本文選題：公鵝　切入點(diǎn)：不同遺傳基礎(chǔ)　出處：《吉林農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本試驗(yàn)結(jié)合養(yǎng)鵝生產(chǎn)中存在的技術(shù)難題,設(shè)計(jì)了5個(gè)不同遺傳基礎(chǔ)的公鵝試驗(yàn)組。通過(guò)飼養(yǎng)孵化試驗(yàn),檢測(cè)了試驗(yàn)組間的受精率和使用時(shí)間上的差異;通過(guò)HE染色組織切片法,比較了繁殖期內(nèi)不同試驗(yàn)組公鵝睪丸組織形態(tài)學(xué)變化的差異;通過(guò)基因差異表達(dá)分析,比較了不同試驗(yàn)組GnRH和PRL的mRNA差異表達(dá)。從而為公鵝的繁殖性能優(yōu)化選擇提供了理論參考依據(jù)。研究結(jié)果如下:(1)組間公鵝受精率:繁殖前期差異顯著(p㩳0.05),以Ⅴ組公鵝最高,繁殖中期差異不顯著(p㧐0.05),平均值以Ⅴ組公鵝最大。繁殖末期差異顯著(p㩳0.05),以Ⅴ組公鵝受精率下降最慢;有效配種天數(shù):Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ組公鵝分別為99、81、99、81、118d,Ⅴ組公鵝較Ⅰ、Ⅱ、Ⅲ、Ⅳ組公鵝有效配種天數(shù)分別延長(zhǎng)19、37、19、37d。(2)繁殖期內(nèi)公鵝睪丸組織形態(tài)學(xué)變化:繁殖初期5個(gè)試驗(yàn)組公鵝的睪丸重、曲細(xì)精管直徑、生殖上皮高度、精子密度等指標(biāo)均呈現(xiàn)上升現(xiàn)象;繁殖中期,組間公鵝睪丸的各指標(biāo)增加值雖不相同,但均在此時(shí)期達(dá)到了最大值;繁殖末期,Ⅰ、Ⅱ、Ⅲ、Ⅳ組公鵝睪丸指標(biāo)值下降較快,Ⅴ組公鵝睪丸指標(biāo)值下降相對(duì)較慢,與此時(shí)期受精率一致。(3)GnRH和PRL表達(dá)變化:整個(gè)繁殖期內(nèi),兩基因在5個(gè)試驗(yàn)組公鵝睪丸內(nèi)均有表達(dá),且規(guī)律相同。從繁殖初期、中期至末期,GnRH表達(dá)量呈現(xiàn)出先增多后減少,繁殖中期表達(dá)最高;PRL表達(dá)量為先減少后增多,繁殖中期表達(dá)最低。GnRH對(duì)公鵝繁殖性能有促進(jìn)作用,PRL則起抑制作用。
[Abstract]:Combining with the technical problems existing in the production of geese, five test groups of male geese with different genetic basis were designed.The differences of fertilization rate and using time among the test groups were detected by feeding and hatching experiment, and the morphological changes of testis in different test groups were compared by HE staining tissue section method.MRNA differential expression of GnRH and PRL in different groups was compared by gene differential expression analysis.Thus it provides a theoretical reference for the selection of reproductive performance of male geese.The results were as follows: fertilization rate of male geese in the early stage of reproduction was significantly different from that in group V, the highest in group 鈪，

本文編號(hào)：1698549