本文選題：豬　切入點(diǎn)：ChREBP　出處：《山西農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：ChREBP作為一種轉(zhuǎn)錄因子,主要結(jié)合在糖酵解和脂肪合成相關(guān)基因的啟動(dòng)子上,調(diào)控糖脂代謝相關(guān)酶的轉(zhuǎn)錄。本研究利用qRT-PCR和Western blotting技術(shù),選取馬身豬和大白豬初生至180日齡(1,30,60,90,120,150,180)7個(gè)生長(zhǎng)點(diǎn),檢測(cè)ChREBP mRNA和蛋白在肝臟、背最長(zhǎng)肌和背部皮下脂肪等3種組織中的發(fā)育性表達(dá)規(guī)律。結(jié)果如下：1. ChREBP mRNA在肝臟和背最長(zhǎng)肌中的表達(dá)模式均為波浪形。馬身豬和大白豬肝臟中表達(dá)最高峰分別為30和60日齡；兩豬種背最長(zhǎng)肌中1日齡的表達(dá)量極顯著低于其他日齡,分別在60和90日齡表達(dá)最高;皮下脂肪組織中,兩豬種均高表達(dá)于生長(zhǎng)后期。品種間比較,該基因的表達(dá)量在馬身豬肝臟30、90、150、180日齡和背最長(zhǎng)肌整個(gè)階段均極顯著高于大白豬,肝臟其余階段和皮下脂肪整個(gè)階段,大白豬顯著高于馬身豬。組織間比較,馬身豬整體表現(xiàn)為：肝臟背最長(zhǎng)肌脂肪；大白豬中背最長(zhǎng)肌中表達(dá)量最少,優(yōu)勢(shì)表達(dá)組織分兩個(gè)階段,生長(zhǎng)前期為肝臟,后期為背部皮下脂肪。2. ChREBP蛋白在馬身豬肝臟90日齡、大白豬60日齡表達(dá)最高；兩豬種背最長(zhǎng)肌中1日齡的表達(dá)量最低,隨后急劇增長(zhǎng);皮下脂肪組織中30日齡的表達(dá)量最高。品種間比較,該蛋白表達(dá)量在馬身豬肝臟和皮下脂肪中各階段均低于大白豬;背最長(zhǎng)肌中馬身豬的表達(dá)量高于大白豬,90日齡表達(dá)量除外。組織間比較,兩豬種背最長(zhǎng)肌中的表達(dá)量極顯著高于另兩個(gè)組織,大白豬1日齡和60日齡階段除外。本文結(jié)果表明：ChREBP mRNA和蛋白在不同品種、不同組織和不同日齡中表達(dá)具有顯著差異,提示該基因?qū)ωi體內(nèi)脂肪沉積的調(diào)控起重要作用。
[Abstract]:As a transcription factor, ChREBP mainly binds to the promoter of glycolytic and fat biosynthesis related genes to regulate the transcription of glycolipid metabolism-related enzymes.In this study, qRT-PCR and Western blotting techniques were used to detect the developmental expression of ChREBP mRNA and protein in liver, longissimus dorsi muscle and subcutaneous fat of three kinds of tissues.The result is as follows: 1.The expression pattern of ChREBP mRNA in liver and longissimus dorsi muscle was wavy.The highest expression peaks were 30 and 60 days old in liver of Mashen pig and large white pig, respectively, the expression of 1 day old in the longissimus dorsi muscle of both breeds was significantly lower than that of other days old, and the highest expression was in 60 and 90 days old, respectively; in subcutaneous adipose tissue, the highest expression was found in the subcutaneous adipose tissue.Both breeds were highly expressed in the late stage of growth.The expression level of the gene was significantly higher in the 30 ~ 90150 ~ 180 days old of liver and the whole stage of the longissimus dorsi muscle than in the whole stage of liver and subcutaneous fat, and the expression of the gene was significantly higher in the whole stage of liver and subcutaneous fat than that of the horse body pig.Tissue comparison showed that the overall performance of Ma-Shen pig was: liver longissimus dorsi muscle fat; in large white pig, the expression of longissimus dorsi muscle was the least, and the dominant expression tissue was divided into two stages: liver in pregrowth stage and subcutaneous fat in back in later stage.The expression of ChREBP protein was the highest in the liver of Ma-Shen pig at 90 days old and the highest in the white pig at 60 days old, the lowest in the longissimus dorsi muscle of the two breeds at 1 day old, and the highest in the subcutaneous adipose tissue at the age of 30 days.The expression of this protein was lower in liver and subcutaneous fat than that in white pig, except that in longissimus dorsi muscle was higher than that in white pig at 90 days old.The expression level of the longissimus dorsi muscle was significantly higher in the two pigs than in the other two tissues, except for the 1-day and 60-day-old stage.The results showed that there were significant differences in the expression of ChREBP mRNA and protein in different breeds, tissues and days of age, suggesting that the gene plays an important role in the regulation of fat deposition in pigs.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 盧建雄;楊具田;劉翊中;臧榮鑫;潘和平;白春明;王克賢;;不同飼養(yǎng)環(huán)境合作豬脂肪組織生脂基因mRNA表達(dá)水平的比較研究[J];中國(guó)畜牧獸醫(yī);2006年06期

2 黃森;林文_";翁錫全;劉建紅;周志宏;;高住低練對(duì)大鼠骨骼肌丙酮酸激酶及其轉(zhuǎn)錄調(diào)節(jié)因子的影響[J];成都體育學(xué)院學(xué)報(bào);2013年07期

3 黃森;林文_";翁錫全;劉建紅;周志宏;;有氧訓(xùn)練對(duì)大鼠腓腸肌ChREBP及其調(diào)節(jié)因子的影響[J];山東體育學(xué)院學(xué)報(bào);2013年05期

4 王艷莉;翟成凱;陳晗;王曉飛;;高脂飼料喂養(yǎng)大鼠誘導(dǎo)的脂代謝紊亂對(duì)糖代謝及炎癥因子的影響[J];衛(wèi)生研究;2008年02期

5 劉建紅;黃森;林文_";翁錫全;;有氧運(yùn)動(dòng)對(duì)高糖高脂膳食大鼠肝臟碳水化合物反應(yīng)元件結(jié)合蛋白mRNA表達(dá)的影響[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2008年05期

6 劉長(zhǎng)金;劉磊;柯大智;林雪梅;姜蓉;胥文春;左國(guó)偉;王建偉;;高果糖引起的脂肪肝大鼠腎臟脂質(zhì)合成相關(guān)基因和蛋白的表達(dá)[J];中國(guó)細(xì)胞生物學(xué)學(xué)報(bào);2014年04期

相關(guān)博士學(xué)位論文 前1條

1 張金偉;能量來源對(duì)產(chǎn)蛋雞肝臟脂肪代謝的影響及其機(jī)制研究[D];四川農(nóng)業(yè)大學(xué);2009年

相關(guān)碩士學(xué)位論文 前2條

1 唐慧;鵝ChREBP基因克隆及其在肝脂肪變性中的作用初步研究[D];四川農(nóng)業(yè)大學(xué);2010年

2 陳灰;脂聯(lián)素激活A(yù)MPK信號(hào)通路調(diào)控奶牛肝細(xì)胞脂代謝的相關(guān)機(jī)制[D];吉林大學(xué);2013年

，

本文編號(hào)：1706991