫和降水變化之間對(duì)nirK、nirS、nosZ反硝化菌群落結(jié)構(gòu)的影響有一定的調(diào)控作用;相比增溫2℃和增加降水100%處理,不同采樣時(shí)間對(duì)nirK、nirS、nosZ反硝化菌群落結(jié)構(gòu)的影響占主導(dǎo)作用,并受到增溫和降水變化的影響;與增溫2℃處理相比,增加降水100%對(duì)nirK和nirS群落結(jié)構(gòu)對(duì)采樣時(shí)間效應(yīng)的影響更大,增加降水100%和增溫2℃均明顯影響nosZ群落結(jié)構(gòu)的采樣時(shí)間效應(yīng)。增溫和降水變化對(duì)nirK、nirS、nosZ反硝化菌群落多樣性沒(méi)有明顯的影響,不同采樣時(shí)間對(duì)多樣性也沒(méi)有明顯的影響。
[Abstract]:Climate warming has become a hot topic of international research. The Qinghai Tibet Plateau is a typical ecological fragile zone, the area of temperature rise and precipitation is more obvious change, global warming has led to a large number of glaciers over the Tibetan Plateau, and then changed to the area of water cycle and soil moisture. The Tibetan plateau with different gradient of warming water experiment platform based on using quantitative PCR, (qPCR), the terminal restriction fragment length polymorphism (T-RFLP) method, the temperature increase of 1,3,13 months (1,2,4 C) and precipitation (100% increase of precipitation, precipitation reduced 50%) nirK on the Tibetan Plateau alpine meadow soil NIR, S, nosZ denitrifying bacteria community structure and abundance, influence diversity. The results show that nirS and nosZ gene copy number in the area is about 10 times of nirK; warming, precipitation and temperature increase and precipitation increased by treatment of nirK, NIR, S, nosZ and denitrifying bacteria abundance were not There is obvious effect; influence of sampling time on the abundance of nosZ gene significantly, but the nirK and NIR S gene abundance did not significantly affect the denitrifying bacteria community structure of.NirS and nosZ on the growth response of mild precipitation changes than nirK denitrifying bacteria sensitive nirS and nosZ denitrifying bacteria in warming or changes in water treatment for 1 months when the community structure changed significantly, but the denitrifying bacteria community structure of nirK in the treatment of 1 months, the community structure did not change significantly with the treatment time increased, community structure gradually changed; between warming and precipitation changes on nirK, nirS, effect of denitrifying bacteria community structure nosZ regulation a temperature increase of 2 degrees C and compared; the precipitation increased by 100%, the different sampling time of nirK, nirS, effect of denitrifying bacteria community structure of nosZ is dominant, and by the increasing effects of mild changes in precipitation and the temperature increasing; 2 treatment compared to increase The precipitation of 100% pairs of nirK and nirS community structure has more influence on the effect of sampling time, the precipitation increased by 100% and 2 DEG C temperature increase significantly influence the sampling time effect of nosZ community structure. Increasing temperature and precipitation changes on nirK, nirS, nosZ denitrifying bacteria has no obvious effect on species diversity, different sampling time on diversity there is no significant effect.

【學(xué)位授予單位】：中國(guó)礦業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S812.2

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本文編號(hào)：1708101